Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,480 G→C 20.9% G709G (GGC→GGG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4800G→C20.9% 108.7 ‑2.9 ‑0.2197G709G (GGC→GGG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (14/27):  ref base (80/75):  total (95/102)
Fisher's exact test strand distribution p-value = 5.40e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.24e-01

Only 100 of 217 total aligned reads displayed.

CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCA  >  NC_014034/681382‑681580
                                                                                                  |                                                                                                      
   gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                                  <  1:690547/101‑1 (MQ=255)
    ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                                                 >  1:3072881/1‑101 (MQ=255)
    ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                                                 <  2:4616803/101‑1 (MQ=255)
     cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                >  1:3875933/1‑101 (MQ=255)
      aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                               >  1:3997497/1‑101 (MQ=255)
       tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgc                                                                                              <  2:2505273/101‑1 (MQ=255)
          tggcttagcgcagcccGGAGAGCGCCGTCGCGCGGATCTACGTCAGATCTTCCGAATAGAGCGCCGCGTTCTGCGTGGTGGTCAGCGCGCCG.ATCTGCGcc                                                                                           <  2:2027141/95‑1 (MQ=19)
           ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGGGCCG.ATCTGCGCCg                                                                                          >  2:2361997/1‑101 (MQ=255)
           ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                                          >  1:4486761/1‑101 (MQ=255)
            gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGa                                                                                         >  1:3513976/1‑101 (MQ=255)
              cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                       >  2:866258/1‑101 (MQ=255)
               gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                      <  1:547224/101‑1 (MQ=255)
               gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                      <  2:2828017/101‑1 (MQ=255)
                  cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                   <  1:871152/101‑1 (MQ=255)
                   ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGa                                                                                  >  1:1621578/1‑101 (MQ=255)
                      gcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgc                                                                               <  1:3949258/101‑1 (MQ=255)
                       cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgc.g                                                                             >  1:4137795/1‑101 (MQ=255)
                        ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACgc.gc                                                                            >  2:1169326/1‑101 (MQ=255)
                         cGGAGAGCTCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                           <  1:4246863/101‑1 (MQ=255)
                         cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                           <  1:4912142/101‑1 (MQ=255)
                          ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                                          >  1:127957/1‑101 (MQ=255)
                            agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                        <  1:1019758/101‑1 (MQ=255)
                            agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                        >  2:3818583/1‑101 (MQ=255)
                             gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCt                                                                       <  2:341714/101‑1 (MQ=255)
                              agCGCCGTCGCGCTGATCTGCGCTAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTg                                                                      <  2:1829619/101‑1 (MQ=255)
                                 gcCGTCGCGCTGATCTGCGCCAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTgcgc                                                                   >  1:4490819/1‑101 (MQ=255)
                                   cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                                                 <  2:1850689/101‑1 (MQ=255)
                                   cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                                                 >  2:4075235/1‑101 (MQ=255)
                                    gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                                                >  1:3677838/1‑101 (MQ=255)
                                         gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCa                                                           <  1:3279146/101‑1 (MQ=255)
                                          cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAg                                                          >  2:3578378/1‑101 (MQ=255)
                                          cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGACTGCGCCGTCGCCAg                                                          >  1:4445062/1‑101 (MQ=255)
                                           tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GGCGCCTGCGCCGTCGTCAgc                                                         >  1:2964804/1‑101 (MQ=255)
                                           ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                                         <  1:1093843/92‑1 (MQ=2)
                                            gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAgcg                                                        >  2:4562526/1‑101 (MQ=255)
                                               ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCa                                                     <  1:5008976/96‑1 (MQ=2)
                                                 gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                   <  2:4261747/101‑1 (MQ=255)
                                                 gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCTTGCTCGACAGGGCCAGA.AGCTGCGTCGAGCCCAG.CGCGGCCGCCTGCGCCGTCGTCAGCCCCACg                                                   >  2:1636690/4‑101 (MQ=2)
                                                  cgcCAGATCCGCCGCCTCGAGGGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                                  >  1:3717894/1‑101 (MQ=255)
                                                  cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                                  >  1:794114/1‑101 (MQ=255)
                                                   gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                 <  1:18655/101‑1 (MQ=255)
                                                    ccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                <  2:2788658/101‑1 (MQ=9)
                                                    ccAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                >  1:2738498/1‑101 (MQ=255)
                                                       gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                             >  2:4499916/1‑101 (MQ=255)
                                                        aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                            >  1:3870981/1‑101 (MQ=255)
                                                           cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                         >  2:599939/1‑101 (MQ=255)
                                                            gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCggg                                        >  2:3741002/1‑100 (MQ=255)
                                                              cgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGNCACGATGTCATCGGTGc                                      >  2:1535291/1‑101 (MQ=255)
                                                               gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                     <  2:2089590/95‑1 (MQ=12)
                                                               gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                     >  1:1498377/7‑101 (MQ=9)
                                                               gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                     <  2:4154348/101‑1 (MQ=255)
                                                               gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                     >  2:173597/1‑101 (MQ=255)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCCCGATGTCATCGGTGccga                                   >  2:4967358/5‑99 (MQ=9)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                   >  2:932608/5‑99 (MQ=9)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                   >  2:183774/5‑99 (MQ=2)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                   <  2:1078842/97‑3 (MQ=9)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGAGCCAGA.AGATGCGTCGATCCCAGCGAC.GCCGCCTGCGCCGTCTTCAGAGCCACGTTGTCATCGGTGcctt                                   >  2:2921704/5‑99 (MQ=255)
                                                                  tccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgag                                  <  1:3852182/98‑4 (MQ=9)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                 >  2:5019028/1‑101 (MQ=255)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                 >  2:4819878/1‑101 (MQ=255)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                 <  2:4749914/101‑1 (MQ=255)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                 <  1:330870/101‑1 (MQ=255)
                                                                    gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                <  1:2026676/101‑1 (MQ=255)
                                                                    gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                <  1:2579101/101‑1 (MQ=255)
                                                                    caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                <  2:1892595/100‑6 (MQ=9)
                                                                     aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc                               >  1:1315237/1‑101 (MQ=255)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                               <  2:3834733/101‑1 (MQ=9)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                               >  1:2113145/1‑101 (MQ=2)
                                                                       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                             >  1:4160371/1‑99 (MQ=2)
                                                                       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                             >  2:2498584/1‑99 (MQ=2)
                                                                         ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                           <  2:3644085/101‑5 (MQ=2)
                                                                         ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGGCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                           <  2:394344/101‑5 (MQ=9)
                                                                          cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                          <  1:229757/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                         <  2:1467376/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                         <  1:4710408/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                         <  1:3971375/101‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCGGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                       <  2:794012/101‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                       <  2:514597/101‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                       <  2:3072643/101‑1 (MQ=255)
                                                                             gATCGGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                       <  2:3513706/101‑1 (MQ=255)
                                                                              accTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgc                      >  2:3993603/3‑101 (MQ=2)
                                                                               tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcccc                     <  1:2885131/101‑1 (MQ=255)
                                                                                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                    >  2:762985/1‑101 (MQ=255)
                                                                                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                    >  2:1405893/1‑101 (MQ=255)
                                                                                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                    <  2:4872820/101‑1 (MQ=255)
                                                                                 tGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                   <  2:3677560/101‑1 (MQ=255)
                                                                                  gCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                  >  2:3277184/1‑101 (MQ=255)
                                                                                  gCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                  >  2:3974770/1‑101 (MQ=255)
                                                                                      gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc              >  1:1947071/1‑101 (MQ=255)
                                                                                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc             <  1:1841773/101‑1 (MQ=255)
                                                                                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAGCGCCGCCGTGcc             >  1:1380503/1‑101 (MQ=255)
                                                                                        tGGTCCGCGCGCCG.ATCTGCGCCGGGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc            <  2:2417761/101‑1 (MQ=255)
                                                                                         tgTCAGCGCGCCG.ATCTGCGCCGACCCCAGACGC.GCCGCCTGGGCCGTCGGCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCa           <  2:3304852/100‑1 (MQ=37)
                                                                                          gTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg          <  1:4795589/101‑1 (MQ=255)
                                                                                           tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc         <  1:1918060/101‑1 (MQ=255)
                                                                                           tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc         >  1:2828010/1‑101 (MQ=255)
                                                                                            cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCaacc        >  2:5039061/1‑98 (MQ=255)
                                                                                             cgcgcgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc       <  1:2362161/100‑1 (MQ=255)
                                                                                                 cgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc   <  1:460680/101‑1 (MQ=255)
                                                                                                 cgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc   <  1:2540003/101‑1 (MQ=255)
                                                                                                  |                                                                                                      
CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCA  >  NC_014034/681382‑681580

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG