Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,482 C→G 20.2% G709R (GGC→CGA)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4820C→G20.2% 106.4 ‑2.5 ‑0.7204G709R (GGC→CGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (14/27):  ref base (81/81):  total (96/108)
Fisher's exact test strand distribution p-value = 8.08e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.98e-01

Only 100 of 219 total aligned reads displayed.

CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAC  >  NC_014034/681382‑681581
                                                                                                    |                                                                                                     
   gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                                   <  1:690547/101‑1 (MQ=255)
   gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                                   <  2:4790044/101‑1 (MQ=255)
    ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                                                  >  1:3072881/1‑101 (MQ=255)
     cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                 >  1:3875933/1‑101 (MQ=255)
      aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                                >  1:3997497/1‑101 (MQ=255)
      aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                                >  2:5014923/1‑101 (MQ=255)
        cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcg                                                                                              >  2:3969248/1‑101 (MQ=255)
        cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGACCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcg                                                                                              <  2:2595383/101‑1 (MQ=255)
          tggcttagcgcagcccGGAGAGCGCCGTCGCGCGGATCTACGTCAGATCTTCCGAATAGAGCGCCGCGTTCTGCGTGGTGGTCAGCGCGCCG.ATCTGCGcc                                                                                            <  2:2027141/95‑1 (MQ=19)
           ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGGGCCG.ATCTGCGCCg                                                                                           >  2:2361997/1‑101 (MQ=255)
           ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                                           >  1:4486761/1‑101 (MQ=255)
            gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGa                                                                                          >  1:3513976/1‑101 (MQ=255)
             ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAg                                                                                         <  2:3735592/101‑1 (MQ=255)
              cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                        <  2:53838/101‑1 (MQ=255)
              cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                        >  2:866258/1‑101 (MQ=255)
               gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                       <  2:2828017/101‑1 (MQ=255)
               gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                       <  1:547224/101‑1 (MQ=255)
                 gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCa                                                                                     <  2:3982639/101‑1 (MQ=255)
                  cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                    >  1:3356525/1‑101 (MQ=255)
                  cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                    <  1:871152/101‑1 (MQ=255)
                   ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGa                                                                                   >  1:1621578/1‑101 (MQ=255)
                       cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgc.g                                                                              >  1:4137795/1‑101 (MQ=255)
                        ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACgc.gc                                                                             >  2:1169326/1‑101 (MQ=255)
                         cGGAGAGCTCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                            <  1:4246863/101‑1 (MQ=255)
                          ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                                           >  1:127957/1‑101 (MQ=255)
                          ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                                           >  2:330817/1‑101 (MQ=255)
                           gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccgc                                                                          >  1:4873147/1‑101 (MQ=255)
                            agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                         <  1:1019758/101‑1 (MQ=255)
                            agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                         >  2:3818583/1‑101 (MQ=255)
                            agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                         >  2:4523938/1‑101 (MQ=255)
                             gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCt                                                                        <  2:341714/101‑1 (MQ=255)
                              agCGCCGTCGCGCTGATCTGCGCTAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTg                                                                       <  2:1829619/101‑1 (MQ=255)
                                 gcCGTCGCGCTGATCTGCGCCAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTgcgc                                                                    >  1:4490819/1‑101 (MQ=255)
                                   cgccgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATGTGCGTGCTGGTCAGGGCGCCG.ATCTGCGCCGAGCCAAGACGC.GCCGCCTGCGCcg                                                                  <  2:619325/98‑1 (MQ=25)
                                   cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                                                  <  2:1850689/101‑1 (MQ=255)
                                    gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                                                 >  2:48974/1‑101 (MQ=255)
                                    gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCATACGCcgt                                                                 >  2:4007452/1‑101 (MQ=255)
                                          cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAg                                                           >  2:3578378/1‑101 (MQ=255)
                                           tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GGCGCCTGCGCCGTCGTCAgc                                                          >  1:2964804/1‑101 (MQ=255)
                                           ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                                          <  1:1093843/92‑1 (MQ=2)
                                               ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCa                                                      <  1:5008976/96‑1 (MQ=2)
                                                cgcgcCAGATCCCCCGCCCCGAGCGCCGCCATCTTCCTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                                     <  2:5045812/100‑1 (MQ=25)
                                                cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                                     <  2:4606429/97‑1 (MQ=2)
                                                 gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                    >  2:3791042/1‑101 (MQ=255)
                                                 gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                    <  2:4261747/101‑1 (MQ=255)
                                                 gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCTTGCTCGACAGGGCCAGA.AGCTGCGTCGAGCCCAG.CGCGGCCGCCTGCGCCGTCGTCAGCCCCACg                                                    >  2:1636690/4‑101 (MQ=2)
                                                 gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTGGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                    <  2:3281452/98‑1 (MQ=2)
                                                  cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                                   >  1:794114/1‑101 (MQ=255)
                                                  caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGACGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGGGCCACGa                                                   >  2:5021035/3‑101 (MQ=9)
                                                   gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                  <  1:18655/101‑1 (MQ=255)
                                                   accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                  <  1:3689095/100‑1 (MQ=2)
                                                    ccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                 <  2:2788658/101‑1 (MQ=9)
                                                    ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                 >  1:3937935/1‑101 (MQ=255)
                                                        aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                             >  1:3870981/1‑101 (MQ=255)
                                                          ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                           >  1:4419831/1‑101 (MQ=255)
                                                          ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                           <  1:3280713/101‑1 (MQ=255)
                                                              cgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGNCACGATGTCATCGGTGc                                       >  2:1535291/1‑101 (MQ=255)
                                                               gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                      >  1:1498377/7‑101 (MQ=9)
                                                               gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                      <  2:2089590/95‑1 (MQ=12)
                                                               gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                      >  2:173597/1‑101 (MQ=255)
                                                               gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                      <  2:4154348/101‑1 (MQ=255)
                                                                ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATCTCATCGGTGCCa                                     <  1:2742357/101‑1 (MQ=255)
                                                                ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATCTCATCGGTGCCa                                     <  1:2732474/101‑1 (MQ=255)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                    >  2:932608/5‑99 (MQ=9)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                    >  2:183774/5‑99 (MQ=2)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                    <  2:1078842/97‑3 (MQ=9)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGAGCCAGA.AGATGCGTCGATCCCAGCGAC.GCCGCCTGCGCCGTCTTCAGAGCCACGTTGTCATCGGTGcctt                                    >  2:2921704/5‑99 (MQ=255)
                                                                  tccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgag                                   <  1:3852182/98‑4 (MQ=9)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                  >  2:4819878/1‑101 (MQ=255)
                                                                     aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc                                >  1:1315237/1‑101 (MQ=255)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                <  2:4721859/101‑1 (MQ=9)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                <  2:3834733/101‑1 (MQ=9)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                >  1:2113145/1‑101 (MQ=2)
                                                                       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                              >  1:4160371/1‑99 (MQ=2)
                                                                       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                              >  2:2498584/1‑99 (MQ=2)
                                                                          cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                           <  1:229757/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                          <  1:4710408/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                          <  2:1467376/101‑1 (MQ=255)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                          <  1:3969540/101‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCGGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                        <  2:794012/101‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                        <  2:3072643/101‑1 (MQ=255)
                                                                             gATCGGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                        <  2:3513706/101‑1 (MQ=255)
                                                                               tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcccc                      <  1:2885131/101‑1 (MQ=255)
                                                                                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                     >  2:1405893/1‑101 (MQ=255)
                                                                                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                     >  2:762985/1‑101 (MQ=255)
                                                                                 tGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                    <  2:1955148/101‑1 (MQ=255)
                                                                                      gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc               >  1:2064511/1‑101 (MQ=255)
                                                                                      gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc               >  1:1947071/1‑101 (MQ=255)
                                                                                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc              <  1:1841773/101‑1 (MQ=255)
                                                                                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAGCGCCGCCGTGcc              >  1:1380503/1‑101 (MQ=255)
                                                                                        tGGTCCGCGCGCCG.ATCTGCGCCGGGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc             <  2:2417761/101‑1 (MQ=255)
                                                                                         ggTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa            <  2:4137478/101‑1 (MQ=255)
                                                                                          gTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg           <  1:4795589/101‑1 (MQ=255)
                                                                                           tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc          >  1:2828010/1‑101 (MQ=255)
                                                                                           tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc          <  1:1918060/101‑1 (MQ=255)
                                                                                            cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg         >  2:3677434/1‑101 (MQ=255)
                                                                                             cgcgcgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc        <  1:2362161/100‑1 (MQ=255)
                                                                                                 cgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc    <  1:460680/101‑1 (MQ=255)
                                                                                                 cgcCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc    <  1:2540003/101‑1 (MQ=255)
                                                                                                   ccG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc  >  1:365400/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAC  >  NC_014034/681382‑681581

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG