Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,483 G→A 20.1% I708I (ATC→CGA)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4830G→A20.1% 114.1 ‑2.5 ‑0.1204I708I (ATC→ATT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (14/27):  ref base (82/81):  total (96/108)
Fisher's exact test strand distribution p-value = 8.00e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.79e-01

Only 100 of 221 total aligned reads displayed.

CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583
                                                                                                    |                                                                                                     
  gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                                                     <  2:4790044/101‑1 (MQ=255)
     aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGCGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                                                  >  1:4751252/1‑101 (MQ=255)
     aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                                                  >  2:5014923/1‑101 (MQ=255)
     aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                                                  >  1:3997497/1‑101 (MQ=255)
      tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                                                                 <  2:2505273/101‑1 (MQ=255)
      tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                                                                 >  2:2026532/1‑101 (MQ=255)
        gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcgc                                                                                               <  1:2085021/101‑1 (MQ=255)
          ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCg                                                                                             >  1:4486761/1‑101 (MQ=255)
             cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                                                          >  2:866258/1‑101 (MQ=255)
             cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                                                          <  2:53838/101‑1 (MQ=255)
             cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                                                          >  1:573455/1‑101 (MQ=255)
             cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                                                          <  1:3872949/101‑1 (MQ=255)
                 cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAg                                                                                      <  1:519500/101‑1 (MQ=255)
                  ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGa                                                                                     >  1:1621578/1‑101 (MQ=255)
                     gcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGAcgc                                                                                  <  1:3949258/101‑1 (MQ=255)
                         ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGc.gccg                                                                             >  1:127957/1‑101 (MQ=255)
                           agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.gccgcc                                                                           >  2:3818583/1‑101 (MQ=255)
                           agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.gccgcc                                                                           <  1:1019758/101‑1 (MQ=255)
                             agCGCCGTCGCGCTGATCTGCGCTAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTg                                                                         <  2:1829619/101‑1 (MQ=255)
                                   gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                                                   >  1:3677838/1‑101 (MQ=255)
                                   gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCATACGCcgt                                                                   >  2:4007452/1‑101 (MQ=255)
                                    tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.TCCGCCTGCGCcgtc                                                                  <  1:4995485/101‑1 (MQ=255)
                                       cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCgtcgtc                                                               >  1:4803815/1‑101 (MQ=255)
                                         cggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAg                                                             <  1:3216274/91‑1 (MQ=2)
                                         cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAg                                                             >  2:3578378/1‑101 (MQ=255)
                                         cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGACTGCGCCGTCGCCAg                                                             >  1:4445062/1‑101 (MQ=255)
                                          tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAgc                                                            >  1:4154665/1‑101 (MQ=255)
                                          ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                                            <  2:2056722/92‑1 (MQ=2)
                                          ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                                            <  1:1093843/92‑1 (MQ=2)
                                               cgcgcCAGATCCCCCGCCCCGAGCGCCGCCATCTTCCTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  2:5045812/100‑1 (MQ=25)
                                               cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  2:4606429/97‑1 (MQ=2)
                                               cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  1:901341/97‑1 (MQ=2)
                                                gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                      <  2:4261747/101‑1 (MQ=255)
                                                gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCTTGCTCGACAGGGCCAGAAGCTGCGTCGAGCCCAG.CGCGGCCGCCTGCGCCGTCGTCAGCCCCACg                                                      >  2:1636690/4‑101 (MQ=2)
                                                gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                      <  1:3925425/98‑1 (MQ=2)
                                                gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                                      <  2:2598648/98‑1 (MQ=2)
                                                 cgcCAGATCCGCCGCCTCGAGGGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                                     >  1:3717894/1‑101 (MQ=255)
                                                 caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGACGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGGGCCACGa                                                     >  2:5021035/3‑101 (MQ=9)
                                                  gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                    <  1:18655/101‑1 (MQ=255)
                                                   ccAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                   >  1:2738498/1‑101 (MQ=255)
                                                    cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                  <  1:3513890/101‑1 (MQ=255)
                                                      gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                >  2:4499916/1‑101 (MQ=255)
                                                        tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                              <  1:4607355/101‑1 (MQ=255)
                                                        tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGATCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                              >  2:2825426/1‑101 (MQ=255)
                                                         ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                             <  1:4181143/101‑1 (MQ=255)
                                                         ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                             <  1:3280713/101‑1 (MQ=255)
                                                         ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                             <  1:3046268/101‑1 (MQ=255)
                                                          cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                            >  2:599939/1‑101 (MQ=255)
                                                           gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCggg                                           >  2:3741002/1‑100 (MQ=255)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         <  1:4573722/94‑1 (MQ=9)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         <  1:2131301/94‑1 (MQ=9)
                                                              gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        >  2:173597/1‑101 (MQ=255)
                                                              gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  2:4154348/101‑1 (MQ=255)
                                                               ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                       <  1:2335056/101‑1 (MQ=255)
                                                                ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                      >  2:183774/5‑99 (MQ=2)
                                                                ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                      >  2:932608/5‑99 (MQ=9)
                                                                ttccaGCGCCGCAACCTGCGTGCTCGACAGAGCCAGAAGATGCGTCGATCCCAGCGAC.GCCGCCTGCGCCGTCTTCAGAGCCACGTTGTCATCGGTGcctt                                      >  2:2921704/5‑99 (MQ=255)
                                                                 tccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgag                                     <  1:3852182/98‑4 (MQ=9)
                                                                  cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                    >  2:4819878/1‑101 (MQ=255)
                                                                  cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                    >  1:852211/1‑101 (MQ=255)
                                                                   gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                   <  1:2579101/101‑1 (MQ=255)
                                                                   caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                   <  2:1892595/100‑6 (MQ=9)
                                                                    aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc                                  >  1:1315237/1‑101 (MQ=255)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  <  1:201605/101‑1 (MQ=9)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  <  2:4721859/101‑1 (MQ=9)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  <  2:3834733/101‑1 (MQ=9)
                                                                      cgccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                >  2:2498584/1‑99 (MQ=2)
                                                                        ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                              <  2:3644085/101‑5 (MQ=2)
                                                                        ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGGCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                              <  2:394344/101‑5 (MQ=9)
                                                                          gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                            <  2:1467376/101‑1 (MQ=255)
                                                                          gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                            <  1:3971375/101‑1 (MQ=255)
                                                                           cgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAta                           >  1:3619027/1‑100 (MQ=255)
                                                                           cgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                           >  2:1597303/1‑101 (MQ=255)
                                                                            gATCTGCGTGCTGGTCGGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          <  2:794012/101‑1 (MQ=255)
                                                                            gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          <  2:3072643/101‑1 (MQ=255)
                                                                            gATCGGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          <  2:3513706/101‑1 (MQ=255)
                                                                              tCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                        <  2:1093129/101‑1 (MQ=255)
                                                                               cTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                       <  2:4872820/101‑1 (MQ=255)
                                                                               cTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                       >  2:1405893/1‑101 (MQ=255)
                                                                               cTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                       >  2:762985/1‑101 (MQ=255)
                                                                                tGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                      <  2:3677560/101‑1 (MQ=255)
                                                                                 gCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                     >  2:3277184/1‑101 (MQ=255)
                                                                                 gCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                     >  2:3974770/1‑101 (MQ=255)
                                                                                   gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCCTCGGTGCAAAAGCCGCGGATCGCCGCCGt                   >  2:2361677/1‑101 (MQ=255)
                                                                                      cTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                <  1:1841773/101‑1 (MQ=255)
                                                                                      cTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAGCGCCGCCGTGcc                >  1:1380503/1‑101 (MQ=255)
                                                                                       tGGTCCGCGCGCCGATCTGCGCCGGGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc               <  2:2417761/101‑1 (MQ=255)
                                                                                       tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc               >  1:1529280/1‑101 (MQ=255)
                                                                                       tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc               <  1:4384760/101‑1 (MQ=255)
                                                                                        tgTCAGCGCGCCGATCTGCGCCGACCCCAGACGC.GCCGCCTGGGCCGTCGGCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCa              <  2:3304852/100‑1 (MQ=37)
                                                                                        ggTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa              <  2:4137478/101‑1 (MQ=255)
                                                                                          tCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc            <  1:1918060/101‑1 (MQ=255)
                                                                                            aGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc          >  1:2811074/1‑101 (MQ=255)
                                                                                             gcgcgcCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgcc         <  1:785559/101‑1 (MQ=255)
                                                                                               gcgcCGATCTGCGCCGAGCCCGGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc       >  1:2862564/1‑101 (MQ=255)
                                                                                                cgcCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc      <  1:2540003/101‑1 (MQ=255)
                                                                                                 gcCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa     >  2:3324296/1‑101 (MQ=255)
                                                                                                    gATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg  >  1:2059540/1‑101 (MQ=255)
                                                                                                    gATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg  >  2:1259790/1‑101 (MQ=255)
                                                                                                    gATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg  >  1:2919565/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG