Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,625	A→G	100%	intergenic (‑79/+41)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,625	0	A→G	100.0%	22.4	7	intergenic (‑79/+41)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (0/0):  total (7/0)

CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAT  >  NC_014034/774585‑774702                                               |                                                                                  cGGGGGGGCGCCGGGCCGCAGGCCCGGCGCCCCCCCGCCCGGGGCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCcg                           >  1:4468860/1‑101 (MQ=255)                   caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt         >  1:88244/9‑101 (MQ=255)                   caggcccggcgcCCCCCCGCCCGCGTCGGACCGGCCCTGCC.GGCC.CGACCCCACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACggt         >  2:2452122/9‑99 (MQ=255)                    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGG.C.CGACCCTACCGGCTCTTATCCTGCGATCACTTCGCCCGCGACCCGACGGCCACGTTc        >  1:4561874/8‑101 (MQ=255)                     ggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTcc       >  1:1432018/7‑101 (MQ=255)                       cccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACGTTCCAg     >  2:4668931/5‑101 (MQ=255)                          ggcgcCCCCCCGCCCGGGACGGACCGGCCCTGCC.CG.TCCGACCCTACGGTCTCTTATCCTGCGATCCCCTCGCCCGCGACCCGTCGGCCCCGTTCCAATAt  >  2:2109406/2‑101 (MQ=255)                                               |                                                                                  CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAT  >  NC_014034/774585‑774702 Base quality scores:  ATCG  < 3 ≤  ATCG  < 6 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 38 ≤  ATCG