Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 915,071 T→C 4.8% intergenic (+52/+37) recN → / ← RCAP_rcc00830 DNA repair protein RecN/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034915,0710T→C 4.8% 13.9 ‑1.8 ‑0.4187intergenic (+52/+37)recN/RCAP_rcc00830DNA repair protein RecN/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/4):  ref base (57/121):  total (62/125)
Fisher's exact test strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.05e-01

Only 100 of 193 total aligned reads displayed.

GGTGACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGC  >  NC_014034/914971‑915171
                                                                                                    |                                                                                                    
   gACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGc                                                                                                   <  2:2904550/101‑1 (MQ=255)
    aCCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCa                                                                                                  <  2:2983588/101‑1 (MQ=255)
       gACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGa                                                                                               <  2:1657485/101‑1 (MQ=255)
        gcgccgcCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGAt                                                                                              <  1:2968048/100‑1 (MQ=255)
         gaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCcaagggg                                                                                             >  1:4889394‑M1/45‑98 (MQ=255)
         cgccgcCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATa                                                                                             <  1:4868194/101‑1 (MQ=255)
          gccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaa                                                                                            <  1:3741172/101‑1 (MQ=255)
          cccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaa                                                                                            <  1:1442121/100‑1 (MQ=255)
          aaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGccaagggga                                                                                            >  1:877382‑M1/44‑97 (MQ=255)
          aaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGccaagggga                                                                                            <  1:2477186‑M1/58‑5 (MQ=255)
           ccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaaa                                                                                           <  1:4796932/101‑1 (MQ=255)
           accgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCgccaaggggaa                                                                                           <  2:4320394‑M1/59‑6 (MQ=255)
            cgccCGGGCCGCGGCGTAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaaaa                                                                                          <  2:3985408/101‑1 (MQ=255)
            cgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaaaa                                                                                          <  1:957855/101‑1 (MQ=255)
            cgccCGGCCCGCGTCGCAATCGCTTCTGTACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaaaa                                                                                          <  2:2478535/101‑1 (MQ=255)
            cccgcGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaaaa                                                                                          <  1:1621257/97‑1 (MQ=255)
             gccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAg                                                                                         <  1:3488300/101‑1 (MQ=255)
               ccGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAggg                                                                                       <  2:4074522/101‑1 (MQ=255)
                cGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGc                                                                                      <  2:1864151/101‑1 (MQ=255)
                 gggCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCa                                                                                     <  2:1245555/101‑1 (MQ=255)
                 gggCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCa                                                                                     <  2:10893/101‑1 (MQ=255)
                       cggcgCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCt                                                                               <  1:670278/101‑1 (MQ=255)
                         gcgcAATCGCTTCTGCACGGCTGAGGCAGGGGTGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGt                                                                             <  2:4576154/101‑1 (MQ=255)
                          cgcAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTc                                                                            <  2:1295214/101‑1 (MQ=255)
                          cgcAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTc                                                                            <  1:3092124/101‑1 (MQ=255)
                             aaTCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTcccc                                                                         <  2:2495822/101‑1 (MQ=255)
                             aaTCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTcccc                                                                         <  2:3133537/101‑1 (MQ=255)
                                cGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTccccccc                                                                      <  1:988561/101‑1 (MQ=255)
                                 gCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCt                                                                     <  2:2074980/101‑1 (MQ=255)
                                 gCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCt                                                                     <  2:1009540/101‑1 (MQ=255)
                                  cTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTg                                                                    <  2:8673/101‑1 (MQ=255)
                                    tCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGcc                                                                  <  1:3182401/101‑1 (MQ=255)
                                    tCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGcc                                                                  <  2:3922929/101‑1 (MQ=255)
                                      tGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCtt                                                                <  1:3019636/101‑1 (MQ=255)
                                      tGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCtt                                                                <  2:1954345/101‑1 (MQ=255)
                                      tGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCtt                                                                <  2:308119/101‑1 (MQ=255)
                                       gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                               <  2:42651/101‑1 (MQ=255)
                                       gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                               <  2:591972/101‑1 (MQ=255)
                                       gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                               <  1:4505320/101‑1 (MQ=255)
                                       gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                               <  1:1046923/101‑1 (MQ=255)
                                        cACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAGGGGCAGGGGCTGTCCCCCCCTGCCTTAc                                                              <  2:3369883/101‑1 (MQ=255)
                                         aCGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACg                                                             <  2:3386917/101‑1 (MQ=255)
                                           ggCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGAt                                                           <  2:2173725/101‑1 (MQ=255)
                                              tGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTcc                                                        <  2:1566026/101‑1 (MQ=255)
                                                 ggCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacc                                                     <  2:3988495/101‑1 (MQ=255)
                                                  gCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacca                                                    <  1:2554030/101‑1 (MQ=255)
                                                  gCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacca                                                    <  2:2378425/101‑1 (MQ=255)
                                                  gCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacca                                                    <  1:358632/101‑1 (MQ=255)
                                                   cAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCcaccac                                                   <  1:3874183/101‑1 (MQ=255)
                                                    aGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACg                                                  <  1:2525651/101‑1 (MQ=255)
                                                      gggggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGaa                                                <  2:2950051/101‑1 (MQ=255)
                                                        gggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAAtt                                              >  2:3156535/1‑101 (MQ=255)
                                                        gggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAAtt                                              <  2:3841290/101‑1 (MQ=255)
                                                         ggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTg                                             <  1:2637111/101‑1 (MQ=255)
                                                           cgcTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTgcg                                           >  2:217159/1‑101 (MQ=255)
                                                           cgcTGCCCCCCGGCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTgcg                                           <  1:1954537/101‑1 (MQ=255)
                                                            gcTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTgcgc                                          >  1:2901917/1‑101 (MQ=255)
                                                             cTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGcgcg                                         >  2:4793643/1‑101 (MQ=255)
                                                              tGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCgg                                        <  1:203810/101‑1 (MQ=255)
                                                                ccccccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTg                                      <  1:3377245/101‑1 (MQ=255)
                                                                  ccccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGAc                                    <  2:4454057/101‑1 (MQ=255)
                                                                    ccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGa                                  >  2:52374/1‑101 (MQ=255)
                                                                     cttCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGAt                                 <  1:2876614/99‑1 (MQ=255)
                                                                       tCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGAtag                               >  2:2876401/1‑99 (MQ=255)
                                                                       tCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCt                               <  2:4387820/101‑1 (MQ=255)
                                                                       tCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCt                               >  2:4282725/1‑101 (MQ=255)
                                                                        cccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTg                              >  2:2588725/1‑101 (MQ=255)
                                                                        cccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTg                              >  1:1801560/1‑101 (MQ=255)
                                                                        cccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTg                              >  2:4925088/1‑101 (MQ=255)
                                                                        cccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTg                              >  1:4433215/1‑101 (MQ=255)
                                                                         ccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGc                             >  1:2105463/1‑101 (MQ=255)
                                                                          cTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGcc                            >  2:2800296/1‑101 (MQ=255)
                                                                           tGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGAcagatcggaa                           >  1:1637396/1‑92 (MQ=255)
                                                                             cGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAAt                         >  1:1216673/1‑101 (MQ=255)
                                                                              gggACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATc                        >  1:1354139/1‑101 (MQ=255)
                                                                                gACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCt                      >  1:3555065/1‑101 (MQ=255)
                                                                                 aCTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTc                     <  1:2235191/101‑1 (MQ=255)
                                                                                  cTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCg                    <  2:3026178/101‑1 (MQ=255)
                                                                                   tCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCgg                   <  1:724116/101‑1 (MQ=255)
                                                                                    ccccccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCggg                  <  1:33119/101‑1 (MQ=255)
                                                                                    ccccccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCggg                  <  2:1166739/101‑1 (MQ=255)
                                                                                    ccccccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCggg                  >  2:371976/1‑101 (MQ=255)
                                                                                          gggATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGTTTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAg            >  1:4176517/1‑101 (MQ=255)
                                                                                           ggATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAgg           >  1:3448456/1‑101 (MQ=255)
                                                                                            gATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGc          >  2:2228604/1‑101 (MQ=255)
                                                                                             atatTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCCAGGCg         >  1:1346784/1‑101 (MQ=255)
                                                                                              tatTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGt        >  2:909014/1‑101 (MQ=255)
                                                                                               atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt       <  1:366410/101‑1 (MQ=255)
                                                                                               atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt       >  2:1739135/1‑101 (MQ=255)
                                                                                                 ttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCg     <  1:567181/101‑1 (MQ=255)
                                                                                                 ttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCg     <  1:4978992/101‑1 (MQ=255)
                                                                                                 ttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCg     >  1:4139239/1‑101 (MQ=255)
                                                                                                 ttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCg     >  1:4165734/1‑101 (MQ=255)
                                                                                                  tCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGa    >  1:1397034/1‑101 (MQ=255)
                                                                                                  tCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGa    <  1:3036849/101‑1 (MQ=255)
                                                                                                   cTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAg   >  1:1052623/1‑101 (MQ=255)
                                                                                                   cTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAg   <  2:1801427/101‑1 (MQ=255)
                                                                                                   cTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAg   >  1:2609598/1‑101 (MQ=255)
                                                                                                    tGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGc  <  2:3046631/101‑1 (MQ=255)
                                                                                                    tGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGc  >  1:4753421/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GGTGACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGC  >  NC_014034/914971‑915171

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG