Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,733,287 2 bp→GC 100% coding (309‑310/1128 nt) tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,733,2870C→G100.0% 748.4 203S103S (TCC→TCGtgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (104/99):  ref base (0/0):  total (104/99)
*NC_0140341,733,2880G→C100.0% 752.2 203M104M (GTG→CTG) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (104/99):  ref base (0/0):  total (104/99)

Only 100 of 226 total aligned reads displayed.

CGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAAGC  >  NC_014034/1733192‑1733384
                                                                                               ||                                                                                                
cgcATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTgcg                                                                                              >  1:4121834/1‑101 (MQ=255)
 gcATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTgcgc                                                                                             >  1:3157045/1‑101 (MQ=255)
  cATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCa                                                                                            <  2:1978285/101‑1 (MQ=255)
       cgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTg                                                                                       <  1:3757883/101‑1 (MQ=255)
        gcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGa                                                                                      <  2:364917/101‑1 (MQ=255)
         cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                     >  2:234693/1‑101 (MQ=255)
          ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                    <  1:1162900/101‑1 (MQ=255)
           gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                   >  1:1707080/1‑101 (MQ=255)
           gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                   >  2:1227398/1‑101 (MQ=255)
             tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                                 >  2:2750834/1‑101 (MQ=255)
             tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                                 >  2:3484511/1‑101 (MQ=255)
             tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                                 >  2:406957/1‑101 (MQ=255)
              gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                                >  1:352828/1‑101 (MQ=255)
               ggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagga                                                                               <  1:4990708/101‑1 (MQ=255)
                gCGGGGTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                              <  2:776779/101‑1 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                             >  2:247914/1‑101 (MQ=255)
                    gTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCg                                                                          >  2:2655616/1‑101 (MQ=255)
                    gTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCg                                                                          >  2:54023/1‑101 (MQ=255)
                      tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                        <  1:234727/101‑1 (MQ=255)
                      tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                        >  1:3277137/1‑101 (MQ=255)
                        cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                      >  2:2272087/1‑101 (MQ=255)
                        cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTCAc                                                                      <  1:2888844/101‑1 (MQ=255)
                         acaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAcc                                                                     <  1:1103147/101‑1 (MQ=255)
                          caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                    <  2:4760143/101‑1 (MQ=255)
                           aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                   <  1:221353/101‑1 (MQ=255)
                           aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                   <  1:829561/101‑1 (MQ=255)
                           aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                   >  1:2662452/1‑101 (MQ=255)
                           aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                   >  2:1623676/1‑101 (MQ=255)
                            aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                  >  1:3179350/1‑101 (MQ=255)
                            aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                  <  2:4892038/101‑1 (MQ=255)
                            aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                  >  1:402156/1‑101 (MQ=255)
                            aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                  <  2:4699934/101‑1 (MQ=255)
                            aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                  >  2:1593856/1‑101 (MQ=255)
                             gTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtttt                                                                 >  2:923927/1‑101 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                                <  1:2998969/101‑1 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                                <  2:1393394/101‑1 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                                <  1:4620128/101‑1 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGGTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGGGGGCGTGACCTTTTc                                                                >  2:2068846/1‑101 (MQ=255)
                                cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                              >  2:4357086/1‑101 (MQ=255)
                                cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                              >  1:4761181/1‑101 (MQ=255)
                                    aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                          <  2:2025461/101‑1 (MQ=255)
                                      cTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCata                                                        >  1:1595334/1‑101 (MQ=255)
                                       tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                       >  2:4020799/1‑101 (MQ=255)
                                       tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                       <  2:4653881/101‑1 (MQ=255)
                                       tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                       <  1:87044/101‑1 (MQ=255)
                                       tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                       <  1:1026335/101‑1 (MQ=255)
                                        gggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATc                                                      <  2:885710/101‑1 (MQ=255)
                                        gggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATc                                                      >  2:4139697/1‑101 (MQ=255)
                                         ggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCg                                                     <  1:1061845/101‑1 (MQ=255)
                                           aGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGAc                                                   <  2:3141162/101‑1 (MQ=255)
                                            gcgcCGGATCCTGACCGACTCGGGCGGGTTTCAGGTAATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                  <  2:385637/101‑1 (MQ=255)
                                              gcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGc                                                >  2:2244768/1‑101 (MQ=255)
                                                ccGATCCTGACCGACTCGGGGGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTc                                              <  1:2845557/101‑1 (MQ=255)
                                                ccGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTc                                              >  1:2571459/1‑101 (MQ=255)
                                                 cGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                             >  1:1354047/1‑101 (MQ=255)
                                                 cGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                             <  2:4187160/101‑1 (MQ=255)
                                                 cGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                             <  2:3189846/101‑1 (MQ=255)
                                                 cGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                             <  1:3484776/101‑1 (MQ=255)
                                                   aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaa                                           >  2:3354542/1‑101 (MQ=255)
                                                    tCCTGACCGACTCGGGCTGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaac                                          >  1:2195266/1‑100 (MQ=255)
                                                     ccTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                         >  2:16923/1‑101 (MQ=255)
                                                      cTGCCCGCCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAaca                                        <  1:3541537/101‑1 (MQ=255)
                                                      cTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAaca                                        >  1:1208393/1‑101 (MQ=255)
                                                      cTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAaca                                        >  2:405335/1‑101 (MQ=255)
                                                       tGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                       >  2:3950403/1‑101 (MQ=255)
                                                       tGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                       <  1:407022/101‑1 (MQ=255)
                                                        gACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACAcc                                      >  1:4439143/1‑101 (MQ=255)
                                                        gACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACAcc                                      <  2:3062589/101‑1 (MQ=255)
                                                         aCCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCa                                     >  2:396929/1‑101 (MQ=255)
                                                          gcGAATCGGGCGGGTTTGAGGGGATGAGCCTTGCTTCGCTGCGCAAGCTGCCCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                    <  2:880909/100‑1 (MQ=255)
                                                          ccGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                    <  1:4085169/101‑1 (MQ=255)
                                                           cGACTCGGGCGGGTTTCAGGTGATTAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATc                                   <  2:4600059/101‑1 (MQ=255)
                                                            gccTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCt                                  <  1:2272242/99‑1 (MQ=255)
                                                            gacgggggggggTTTCAGGTGATGCGCCTTGCTTCGGGGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCt                                  <  2:1755612/96‑1 (MQ=255)
                                                            gACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACAACATCt                                  >  1:744233/1‑101 (MQ=255)
                                                             aCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAAAACCATCTg                                 >  2:2124400/1‑101 (MQ=255)
                                                               tggggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                               <  2:4438972/99‑1 (MQ=255)
                                                                    cgggTTTCAGGTGATGAGCCTTGCTTCGCGGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCg                          <  2:1072207/101‑1 (MQ=255)
                                                                     gggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCTTATCGACGGCTCGAAACACCATCTGTCGCCCGa                         >  1:4356805/1‑101 (MQ=255)
                                                                      ggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaa                        >  1:1288080/1‑101 (MQ=255)
                                                                        tttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACg                      >  1:4510459/1‑101 (MQ=255)
                                                                        tttCAGGTGATGAGCCTTGCTTCGCCGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACg                      <  2:829878/101‑1 (MQ=255)
                                                                         ttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGc                     >  1:2783125/1‑101 (MQ=255)
                                                                          tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCt                    >  2:4550896/1‑101 (MQ=255)
                                                                           cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                   <  1:1914213/101‑1 (MQ=255)
                                                                            aGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCg                  <  2:2299125/101‑1 (MQ=255)
                                                                            aGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCg                  >  2:828143/1‑101 (MQ=255)
                                                                             ggTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGa                 >  1:1756755/1‑101 (MQ=255)
                                                                             ggTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGa                 <  2:3460807/101‑1 (MQ=255)
                                                                             ggTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGa                 <  2:2554032/101‑1 (MQ=255)
                                                                                gatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATAGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg              <  1:2376908/101‑1 (MQ=255)
                                                                                gatgaGCCTTGCTCCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg              <  1:449605/101‑1 (MQ=255)
                                                                                 atgaGCCTTGCTTCGCTGCGCAAGCTGACGGAGGGGGGGGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgta             <  1:3425156/101‑3 (MQ=255)
                                                                                 atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga             >  1:3225904/1‑101 (MQ=255)
                                                                                  tgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgag            >  1:4592930/1‑101 (MQ=255)
                                                                                  tgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgag            >  2:1493928/1‑101 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGGGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa       <  1:1499648/101‑1 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa       <  1:3739601/101‑1 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa       >  1:3460197/1‑101 (MQ=255)
                                                                                         ggCTTCCCGGCGCAAGGTGCCCGAGGGGGGCGTGACCTTTCCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa     <  1:2022859/100‑1 (MQ=255)
                                                                                               ||                                                                                                
CGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAAGC  >  NC_014034/1733192‑1733384

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG