Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,751,825 2 bp→GC 100% intergenic (+154/‑203) RCAP_rcc01619 → / → aldB Fis family transcriptional regulator/aldehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,751,8250C→G100.0% 759.4 211intergenic (+154/‑204)RCAP_rcc01619/aldBFis family transcriptional regulator/aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (150/61):  ref base (0/0):  total (150/61)
*NC_0140341,751,8260G→C100.0% 756.8 211intergenic (+155/‑203)RCAP_rcc01619/aldBFis family transcriptional regulator/aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (150/61):  ref base (0/0):  total (150/61)

Only 100 of 220 total aligned reads displayed.

GGATATTTGAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCACGCGAGGGGGGCAGA.G.CCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGA  >  NC_014034/1751725‑1751917
                                                                                                    ||                                                                                               
     tttGAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAg                                                                                             >  1:4766862/1‑101 (MQ=255)
      ttGAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAgg                                                                                            <  2:4079199/101‑1 (MQ=255)
       tGAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAggg                                                                                           >  2:3723928/1‑101 (MQ=255)
        gAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAgggg                                                                                          >  2:4733826/1‑101 (MQ=255)
        gAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAgggg                                                                                          <  1:1331762/101‑1 (MQ=255)
        gAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAgggg                                                                                          >  1:3183841/1‑101 (MQ=255)
        gAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAgggg                                                                                          >  2:3375980/1‑101 (MQ=255)
         aaGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAggggg                                                                                         >  1:4007632/1‑101 (MQ=255)
           gCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGc                                                                                       >  1:1514673/1‑101 (MQ=255)
           gCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGc                                                                                       >  2:3258573/1‑101 (MQ=255)
            cAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCa                                                                                      >  1:4724321/1‑101 (MQ=255)
            cAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCa                                                                                      >  2:4419812/1‑101 (MQ=255)
            cAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCa                                                                                      >  1:4642693/1‑101 (MQ=255)
             aaGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCag                                                                                     >  2:1346374/1‑101 (MQ=255)
             aaGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCag                                                                                     >  2:3856869/1‑101 (MQ=255)
             aaGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCag                                                                                     >  2:4137771/1‑101 (MQ=255)
                aTAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.c                                                                                <  1:1681508/101‑1 (MQ=255)
                 tAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc                                                                               >  2:4473127/1‑101 (MQ=255)
                  aaaGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccc                                                                              >  1:4835428/1‑101 (MQ=255)
                   aaGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cccc                                                                             >  1:3563243/1‑101 (MQ=255)
                   aaGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cccc                                                                             >  1:3942956/1‑101 (MQ=255)
                     ggATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cccccc                                                                           >  1:1105552/1‑100 (MQ=255)
                     ggATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cccccc                                                                           >  1:1001239/1‑100 (MQ=255)
                      gATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cccccca                                                                          <  2:233101/101‑3 (MQ=255)
                        tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccaga                                                                        >  1:813729/1‑97 (MQ=255)
                        tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccaga                                                                        >  2:1404511/1‑97 (MQ=255)
                        tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccaga                                                                        >  1:4736980/1‑97 (MQ=255)
                        tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccaga                                                                        >  1:2397533/1‑97 (MQ=255)
                        tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccaga                                                                        >  1:3644019/1‑97 (MQ=255)
                         aaGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGACCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.ccccccagac                                                                       >  2:828902/1‑96 (MQ=38)
                          aGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.Acc                                                                     >  1:1615150/1‑101 (MQ=37)
                          aGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.Acc                                                                     >  1:4947078/1‑101 (MQ=37)
                           ggCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCCCCTTGCCCCAAATATCCCGGGGGGGGTGGCGCAGCCGAGGGGGGCCGA.G.ccccccacaacc                                                                     >  1:3442877/1‑94 (MQ=37)
                            gcgcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.Acccc                                                                   <  1:1955439/101‑1 (MQ=37)
                            gcgcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.Acccc                                                                   <  2:1252348/101‑1 (MQ=37)
                             cgcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCCCCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCg                                                                  <  1:2202191/101‑1 (MQ=37)
                             cgcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCg                                                                  <  1:2230019/101‑1 (MQ=37)
                              gcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGc                                                                 >  2:4821311/1‑101 (MQ=38)
                              gcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGc                                                                 <  1:2804514/101‑1 (MQ=37)
                               cTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGcc                                                                >  2:3796784/1‑101 (MQ=38)
                               cTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGcc                                                                >  2:546354/1‑101 (MQ=38)
                                tCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCt                                                               <  2:1088652/101‑1 (MQ=37)
                                 ccGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTg                                                              >  2:3120918/1‑101 (MQ=38)
                                 ccGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.CCCCC.AGAACGCCGCCTg                                                              >  2:179856/1‑101 (MQ=37)
                                  cGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGc                                                             >  2:70538/1‑101 (MQ=38)
                                   gCAAGGGCCCCTTCTCCCATTCCCGCCTTTCACCTTGCCCCAAATATCCCAGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCg                                                            <  2:1726781/101‑1 (MQ=25)
                                     aaGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGAc                                                          >  1:2744638/1‑101 (MQ=38)
                                     aaGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGAc                                                          >  2:2617609/1‑101 (MQ=38)
                                     aaGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGAc                                                          >  2:676790/1‑101 (MQ=38)
                                         gcgcCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCcg                                                      >  1:2440316/1‑101 (MQ=38)
                                          cgcCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCcgc                                                     <  1:2830979/101‑1 (MQ=37)
                                             cttcttCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGAGGTGAGGCGCAGCCGAGGGGGGGTGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGcgcc                                                  >  1:4317134/1‑101 (MQ=37)
                                              ttcttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGcgccg                                                 >  1:3858778/1‑101 (MQ=38)
                                               tcttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGcgccgc                                                >  2:3387856/1‑101 (MQ=38)
                                                cttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGACA.CCCCC.AG.ACCCCGCCTGCGACCCCGCgccgcc                                               >  1:4377404/1‑101 (MQ=38)
                                                 ttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCt                                              >  2:570628/1‑101 (MQ=38)
                                                  tcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCtt                                             <  1:3239219/101‑1 (MQ=37)
                                                  tcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCtt                                             >  1:4455031/1‑101 (MQ=38)
                                                   ccATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCttt                                            >  1:4575813/1‑101 (MQ=38)
                                                   ccATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCttt                                            >  1:553625/1‑101 (MQ=38)
                                                    cATTTCCGCCTTTCACCTTGCCCCAAATATCCGGGGGGGTGAGGCGCAGCGGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTa                                           <  2:1792076/101‑1 (MQ=37)
                                                    cATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTa                                           >  1:559402/1‑101 (MQ=38)
                                                     aTTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTAc                                          >  2:2242621/1‑101 (MQ=38)
                                                      tttCCGCCTTTCACCTTGCCCCAAATATCCGGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACg                                         <  1:1255272/101‑1 (MQ=37)
                                                      tttCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACg                                         <  1:3000682/101‑1 (MQ=37)
                                                      tttCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACg                                         <  1:3702591/101‑1 (MQ=37)
                                                        tCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGaa                                       >  2:365621/1‑101 (MQ=38)
                                                          cGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAAtt                                     <  2:1164638/101‑1 (MQ=37)
                                                               ttCACCTTGCCCCAAATATCCTGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCg                                >  1:2873862/1‑101 (MQ=37)
                                                               ttCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCg                                >  2:106730/1‑101 (MQ=37)
                                                                tCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGa                               >  2:2177861/1‑101 (MQ=37)
                                                                 cACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAt                              >  2:2676631/1‑101 (MQ=37)
                                                                  cccTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtg                             <  2:3576899/100‑1 (MQ=37)
                                                                   ccTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgc                            >  1:869415/1‑101 (MQ=38)
                                                                     ttGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctg                          >  2:1227739/1‑101 (MQ=38)
                                                                      tGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctgc                         >  1:460045/1‑101 (MQ=38)
                                                                       gCCCCAAGTATCGGGGGGGGTGAGCGGCGGCCGAGGGGGGCAGA.GCCCCCC.AG.CCCCCGCCTGCGCCCCCGCGCCGCCTTTACGAATTTTGCGATgctgca                        <  1:203765/101‑1 (MQ=255)
                                                                         cgcAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGTCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagc                      <  2:3352997/99‑1 (MQ=37)
                                                                          ccAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagct                     >  1:3327915/1‑101 (MQ=38)
                                                                            aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                   >  2:2896147/1‑101 (MQ=38)
                                                                            aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                   >  1:4705583/1‑101 (MQ=38)
                                                                            aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                   >  2:4266617/1‑101 (MQ=38)
                                                                             aaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgca                  >  1:3898300/1‑101 (MQ=38)
                                                                               tatCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGAAcc                >  1:3037626/1‑101 (MQ=38)
                                                                                atCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCt               >  2:1663828/1‑101 (MQ=38)
                                                                                  cccGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGt             >  1:3538692/1‑101 (MQ=39)
                                                                                   ccGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtc            >  1:2622441/1‑101 (MQ=39)
                                                                                    gggggggTGAGGCGCAGCCGGGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtct           <  1:944575/100‑1 (MQ=255)
                                                                                    cGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtct           >  2:2211015/1‑101 (MQ=255)
                                                                                    cGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtct           <  2:2753422/101‑1 (MQ=255)
                                                                                     ggggggTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtctc          <  2:1848323/101‑1 (MQ=255)
                                                                                       ggggTGAGGCGCAGCCGGGGGGGGCAGA.G.CCCCCGAG.ACCCCGCCTGCGCCCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCgg        <  2:4686522/101‑1 (MQ=255)
                                                                                       ggggTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCgg        <  2:127221/101‑1 (MQ=255)
                                                                                        gggTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGc       >  2:1514065/1‑101 (MQ=255)
                                                                                          gTGAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAg     >  1:2422726/1‑101 (MQ=255)
                                                                                            gAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTg   >  2:3460290/1‑101 (MQ=255)
                                                                                            gAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTg   >  2:4873840/1‑101 (MQ=255)
                                                                                            gAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTg   >  2:1802528/1‑101 (MQ=255)
                                                                                            gAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTg   <  1:1159991/101‑1 (MQ=255)
                                                                                            gAGGCGCAGCCGAGGGGGGCAGA.GCCCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCCGTCTCGGCAGTg   >  2:4962297/1‑101 (MQ=255)
                                                                                                    ||                                                                                               
GGATATTTGAAGCAAGATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCACGCGAGGGGGGCAGA.G.CCCCC.AG.ACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGA  >  NC_014034/1751725‑1751917

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG