Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,243 2 bp→GC 100% coding (626‑627/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2430C→G100.0% 841.3 232T209R (ACG→AGG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (109/123):  ref base (0/0):  total (109/123)
*NC_0140341,802,2440G→C100.0% 865.6 232T209T (ACG→ACCligADNA ligase
Reads supporting (aligned to +/- strand):  new base (108/124):  ref base (0/0):  total (108/124)

Only 100 of 242 total aligned reads displayed.

GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338
                                                                                               ||                                                                                              
ggCGCCTGGCGTGCTGGAAGTCCGCGGCGAGGTCTAGATGAGCCAGGCCGATTTTGCGGCGCGGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCg                                                                                            <  2:3115150/101‑1 (MQ=255)
ggCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCg                                                                                            <  2:2490244/101‑1 (MQ=255)
 ccccatGCCGTGCTGGAAGTCCGCGGCGACCTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCTGCAGGCGGAGCGGGGCGACAAGAGCTTCGc                                                                                           <  2:4507795/96‑1 (MQ=255)
    ccTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCaa                                                                                        <  1:3543956/101‑1 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       <  2:3239123/101‑1 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       >  1:282101/1‑101 (MQ=255)
      tGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATc                                                                                      >  2:4029761/1‑101 (MQ=255)
       gCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATcc                                                                                     >  1:5009404/1‑101 (MQ=255)
       gCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATcc                                                                                     <  2:3192967/101‑1 (MQ=255)
       gCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATcc                                                                                     <  1:421555/101‑1 (MQ=255)
         cGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgc                                                                                   >  1:3204403/1‑101 (MQ=255)
           tGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgc                                                                                 >  1:3493753/1‑101 (MQ=255)
            gCTGGAAGTCCGCGGGGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCa                                                                                <  2:632658/101‑1 (MQ=255)
            gCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCa                                                                                <  2:4076516/101‑1 (MQ=255)
             cTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgcca                                                                               >  2:500824/1‑99 (MQ=255)
             cTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCaa                                                                               >  1:2908489/1‑101 (MQ=255)
             cTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCaa                                                                               >  2:3615580/1‑101 (MQ=255)
               ggAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAta                                                                             >  1:3969387/1‑100 (MQ=255)
               ggAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATg                                                                             >  1:755084/1‑101 (MQ=255)
               ggAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATg                                                                             >  2:1387416/1‑101 (MQ=255)
                gAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgc                                                                            <  2:3161872/101‑1 (MQ=255)
                 aaGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgcc                                                                           <  2:3529651/101‑1 (MQ=255)
                  aGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccg                                                                          <  1:581054/101‑1 (MQ=255)
                   gTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgc                                                                         >  2:1313268/1‑101 (MQ=255)
                      cgcgGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCcgccgc                                                                      >  2:4188880/1‑101 (MQ=255)
                           cgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCt                                                                 >  1:4282265/1‑101 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATTCGCGCAATGCCGCCGCGGGCTc                                                                <  1:886024/101‑1 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                >  2:3818171/1‑101 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               >  2:3952707/1‑100 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               >  2:5045357/1‑100 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               >  2:4629946/1‑100 (MQ=255)
                               gTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgct                                                             >  1:2115315/1‑98 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            >  2:1499747/1‑97 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           >  2:2723201/1‑96 (MQ=255)
                                   aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                         >  2:4430750/1‑101 (MQ=255)
                                     aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCNGCGCAATGCCGCCGCGGGCTCCCTGCGCCa                                                       >  1:1002564/1‑101 (MQ=255)
                                     aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                       >  1:2006499/1‑101 (MQ=255)
                                     aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                       <  2:857891/101‑1 (MQ=255)
                                       gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                     <  1:3270519/101‑1 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    >  2:3927515/1‑101 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTAGCCAATCCGCGCAATGCCGCCGCGGGCTCCCTGCGCCAGcc                                                    >  1:1520467/1‑100 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   <  1:3106371/101‑1 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   <  1:3881615/101‑1 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGa                                                 <  2:1017934/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  2:30166/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGAAATGCCGCCGCGTGCTCGCTGCGCCAGCTCGAc                                                <  2:1671511/101‑1 (MQ=255)
                                              gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                              <  1:132879/101‑1 (MQ=255)
                                                 gATTTTGCGGCGCTTAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                           <  1:4355871/101‑1 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                          >  1:1070779/1‑101 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        >  2:4820872/1‑101 (MQ=255)
                                                     ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                       <  2:905037/101‑1 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     <  2:3394266/101‑1 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    <  1:1349840/101‑1 (MQ=255)
                                                         ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                   <  1:3322121/101‑1 (MQ=255)
                                                          gcgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGc                                  >  2:2105871/1‑101 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGGGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 <  1:4893057/101‑1 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                <  1:621476/101‑1 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                >  2:1884804/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGGGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              <  1:2343799/101‑1 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:2002360/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:2593074/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:3818456/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             >  2:60524/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:2433918/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:978002/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            >  1:2567321/1‑101 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:1703918/101‑1 (MQ=255)
                                                                 aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAgc                           >  1:1902201/1‑100 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         <  2:3904861/101‑1 (MQ=255)
                                                                    cgcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAccc                        <  1:3416126/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  1:4474011/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  2:2226787/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:727839/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     >  1:3461975/1‑101 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  2:1341581/101‑1 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  >  1:3358534/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 >  2:1872703/1‑101 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              >  1:4517333/1‑101 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              >  1:1170879/1‑101 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             <  2:3745356/101‑1 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            <  1:47720/101‑1 (MQ=255)
                                                                                 gCGGGGGGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg           <  1:2757896/101‑1 (MQ=255)
                                                                                 gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg           <  1:1459443/101‑1 (MQ=255)
                                                                                  cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc          <  1:3989194/101‑1 (MQ=255)
                                                                                  cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc          >  2:2402602/1‑101 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         >  2:2695408/1‑101 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        <  2:2810000/101‑1 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        <  2:4333407/101‑1 (MQ=255)
                                                                                     ggCGACAAGATCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa       <  2:1241777/101‑1 (MQ=255)
                                                                                     ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa       >  1:2217959/1‑101 (MQ=255)
                                                                                     ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa       <  2:4971006/101‑1 (MQ=255)
                                                                                      gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACAGCCTCCAGACCCTTGAAATTCTTTGCCTAt      >  2:4719049/1‑101 (MQ=255)
                                                                                      gCGACAAGAGCTTCGCCAATCCGCGCAATACCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt      <  2:2843065/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  2:3443816/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  1:1845893/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  1:4630269/101‑1 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    >  2:3643921/1‑101 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    <  1:3847298/101‑1 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   >  1:1401256/1‑101 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc   >  2:2655394/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG