Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,053,078 2 bp→GC 100% coding (4156‑4157/6198 nt) RCAP_rcc01900 ← hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,053,0780C→G100.0% 729.2 198R1386P (CGG→CCG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (96/102):  ref base (0/0):  total (96/102)
*NC_0140342,053,0790G→C100.0% 736.9 198R1386G (CGG→GGG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (96/102):  ref base (0/0):  total (96/102)

Only 100 of 216 total aligned reads displayed.

GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052983‑2053173
                                                                                               ||                                                                                              
  gtttCGTTGCCCGCGCCGCCAAGGATGGTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGcc                                                                                          <  2:4773648/98‑1 (MQ=38)
  aTGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGcc                                                                                          <  2:1775087/101‑1 (MQ=255)
   tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                         <  2:4989436/101‑1 (MQ=255)
    gTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGa                                                                                        >  1:1631499/1‑101 (MQ=255)
    gTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGa                                                                                        >  2:2281736/1‑101 (MQ=255)
     ccGTGGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAg                                                                                       <  2:4698401/100‑1 (MQ=255)
      cGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAgg                                                                                      <  1:1343876/101‑1 (MQ=255)
      cGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAgg                                                                                      <  1:2996058/101‑1 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGa                                                                                     >  1:3279047/1‑101 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGa                                                                                     >  1:325749/1‑101 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGa                                                                                     >  1:3213830/1‑101 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                  >  1:1603936/1‑101 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                  >  1:279871/1‑101 (MQ=255)
             cgcgCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtgt                                                                               >  2:2019390/1‑101 (MQ=255)
              gcgcCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTc                                                                              <  1:2851038/101‑1 (MQ=255)
               cgccgcCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATTGCCCGCTTCGCCGAGGATGGTGTCg                                                                             >  1:2953958/1‑101 (MQ=255)
                 ccgccAAGGATGCTGTCTTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  2:4859496/101‑1 (MQ=255)
                 ccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  1:2256906/101‑1 (MQ=255)
                  cgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTg                                                                          <  1:1011954/101‑1 (MQ=255)
                  cgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTg                                                                          >  2:620287/1‑101 (MQ=255)
                   gccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGc                                                                         <  2:2981087/101‑1 (MQ=255)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                       >  1:3720204/1‑101 (MQ=255)
                      aaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCt                                                                      >  1:4910963/1‑101 (MQ=255)
                        ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                    <  2:3570313/101‑1 (MQ=255)
                         gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCGCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   <  1:4172169/101‑1 (MQ=255)
                         gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   <  1:500183/101‑1 (MQ=255)
                         gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   >  2:1437767/1‑101 (MQ=255)
                          gtgcggccctcACCCCGCCCGCCGGTGATGCTGTCATCCCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                  <  2:3213579/92‑1 (MQ=21)
                          aTGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                  <  1:2772645/101‑1 (MQ=255)
                          aTGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcaga                                                                  >  2:1641807/1‑98 (MQ=255)
                            gCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGc                                                                >  1:1832205/1‑101 (MQ=255)
                            gCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGc                                                                <  1:1029518/101‑1 (MQ=255)
                              tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  1:800729/101‑1 (MQ=255)
                              tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  1:2320470/101‑1 (MQ=255)
                              cgtcctcATCCAGCCCGCCGGTGATGCTGTCATTCCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  1:478129/100‑1 (MQ=37)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  1:775737/1‑101 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  1:227163/1‑101 (MQ=255)
                                 cgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGc                                                           >  1:2956276/1‑101 (MQ=255)
                                 cgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGc                                                           >  2:3322467/1‑101 (MQ=255)
                                    ctcccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                        <  2:4960611/98‑1 (MQ=255)
                                    cATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                        <  1:3772947/101‑1 (MQ=255)
                                     aTCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCg                                                       >  2:2216490/1‑101 (MQ=255)
                                     aTCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCg                                                       <  2:199190/101‑1 (MQ=255)
                                     aTCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCg                                                       >  1:4740236/1‑101 (MQ=255)
                                        cAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGt                                                    <  1:4515260/101‑1 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   >  1:4879296/1‑101 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   <  1:3811633/101‑1 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   <  2:3027572/101‑1 (MQ=255)
                                            ccgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATc                                                <  2:2895225/101‑1 (MQ=255)
                                            ccgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATc                                                <  1:725543/101‑1 (MQ=255)
                                             cgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCg                                               <  1:4979693/101‑1 (MQ=255)
                                               gcgGTGATGCTGTGATTGCCCGCGCCGTCGTCCAGCTTGTCATCGCCCGCTTCGCCGAGCATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGcc                                             <  2:1631367/100‑1 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           >  1:3612346/1‑101 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           <  2:4357273/101‑1 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           <  2:1951924/101‑1 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                           <  2:3719926/101‑1 (MQ=255)
                                                  gTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGa                                          >  1:1966609/1‑101 (MQ=255)
                                                    gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                        >  1:1071997/1‑101 (MQ=255)
                                                      tGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGccc                                      <  1:409251/101‑1 (MQ=255)
                                                        cTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGCGGATGGTGTCGTTGCCCTCCCGGCCCAGCACCGAGTCCTCGCCCGAGCCCGc                                    >  1:1033260/1‑101 (MQ=37)
                                                         tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   >  1:4530429/1‑101 (MQ=255)
                                                         tGTCATTGCCCGCCCCCCCGTCCAGCGTGTGATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   <  2:105554/101‑1 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCTCCCGAGCCCGCCt                                  >  1:4341953/1‑101 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                  >  2:1559804/1‑101 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGACCGCCt                                  >  2:4420340/1‑101 (MQ=255)
                                                           tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                 <  2:3085461/101‑1 (MQ=255)
                                                           tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                 >  1:3994121/1‑101 (MQ=255)
                                                              ttGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgat                              >  2:4195397/1‑101 (MQ=255)
                                                               tGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                             <  1:522283/101‑1 (MQ=255)
                                                               tGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                             >  2:2414718/1‑101 (MQ=255)
                                                                 cccGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                           <  1:1295984/101‑1 (MQ=38)
                                                                  ccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGaa                          >  1:4082927/1‑101 (MQ=255)
                                                                    gcgcCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                        <  1:413006/101‑1 (MQ=39)
                                                                    gcgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                        <  2:4455030/101‑1 (MQ=255)
                                                                     cgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcg                       >  2:3619530/1‑101 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                      >  2:504817/1‑101 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                      >  1:4394699/1‑101 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgc                      >  2:3960588/1‑100 (MQ=255)
                                                                       ccACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtc                     <  2:2956048/101‑1 (MQ=255)
                                                                        cACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                    >  1:236905/1‑101 (MQ=255)
                                                                        cACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                    >  2:695760/1‑101 (MQ=255)
                                                                          ccGTCCAGCTTGTCATCGCCCGCTTCGTCTAGGTTGTGTTCGGTTCCTTCCCCGCCCAGCACCGAGTCATCCCCCTACCCCGCCTCGATCGAAACGTCGcc                  <  2:2502099/101‑1 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCCCCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGcc                  <  1:4196031/101‑1 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGcc                  <  2:1619179/101‑1 (MQ=255)
                                                                           cttCCAGCGTGTCATCCCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCg                 <  2:2814183/99‑1 (MQ=255)
                                                                             tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               <  1:994863/101‑1 (MQ=255)
                                                                             ccccgCGTGTCATCGCCCGCTGCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               <  1:2360585/97‑1 (MQ=255)
                                                                              ccggCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTg              <  1:141577/98‑1 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGc             <  1:2281900/101‑1 (MQ=255)
                                                                               cAGCGTGTCACCGCCCGCTTCGCAAAGGATAGTGTCGTTGCCCTCCCCGCCCAGCTCCGAGTCATCGCCTGACCCCGCCTCGATCGAATCGTCGCCGGTGc             <  1:4859989/101‑1 (MQ=255)
                                                                                  cGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcg          >  1:87520/1‑101 (MQ=255)
                                                                                  cGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTCCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcg          <  2:4311239/101‑1 (MQ=255)
                                                                                     gtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGt       >  1:1565016/1‑101 (MQ=255)
                                                                                      tCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCTAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGtt      >  2:1181035/1‑101 (MQ=255)
                                                                                      tCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCACGtt      >  2:3642080/1‑101 (MQ=255)
                                                                                       caccGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc     <  1:1698221/98‑1 (MQ=255)
                                                                                       cATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGGTc     >  2:4257301/1‑101 (MQ=255)
                                                                                       cATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc     <  2:775635/101‑1 (MQ=255)
                                                                                         cccccccCTTCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAc   <  2:4134334/98‑1 (MQ=255)
                                                                                          cGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACg  >  2:4087856/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052983‑2053173

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG