Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,900 2 bp→CA 100% coding (2674‑2675/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9000A→C100.0% 620.6 181V892G (GTA→GGA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (92/89):  ref base (0/0):  total (92/89)
*NC_0140342,304,9010C→A100.0% 616.4 181V892L (GTA→TTA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (91/89):  ref base (1/0):  total (92/89)

Only 100 of 186 total aligned reads displayed.

GATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATG.C.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304808‑2304988
                                                                                               ||                                                                                        
gATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCCGCTccc                                                                                    >  2:2893587/1‑101 (MQ=255)
 aTAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCg                                                                                   >  2:7146/1‑101 (MQ=255)
  tAATCTTGCGCAAACCGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGc                                                                                  <  2:819529/101‑1 (MQ=255)
  tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGc                                                                                  >  1:1097237/1‑101 (MQ=255)
   aaTCTTCCGCAAAACTGGGCCGGATCCGGCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCa                                                                                 <  1:2088995/101‑1 (MQ=255)
      ctggcaaaaaaCTCGGCCGGATCGGGCGTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.ggg                                                                             <  2:1424661/94‑2 (MQ=255)
       ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggc                                                                            >  1:4776095/1‑99 (MQ=255)
        tGCGCAAAACTCGGCCGGATCCGGGCTGTCAGCCCGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                           >  2:159248/1‑98 (MQ=255)
        tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.ggggc                                                                           >  2:4853549/1‑98 (MQ=255)
         gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:3803548/101‑5 (MQ=255)
         gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:2183115/101‑5 (MQ=255)
         gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          >  1:2473089/1‑97 (MQ=255)
         gcgaAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:77489/101‑5 (MQ=255)
          cgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                         >  2:4285698/1‑96 (MQ=255)
           gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.ggggctgc                                                                        >  2:2567359/1‑95 (MQ=255)
           gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.ggggctgc                                                                        >  1:1998611/1‑95 (MQ=255)
           ccAAAGCTCGCCCGATTCCGGCCTGCCACCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                         <  2:2519334/100‑1 (MQ=255)
              aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGcc                                                                      >  1:1823300/1‑101 (MQ=255)
               aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     >  1:2523181/1‑101 (MQ=255)
               aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     >  1:3949382/1‑101 (MQ=255)
                aCTCGGCCGGTTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                    <  2:2629083/101‑1 (MQ=255)
                aCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                    >  2:2543504/1‑101 (MQ=255)
                 cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   <  1:3179931/101‑1 (MQ=255)
                 cTCGGCCGGACCGGCCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   <  2:4912025/101‑1 (MQ=255)
                 cTCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   <  1:977032/101‑1 (MQ=255)
                   gggCCGGATCGGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                 <  1:4986565/100‑1 (MQ=255)
                   cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGcc                                                                 >  2:3532872/1‑101 (MQ=255)
                    ggCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                <  1:868752/101‑1 (MQ=255)
                     gcgggACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  1:2056116/98‑1 (MQ=255)
                     gCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  2:4309164/101‑1 (MQ=255)
                        ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            >  2:706426/1‑101 (MQ=255)
                        ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            >  1:2784044/1‑101 (MQ=255)
                        ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            >  2:1966451/1‑101 (MQ=255)
                         gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  1:1524288/1‑101 (MQ=255)
                         gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  1:2438177/1‑101 (MQ=255)
                          acccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                          <  1:4277411/99‑1 (MQ=255)
                            cggccttggcAGGCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGGCCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        <  2:4011356/92‑1 (MQ=255)
                            ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        <  1:4115481/101‑1 (MQ=255)
                            ccGGCCTGCCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        <  1:3684678/101‑1 (MQ=255)
                               gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  1:2740276/101‑1 (MQ=255)
                                ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCTCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                    >  1:4370972/1‑101 (MQ=255)
                                  tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                  >  2:3322317/1‑101 (MQ=255)
                                   gTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCggc                                                 <  2:1286962/101‑3 (MQ=255)
                                   gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCC.GAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  1:4246878/101‑1 (MQ=255)
                                   gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 >  2:2753618/1‑101 (MQ=255)
                                   gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  1:624535/101‑1 (MQ=255)
                                    tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                <  2:4711541/101‑1 (MQ=255)
                                     cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               >  2:1232618/1‑101 (MQ=255)
                                        ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGGGCCTGCGTCGCGGc                                            >  2:3344873/1‑101 (MQ=255)
                                        ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                            >  1:3158477/1‑101 (MQ=255)
                                         cgcccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                           <  1:3482132/98‑1 (MQ=255)
                                         cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                           <  1:4709747/101‑1 (MQ=255)
                                         cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                           <  1:402868/101‑1 (MQ=255)
                                             cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                       <  1:4041239/101‑1 (MQ=255)
                                               gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     >  2:4435963/1‑101 (MQ=255)
                                               gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAACAGCGCCTGCGTCGCGGCCGCGttt                                     >  1:707899/1‑101 (MQ=255)
                                               gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCCCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCCttt                                     <  1:304956/101‑1 (MQ=255)
                                                ccGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGgttt                                    >  1:2879519/1‑101 (MQ=255)
                                                 cGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                   >  1:3268232/1‑101 (MQ=255)
                                                  gCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  >  2:4607544/1‑101 (MQ=255)
                                                  gCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  >  1:1692389/1‑101 (MQ=255)
                                                     aCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                               >  2:2732058/1‑101 (MQ=255)
                                                        gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  1:683096/101‑1 (MQ=255)
                                                        gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            >  2:3909909/1‑101 (MQ=37)
                                                        gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            >  1:4799363/1‑101 (MQ=37)
                                                         cggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCACGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           >  2:2574315/1‑101 (MQ=255)
                                                          ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  1:1005113/101‑1 (MQ=255)
                                                          ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  2:1702234/1‑101 (MQ=255)
                                                          ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  1:4902892/1‑101 (MQ=255)
                                                           gcgcCGGTGGCG.CCTGCAGG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                         <  2:1498221/101‑1 (MQ=25)
                                                            cgcCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  1:2413117/101‑1 (MQ=25)
                                                            cgcCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  1:1439524/101‑1 (MQ=25)
                                                              ccGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                      >  1:573408/1‑101 (MQ=25)
                                                              ccGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                      <  2:595284/101‑1 (MQ=25)
                                                               cGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:1380820/1‑101 (MQ=25)
                                                                ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:2852134/1‑101 (MQ=25)
                                                                ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  2:3092883/1‑101 (MQ=25)
                                                                ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  2:3288950/1‑101 (MQ=25)
                                                                  tGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  >  2:4240703/1‑101 (MQ=25)
                                                                  gttgcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  1:1797605/7‑101 (MQ=21)
                                                                   gggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  <  2:3408481/96‑1 (MQ=25)
                                                                    ggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 >  1:1330578/5‑101 (MQ=25)
                                                                    ggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  2:3949090/97‑1 (MQ=25)
                                                                    ggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:3124796/97‑1 (MQ=25)
                                                                     gcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  1:2243237/98‑1 (MQ=25)
                                                                     gcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  1:3193565/98‑1 (MQ=25)
                                                                       gccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              <  1:282353/100‑1 (MQ=25)
                                                                           gCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  1:1005533/1‑101 (MQ=21)
                                                                           gCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  2:3663920/1‑101 (MQ=21)
                                                                                gcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      >  2:2676707/4‑101 (MQ=21)
                                                                                gcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  2:2719885/98‑1 (MQ=25)
                                                                                 cggAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggggaac     >  2:2033939/3‑97 (MQ=21)
                                                                                   gAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   <  1:3910201/101‑1 (MQ=25)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  >  2:4716689/1‑101 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  2:2916737/101‑1 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  1:2574496/101‑1 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  2:4919456/101‑1 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  1:3072427/101‑1 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  >  2:2236410/1‑101 (MQ=21)
                                                                                    aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  >  1:1750586/1‑101 (MQ=21)
                                                                                               ||                                                                                        
GATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATG.C.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304808‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG