STRAIN: SE8E

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8-E_1_sequence.txt5,054,872510,542,072100.0%101.0 bases101 bases92.7%
errorsSE8-E_2_sequence.txt5,054,542510,508,742100.0%101.0 bases101 bases91.4%
total10,109,4141,021,050,814100.0%101.0 bases101 bases92.0%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958246.44.7Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962138.03.0Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.36800
NC_0140350.54986

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input15:52:43 20 Mar 201315:57:16 20 Mar 20134 minutes 33 seconds
Read alignment to reference genome15:57:17 20 Mar 201316:51:28 20 Mar 201354 minutes 11 seconds
Preprocessing alignments for candidate junction identification16:51:28 20 Mar 201317:01:07 20 Mar 20139 minutes 39 seconds
Preliminary analysis of coverage distribution17:01:07 20 Mar 201317:12:22 20 Mar 201311 minutes 15 seconds
Identifying junction candidates17:12:22 20 Mar 201317:12:32 20 Mar 201310 seconds
Re-alignment to junction candidates17:12:32 20 Mar 201317:26:51 20 Mar 201314 minutes 19 seconds
Resolving alignments with junction candidates17:26:51 20 Mar 201317:40:16 20 Mar 201313 minutes 25 seconds
Creating BAM files17:40:16 20 Mar 201317:50:00 20 Mar 20139 minutes 44 seconds
Tabulating error counts17:50:00 20 Mar 201317:53:31 20 Mar 20133 minutes 31 seconds
Re-calibrating base error rates17:53:31 20 Mar 201317:53:35 20 Mar 20134 seconds
Examining read alignment evidence17:53:35 20 Mar 201318:30:41 20 Mar 201337 minutes 6 seconds
Polymorphism statistics18:30:41 20 Mar 201318:30:53 20 Mar 201312 seconds
Output18:30:53 20 Mar 201318:47:48 20 Mar 201316 minutes 55 seconds
Total 2 hours 55 minutes 4 seconds