Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,253,265 Δ1 bp 8.0% coding (239/2421 nt) copA2 ← copper‑transporting P‑type ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,253,2650G→. 8.0% 50.1 ‑0.8 ‑0.3226coding (239/2421 nt)copA2copper‑transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  new base (7/11):  ref base (103/104):  total (111/115)
Fisher's exact test strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.34e-01

Only 100 of 315 total aligned reads displayed.

CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTC  >  NC_014034/1253165‑1253365
                                                                                                     |                                                                                                    
   gCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggg                                                                                                   >  2:6055925/1‑101 (MQ=255)
    cccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.ggggg                                                                                                  >  2:2492714/1‑101 (MQ=255)
    cccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.ggggg                                                                                                  >  1:270878/1‑101 (MQ=255)
     ccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggggg                                                                                                 <  2:1191451/101‑1 (MQ=255)
       tGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggggggg                                                                                               >  2:6258694/1‑101 (MQ=255)
       tGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggggggg                                                                                               >  1:1859688/1‑101 (MQ=255)
       tGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggggggg                                                                                               >  1:6580067/1‑101 (MQ=255)
        gcgcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.ggggggggg                                                                                              >  1:4206229/1‑101 (MQ=255)
          gcATAGGTGACGCGGGCGCTGCCCGCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGc                                                                                            <  2:1733913/101‑1 (MQ=255)
              aGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCg                                                                                        >  1:4478213/1‑101 (MQ=255)
              aGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGa                                                                                       <  1:4388718/101‑1 (MQ=255)
                gTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGaa                                                                                      >  1:3482419/1‑101 (MQ=255)
                gTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGCCGaa                                                                                      >  2:8800/1‑101 (MQ=255)
                   aCGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGcc                                                                                   <  1:1777206/101‑1 (MQ=255)
                    cgcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGccc                                                                                  <  1:838812/101‑1 (MQ=255)
                    cgcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCa                                                                                 <  1:5512871/101‑1 (MQ=255)
                    cgcgGGCGCGGCCCGCGGGAAAAAGCGGGCCCGAGAGCCCCCGGGGCGGCCCCGGCGGGGCGGACCCGACCC.GTCCCGC.GGGGGGGGGGCGTCGAAGCCCa                                                                                 <  1:1929709/101‑1 (MQ=255)
                      ggggCGCTGCCCTCGGGCAAAGACAGGACCGAGAGCACCCCGGGCGTCGCCTTGAGCGCGGACACGGCCCTGCCCGCC.GGGGGGGGGGCGTCGAAGCCCAg                                                                                <  1:4027430/100‑1 (MQ=255)
                      cgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAg                                                                                >  2:4224688/1‑101 (MQ=255)
                      cgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAg                                                                                >  1:474947/1‑101 (MQ=255)
                        ggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGAc                                                                              <  1:6677132/101‑1 (MQ=255)
                         gcgcTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACCCGCCCCTGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGACa                                                                             <  1:6766719/101‑1 (MQ=255)
                          ggcTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAg                                                                            <  1:3679742/100‑1 (MQ=255)
                           gcTGGCCTCGGGCAAAAGCAGGACCGAGGGCCCCCCGGGGGTCGCCGGCAGGGCGGCCCCGCCCCTGGCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAgg                                                                           <  2:522086/101‑1 (MQ=255)
                           gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAgg                                                                           >  2:213263/1‑101 (MQ=255)
                           gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAgg                                                                           >  1:5280108/1‑101 (MQ=255)
                            cTGCCCTCGGGCAAAAGCAGGAACGAGAGCACCCCGGGCGTCGCCTGAAGCGCGGACACGACCCTGTCCGNC.GGGGGGGGGGCGTCGAAGCCAAGACAggc                                                                          >  1:2246773/1‑100 (MQ=255)
                              gCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGGGGTCGAGGCCCAGCCAGGtc                                                                         >  2:4559441/1‑101 (MQ=255)
                              gCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGtcc                                                                        >  2:1687175/1‑100 (MQ=255)
                                ccTCGGGCAAAAGCAGGACCGGGAGCCCCCCGGGCGTCGCCGGCAGCGCGGACACGCCCCTGGCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                      <  1:5663422/101‑1 (MQ=255)
                                ccTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                      <  2:4967727/101‑1 (MQ=255)
                                ccTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCGAAGCCCAGACAGGCctcg                                                                      >  1:3371183/1‑101 (MQ=255)
                                 cTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcgg                                                                     >  2:1872775/1‑101 (MQ=255)
                                 cTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcgg                                                                     >  2:3018125/1‑101 (MQ=255)
                                  tCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACCGGTctcggc                                                                    >  1:5248671/1‑101 (MQ=255)
                                  tCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggc                                                                    >  1:4611185/1‑101 (MQ=255)
                                   cGGGCAAAAGCCGGACCGAGAGCGCCCCGGGCGTCGCCTGCGGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcc                                                                   <  1:1102485/101‑1 (MQ=255)
                                   cGGGCAAAAGCAGGACCGAGAGCGCCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcc                                                                   <  2:277712/101‑1 (MQ=255)
                                    gggCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCGGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcct                                                                  <  2:4100294/101‑1 (MQ=255)
                                    gggCAAAACCAGGACCGGGGGCACCCCGGGCGTCGCCTGCAGCGCGGCCACGACCCTGCCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcct                                                                  <  2:3444025/101‑1 (MQ=255)
                                     ggCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcg                                                                <  1:6087333/101‑1 (MQ=255)
                                      gCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcg                                                                >  2:3991581/1‑101 (MQ=255)
                                       cAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcgg                                                               >  1:2801276/1‑101 (MQ=255)
                                        aaaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcggc                                                              >  1:5381821/1‑101 (MQ=255)
                                          aaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCaa                                                            >  1:697700/1‑101 (MQ=255)
                                          aaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGGCGAAGCCCCGACAGGGCTCGGCCTCGGCaac                                                           >  2:6779231/1‑100 (MQ=255)
                                            gCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATc                                                          >  1:6527565/1‑101 (MQ=255)
                                              aGGACCGAGGGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCgg                                                        <  2:352048/101‑1 (MQ=255)
                                                gACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGAt                                                      <  2:3912775/101‑1 (MQ=255)
                                                gACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGAt                                                      >  1:455501/1‑101 (MQ=255)
                                                  ccGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAg                                                    >  2:6827801/1‑101 (MQ=255)
                                                   cgaggcccccccGGGCTCCGCCTGCAGCCCGGACCCGCCCCTGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                                   <  2:340000/93‑1 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcc                                                  >  2:1332546/1‑101 (MQ=255)
                                                     agagCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                                 <  1:2133041/101‑1 (MQ=255)
                                                     agagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                                 >  2:4799508/1‑101 (MQ=255)
                                                      gagCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccg                                                <  1:4830553/101‑1 (MQ=255)
                                                      gagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccg                                                <  2:6992526/101‑1 (MQ=255)
                                                      gagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgc                                               >  1:2508111/1‑101 (MQ=255)
                                                        gccccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgtccgcc                                              <  1:4234604/98‑1 (MQ=255)
                                                        gCACCCCGGGCGTCGCCTGCAGCGCGGACCCGGCCCTTTCCTCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgcc                                              <  1:4114863/101‑1 (MQ=255)
                                                        gCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGGCGAAGCCCAGACAGGTCTCGGCCTCGGCAAACGGATAgcccccc                                              >  2:5469933/1‑101 (MQ=255)
                                                         ccccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                            <  2:5620037/99‑1 (MQ=255)
                                                         cACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                            <  2:7052778/101‑1 (MQ=255)
                                                          cccccGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                            <  2:4733140/100‑1 (MQ=255)
                                                          cccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                            <  1:1374030/100‑1 (MQ=255)
                                                              tgggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGGCGAAGCCCAGACAGGTCTCGGCCCCGGCAATCGGATAgcccccccccccc                                        >  2:5918703/2‑96 (MQ=255)
                                                              cGGGCGTCGCCTGCAGCGCGGACCCGGCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGcc                                        <  2:4612162/101‑1 (MQ=255)
                                                                 gCGTCGCCTGCAGCGCGGACACGACCCTGGCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgc                                     <  1:5991534/101‑1 (MQ=255)
                                                                  cGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcc                                    >  1:931498/1‑100 (MQ=255)
                                                                  cGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcgc                                   >  1:1630792/1‑101 (MQ=255)
                                                                   gTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCCCGCCAgcgc                                   >  2:1245129/1‑101 (MQ=255)
                                                                     cGCCTGCAGCGCGGCCACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCt                                 <  2:5309598/101‑1 (MQ=255)
                                                                        cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCCGCGCCTCgg                              >  2:3875751/1‑101 (MQ=255)
                                                                          gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATATCCCGCCCGCGCCCGCGCCTCGGcc                            >  2:539512/1‑101 (MQ=255)
                                                                          gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                            <  2:4592924/101‑1 (MQ=255)
                                                                          gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                            >  1:3603816/1‑101 (MQ=255)
                                                                          gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCCCGCCCGCGCCTCGGcc                            >  2:2707453/1‑101 (MQ=255)
                                                                           cAGCGCGGACACGCCCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAg                          <  1:159779/101‑1 (MQ=255)
                                                                            aGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCCGCGCCTCGGcccg                          >  1:756767/1‑99 (MQ=255)
                                                                                cgGCCACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGtt                      <  2:3124536/101‑1 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCCGGGttt                     >  2:4267379/1‑101 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt                     >  1:3197421/1‑101 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCCCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCCGGGCCTCGGCCACGGctt                     >  1:7085198/1‑101 (MQ=255)
                                                                                  gACACGACCCTGTCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc                    <  1:6259013/101‑1 (MQ=255)
                                                                                  gACACGACCCTGTCCGCC.GGGGGGGGGGCGGCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCCGATAACCCCCCCGCGCCAACGCCCCCGCCAGGGGTTc                    >  1:4934323/1‑101 (MQ=255)
                                                                                   gcccGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTcc                   <  2:3197305/98‑1 (MQ=255)
                                                                                   acacGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCCCCTCGGCCCGGGTTTcc                   >  2:3944297/1‑101 (MQ=255)
                                                                                     acGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCCCGGCCAGGGGTTccgc                 >  2:3751856/1‑101 (MQ=255)
                                                                                      cggcccTGTCCCCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc                <  1:29872/98‑1 (MQ=255)
                                                                                      cGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc                >  2:6330255/1‑101 (MQ=255)
                                                                                        aCCCTGTCCGCC.GGGGGGGGGGGGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccc              >  2:4427281/1‑100 (MQ=255)
                                                                                        aCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg              >  1:3740498/1‑101 (MQ=255)
                                                                                        aCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg              >  1:881498/1‑101 (MQ=255)
                                                                                            tGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCCCCTCGGCCAGGGTTTCCGCCCCCGcc          >  1:2298613/1‑101 (MQ=255)
                                                                                             gTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCg         >  2:735070/1‑101 (MQ=255)
                                                                                               ccgcc.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCggg       >  2:5687219/1‑101 (MQ=255)
                                                                                               ccgcc.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGGTTCCGCCCGCGCCggg       >  2:1036443/1‑101 (MQ=255)
                                                                                                     ggggggggggCGTCGAATCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTc  <  2:2246678/101‑1 (MQ=255)
                                                                                                     ggggggggggCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTc  >  2:6490711/1‑101 (MQ=255)
                                                                                                     ggggggggggCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTc  <  1:5946747/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTC  >  NC_014034/1253165‑1253365

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG