Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,255,986 Δ1 bp 5.0% intergenic (+156/+86) RCAP_rcc01184 → / ← RCAP_rcc01185 CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,255,9860G→. 5.0% 31.6 ‑0.9 ‑0.0258intergenic (+156/+86)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (8/5):  ref base (117/128):  total (125/133)
Fisher's exact test strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.47e-01

Only 100 of 281 total aligned reads displayed.

GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAA.G.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084
                                                                                             |                                                                                                    
tgccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.gggggggggg                                                                                             <  1:3497247/91‑1 (MQ=255)
 gccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGa                                                                                            <  2:1856897/92‑1 (MQ=255)
  ccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGaa                                                                                           >  1:2207803/9‑101 (MQ=255)
  cccccccgcccTGGGGGGCCCCCCCCGGGAATTTTCCCCCAAGGGG.AAATGAACCCGGGGCCGGGCTTTCCCCTTGGGCCAAAATTCCC.GGGGGGCGGGaa                                                                                           <  2:3513984/93‑1 (MQ=255)
    ccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACt                                                                                         >  2:625351/7‑101 (MQ=255)
    ccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACt                                                                                         <  1:4849002/95‑1 (MQ=255)
     cccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACtt                                                                                        >  2:5414617/6‑101 (MQ=255)
        gtcgcTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATGCC.GGGGGGGGGGAACTTccc                                                                                     >  1:5607257/5‑101 (MQ=255)
        ggcccGGCGGGTCTGCCCCCGGGATTTTTCCGCCAGGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTccc                                                                                     <  2:7132047/99‑1 (MQ=21)
         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCg                                                                                    >  1:7096718/2‑101 (MQ=255)
            cTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGGCCTTCCCgaa.g                                                                                >  2:921575/1‑101 (MQ=255)
              tcGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCgaa.gga                                                                              <  2:1529953/100‑3 (MQ=255)
              gCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCgaa.gga                                                                              <  2:5872812/101‑3 (MQ=255)
               cGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCGaa.g.gaaa                                                                           <  1:2332965/101‑2 (MQ=255)
                 ggACTCCCCCCGGGATTTTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGaa.g.gaaag                                                                          <  2:4982602/101‑3 (MQ=255)
                 ggACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGaa.g.gaaag                                                                          >  2:2059337/1‑99 (MQ=255)
                 ggACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGaa.g.gaaag                                                                          <  1:2750624/101‑3 (MQ=255)
                   aCTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAagag                                                                         >  1:1001456/1‑101 (MQ=255)
                   aCTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAagag                                                                         >  2:3836555/1‑101 (MQ=255)
                    cTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAgg                                                                        >  1:4752476/1‑101 (MQ=255)
                     tCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAggg                                                                       <  1:2464632/101‑1 (MQ=255)
                      ccccccGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAgggg                                                                      <  1:4387658/101‑1 (MQ=255)
                          ccGGGATATTTCCGGCAGGGGG.AAATGAGCCCGGGGCGCGGCTTTCCCCTGGGGCCAAATGTCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCa                                                                  <  2:2566067/101‑1 (MQ=255)
                           cGGGATATTTCCGCGAAGGGG.AAATGAACCCGGGGCGCGGCTTTCGCCTTGGGCCAAATATCCC..GGGTGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAgg                                                                <  1:3695760/101‑1 (MQ=255)
                           cGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAg                                                                 <  1:6473825/101‑1 (MQ=255)
                           cGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAg                                                                 >  1:4649740/1‑101 (MQ=255)
                            gggATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAgg                                                                <  2:1211318/101‑1 (MQ=255)
                              gATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGcc                                                             >  1:593001/1‑101 (MQ=255)
                               atatTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGccc                                                            >  2:2632576/1‑101 (MQ=255)
                                 tattCCCCCAAGGGGAAAATGACCCGGGGCCCCGGCTTCCCCCTGGGCCAAAATACCCG.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGccc                                                            <  1:5578949/99‑1 (MQ=255)
                                 atTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGcccc                                                           >  1:4452225/1‑101 (MQ=255)
                                 atTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGcccc                                                           <  1:160890/101‑1 (MQ=255)
                                 atTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGcccc                                                           <  1:3093937/101‑1 (MQ=255)
                                  tttCCGGCAAGGGG.AAATGGCCCCGGGGCGCCGCTTTCACCTTGGGCCAAATAGCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGccccc                                                          <  1:2474225/101‑1 (MQ=255)
                                  tttCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCACGAAGG.AAAGAGGGGGGCAGGGccccc                                                          <  2:3723431/101‑1 (MQ=255)
                                   ttCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCt                                                         <  1:4261038/101‑1 (MQ=255)
                                    tCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCCCTTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCtt                                                        <  2:3113390/101‑1 (MQ=39)
                                    tCCGCCAAGGGG.AAATAAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCtt                                                        <  1:3237491/101‑1 (MQ=255)
                                     ccaccAGGGGG.AAATGGACCCGGGGCCCGGCTTCCACCTGGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCttt                                                       <  1:5420659/98‑1 (MQ=37)
                                     ccGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCttt                                                       <  2:1754735/101‑1 (MQ=255)
                                     ccGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCttt                                                       <  1:3877579/101‑1 (MQ=255)
                                      cGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTc                                                      <  1:1028020/101‑1 (MQ=255)
                                       gCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTcc                                                     >  2:3238237/1‑101 (MQ=255)
                                        ccAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTccc                                                    >  1:1001045/1‑101 (MQ=255)
                                         cAAGGGG.AAATGACCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCg                                                   <  1:6541668/101‑1 (MQ=255)
                                         cAAGGGG.AAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTccc                                                    <  1:5997798/101‑1 (MQ=37)
                                         cAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCg                                                   >  1:584385/1‑101 (MQ=255)
                                               g.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCggg                                             >  1:848359/1‑101 (MQ=255)
                                                 aaaTGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCgggg                                            >  2:6540415/1‑101 (MQ=255)
                                                 aaaTGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCgggc                                            >  2:5798706/1‑100 (MQ=255)
                                                  aaTGAACCCGGGGCGCTGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGc                                           >  1:2090423/1‑101 (MQ=255)
                                                   tgggcccgggggCGC.GCATTCCCCGTGGGCCAAATACCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCg                                         <  1:283781/93‑1 (MQ=255)
                                                   aTGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGcc                                          <  2:4744846/101‑1 (MQ=255)
                                                   aTGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGcc                                          >  2:4246823/1‑101 (MQ=255)
                                                   aTGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCg                                         <  2:592981/101‑1 (MQ=255)
                                                     gAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCgg                                        >  1:1940914/1‑101 (MQ=255)
                                                     gAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAA.GAAAAGAGGGGGGCGGGCCCCCCTTTCCGGATGGGGGCGgg                                        >  2:861373/1‑101 (MQ=37)
                                                      aaCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGt                                       >  1:694816/1‑101 (MQ=255)
                                                       aCCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTg                                      <  1:1535578/101‑1 (MQ=255)
                                                       aCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTg                                      >  2:2117873/1‑101 (MQ=255)
                                                       aCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGAGCCGGTg                                      <  1:2058325/101‑1 (MQ=255)
                                                        cccGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGc                                     <  1:1548474/101‑1 (MQ=255)
                                                         ccGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGcc                                    <  2:1001421/101‑1 (MQ=255)
                                                         ccGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGcc                                    <  2:2496611/101‑1 (MQ=255)
                                                           ggggCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCcgc                                  >  1:5108814/1‑101 (MQ=255)
                                                              gcgcGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCcgcgcg                               >  1:5375720/1‑101 (MQ=255)
                                                               cgcgGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCgg                              <  1:1318877/101‑1 (MQ=255)
                                                                   gCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGGACTTCCCGAAGG.AAAGAGGGGGGGAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAgg                          >  2:1068238/1‑101 (MQ=39)
                                                                    cTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAggg                         <  1:5078839/101‑1 (MQ=255)
                                                                    cTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGACCTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGGGCGGCGggg                         >  1:2235844/1‑101 (MQ=38)
                                                                    cTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAggg                         <  2:5071169/101‑1 (MQ=255)
                                                                      ttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGTc                       >  1:1695006/1‑101 (MQ=255)
                                                                       tCACCTTGGGCCAAATATCCC.GGGGGGGGGGCACTTCCCGAAGG.AAAGCGGGGGGGAGGGCTCCGTTTCCCGACTGGGGCCGGCCCCGCGCGGCAGGGTCt                      >  2:5432891/1‑101 (MQ=255)
                                                                        cACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCtt                     <  2:3615275/101‑1 (MQ=255)
                                                                        cACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCtt                     <  1:701981/101‑1 (MQ=255)
                                                                         cccTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTg                    <  2:6678646/100‑1 (MQ=255)
                                                                         aCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGCCTTg                    >  2:5305273/1‑101 (MQ=255)
                                                                          ccTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTgg                   <  2:5477394/101‑1 (MQ=255)
                                                                            ttGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGcc                 >  1:5297432/1‑101 (MQ=255)
                                                                            ttGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGCCTTGGcc                 >  1:1129665/1‑101 (MQ=39)
                                                                             tGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGGCTTGGCCg                >  1:2932509/1‑101 (MQ=255)
                                                                             tGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGCCTTGGCCg                >  1:771187/1‑101 (MQ=255)
                                                                              gggCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCgg               <  2:2252152/101‑1 (MQ=255)
                                                                               ggCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCggg              >  1:3037360/1‑100 (MQ=255)
                                                                                gCCAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCTGGGTCTTGGTCGGTc             <  1:6858235/101‑1 (MQ=39)
                                                                                 ccAAATGGCCC.GGGGGGGGAGCTCTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCa            <  2:1129617/101‑1 (MQ=255)
                                                                                 ccAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGGCa            >  1:2523455/1‑101 (MQ=255)
                                                                                 ccAAATATCCC..GGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAg           <  2:2405405/101‑1 (MQ=255)
                                                                                  cAAATATCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGCCTTGGCCGGTCAg           >  2:4204730/1‑101 (MQ=255)
                                                                                     atatCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGGCAgcgg        >  2:6414435/1‑98 (MQ=255)
                                                                                     atatCCC..GGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcgg       <  2:2970352/101‑1 (MQ=255)
                                                                                      tatCCC.GGTGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcgg       >  1:4532811/1‑101 (MQ=255)
                                                                                      tatCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcgg       >  2:6878645/1‑101 (MQ=255)
                                                                                       atCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcggc      >  2:5977914/1‑101 (MQ=255)
                                                                                       atCCC.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGGCTTGGCCGGGCAggcggg      >  1:5059676/1‑100 (MQ=255)
                                                                                       atCCC.GGGGGGGGGG.ACTTTCCGAAGG.AAAAAGGGGGGGAGGGGCCCCTTTCCCCATCGGGGGCGGGGCCCCGGGGCCGGGGCTTGGCCCGGCCCgcggcg     >  2:7095273/1‑101 (MQ=255)
                                                                                         ccc.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgc    >  2:1976784/1‑101 (MQ=255)
                                                                                           c.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgg  >  1:4128692/1‑100 (MQ=255)
                                                                                           c.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgc  >  1:266102/1‑101 (MQ=255)
                                                                                           c.GGGGGGGGGGAACTTCCCGAAGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgc  >  1:5651278/1‑101 (MQ=255)
                                                                                             |                                                                                                    
GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGG.AAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGAA.G.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG