Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,546 Δ1 bp 10.4% intergenic (+358/‑573) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5460G→.10.4% 164.6 ‑0.6 ‑0.9510intergenic (+358/‑573)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (28/25):  ref base (200/216):  total (247/263)
Fisher's exact test strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.15e-01

Only 100 of 536 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGC  >  NC_014034/1395447‑1395646
                                                                                                   |                                                                                                        
  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                                       <  2:1380059/101‑1 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                  <  1:7136954/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                 <  2:219770/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                 <  2:5348981/101‑1 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                >  1:4167159/1‑101 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                <  2:629795/101‑1 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                >  1:3452898/1‑101 (MQ=255)
               aaaCATCGCCACTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                        <  1:6294285/101‑1 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACTGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                      <  2:1813697/101‑1 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                      <  1:2240420/101‑1 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                      >  2:5682041/1‑101 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                      >  1:6098403/1‑101 (MQ=255)
                 aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.Gc                                                                                     >  1:5636576/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                  >  1:2880381/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                  <  2:1891547/101‑1 (MQ=255)
                    tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcg                                                                                 <  1:644849/101‑1 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                           >  1:2404489/1‑101 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                           >  1:6594888/1‑101 (MQ=255)
                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTAGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcc                                                                          >  1:5100944/1‑101 (MQ=255)
                            ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccc                                                                         >  2:3450246/1‑101 (MQ=255)
                                   tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCgg                                                                  >  1:4334673/1‑101 (MQ=255)
                                         ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                            >  2:6798173/1‑101 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCCCGGGGGCGTg                                                           >  1:3290636/1‑101 (MQ=255)
                                           gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:1054673/91‑1 (MQ=0)
                                           agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTgg                                                          >  2:5993045/11‑101 (MQ=0)
                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTTCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  2:4999435/101‑1 (MQ=255)
                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  1:2766275/101‑1 (MQ=255)
                                            ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  2:47150/10‑101 (MQ=2)
                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:4069645/101‑1 (MQ=255)
                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  1:4400659/1‑101 (MQ=255)
                                             cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:1042213/93‑1 (MQ=2)
                                              gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:4640774/94‑1 (MQ=2)
                                               tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  1:305985/95‑1 (MQ=2)
                                               tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  1:3809706/101‑1 (MQ=255)
                                               ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  2:6378271/95‑1 (MQ=2)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  1:2629332/1‑101 (MQ=255)
                                                 gtccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  2:1726633/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  1:534743/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  1:1566753/5‑101 (MQ=2)
                                                 gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  1:6974482/97‑1 (MQ=2)
                                                 gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  2:2930367/5‑101 (MQ=2)
                                                  gcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  1:8616/98‑1 (MQ=2)
                                                  cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:93175/98‑1 (MQ=2)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:7080361/101‑1 (MQ=255)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:1221654/101‑1 (MQ=255)
                                                   gtgtCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  <  2:2703251/101‑1 (MQ=255)
                                                   cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  <  1:1847456/99‑1 (MQ=2)
                                                   acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  <  1:6010375/99‑1 (MQ=2)
                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                 >  1:1622006/2‑101 (MQ=2)
                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  >  1:4541045/2‑101 (MQ=2)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccc                                                >  2:6615877/1‑100 (MQ=255)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                <  2:6965040/101‑3 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                               >  1:5742484/1‑99 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                               <  1:5571122/101‑1 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                               >  1:3992433/1‑101 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                               <  2:2520841/101‑3 (MQ=2)
                                                      tCGCTCCCCCCGCGGCGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                               <  2:5201044/101‑3 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                              >  1:1778433/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                              >  2:5437696/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgg                                             >  2:4299365/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                             >  1:1129728/1‑97 (MQ=2)
                                                        ccTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                             <  2:1544939/100‑5 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                            >  2:1982530/1‑95 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                            <  1:6741643/101‑7 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TTCAGGCGG.CCC.GC.TCGTCGATCCCCCCGCGGGGGCGGGGATCGAAACCACCTTg                                           >  2:4585460/1‑101 (MQ=37)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                           >  1:233886/1‑94 (MQ=2)
                                                           ccccccGCGGGGTCGTCGGCCAAAACATCTCGGTGCCGTCGCTCG.TCCAGTCGG.CCC.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                          <  1:7067804/101‑1 (MQ=25)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                          >  2:3115889/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                          <  2:5519890/101‑9 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                          >  2:1653188/1‑93 (MQ=2)
                                                              cccGCGGGGGCGTGGATCGAAACATGGCTGTGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccgccacgtct                                       <  2:3386594/101‑11 (MQ=0)
                                                              cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                       <  2:5141672/101‑1 (MQ=255)
                                                               ccGCGGGGGCGCGTCGCGAACCATCTCGCTGCCGTCGCTCG.CCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                      <  2:3305169/101‑1 (MQ=25)
                                                                cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                     <  1:1224457/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                   <  2:6098091/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                   >  1:2548852/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                  <  2:3910860/101‑1 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                  <  2:2064902/101‑1 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                  >  2:2405878/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACCACCTTGCGGATTGTc                                  >  1:6179778/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                 >  2:6210752/1‑101 (MQ=255)
                                                                      ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                               >  2:4078252/1‑101 (MQ=255)
                                                                       gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                              <  1:2073807/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                             <  1:6359709/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                             <  1:4767449/101‑1 (MQ=255)
                                                                           ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                          >  2:279060/1‑101 (MQ=255)
                                                                           ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                          <  1:352923/101‑1 (MQ=255)
                                                                              tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                       >  1:5403001/1‑101 (MQ=255)
                                                                                gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                     >  1:92789/1‑101 (MQ=255)
                                                                                    cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                 >  2:4464570/1‑101 (MQ=255)
                                                                                     aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                <  2:767788/101‑1 (MQ=255)
                                                                                      tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg               <  2:2629236/101‑1 (MQ=255)
                                                                                       ctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt              <  2:4817058/101‑1 (MQ=255)
                                                                                        tcGGTGCCGTCGCTCG.TCCAGCNGN.CNT.GN.NCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc             <  1:2965011/101‑1 (MQ=255)
                                                                                         cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg            <  2:5875549/101‑1 (MQ=255)
                                                                                         cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg            >  1:142237/1‑101 (MQ=255)
                                                                                             gCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGGAGCGTCGCTcc        >  2:4351170/1‑101 (MQ=255)
                                                                                              ccGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc       <  1:1739371/101‑1 (MQ=255)
                                                                                                 tCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc    <  1:1935954/101‑1 (MQ=255)
                                                                                                   gCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc  >  1:3696156/1‑101 (MQ=255)
                                                                                                   |                                                                                                        
CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGC  >  NC_014034/1395447‑1395646

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG