Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,876 Δ1 bp 1.3% intergenic (+688/‑243) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8760C→. 1.3% 8.8 0.0 ‑0.3467intergenic (+688/‑243)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (225/235):  total (228/239)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.33e-01

Only 100 of 495 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCC  >  NC_014034/1395776‑1395976
                                                                                                    |                                                                                                         
   ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                        >  2:1335262/1‑101 (MQ=255)
   ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                        >  2:5637335/1‑101 (MQ=255)
    gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                       <  2:714684/101‑1 (MQ=255)
     ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                                      <  2:5320124/101‑1 (MQ=255)
     ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                                      <  1:6752014/101‑1 (MQ=255)
     ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                                      <  1:5088325/101‑1 (MQ=255)
      gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                     <  1:3924827/101‑1 (MQ=255)
        gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                   >  1:1375471/1‑101 (MQ=255)
          ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...Tc                                                                                              <  2:1537139/101‑1 (MQ=255)
           gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCg                                                                                             >  1:1954574/1‑101 (MQ=255)
             tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                                           >  1:1587539/1‑101 (MQ=255)
             tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                                           >  1:351732/1‑101 (MQ=255)
              cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                          >  2:4525853/1‑101 (MQ=255)
              cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                          <  2:265019/101‑1 (MQ=255)
               gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                         >  2:5046733/1‑101 (MQ=255)
                aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                        <  2:1425726/101‑1 (MQ=255)
                aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgagc                                                                                        >  2:939758/1‑101 (MQ=255)
                aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCCAgcgc                                                                                        >  2:5376513/1‑101 (MQ=255)
                 aaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgc.c                                                                                      <  2:602233/101‑1 (MQ=255)
                   ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAATCTGCGCCGCCTATCGCATCCGG...TCGAGcgc.cgc                                                                                    <  1:5002724/101‑1 (MQ=255)
                    cTGCGGCGTGAGTTGCCAGTCATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.cgcg                                                                                   <  1:5624248/101‑1 (MQ=255)
                     tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.Cgcgc                                                                                  >  2:1008266/1‑101 (MQ=255)
                        ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.Gt                                                                              <  2:4048292/101‑1 (MQ=255)
                           gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGc                                                                           >  1:1683815/1‑101 (MQ=255)
                            tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCt                                                                          <  2:1894351/101‑1 (MQ=255)
                                  gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTcccccc                                                                    >  2:5992330/1‑101 (MQ=255)
                                         aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCggggg                                                             >  2:5188918/1‑101 (MQ=255)
                                         aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCggggg                                                             >  1:4436825/1‑101 (MQ=255)
                                            gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCgg                                                          >  1:5548280/1‑100 (MQ=255)
                                            gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                          <  1:2985900/101‑1 (MQ=255)
                                              gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:3782613/91‑1 (MQ=9)
                                              ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCg                                                           >  1:4052645/10‑101 (MQ=2)
                                               gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  2:4661403/1‑101 (MQ=255)
                                               gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:2419733/1‑101 (MQ=255)
                                                ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                         >  1:4580676/8‑101 (MQ=2)
                                                caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  2:2986494/9‑101 (MQ=9)
                                                 cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:6020280/101‑1 (MQ=255)
                                                  gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:4813314/1‑101 (MQ=255)
                                                  gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:802660/1‑101 (MQ=255)
                                                  gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CCCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:3635253/1‑101 (MQ=255)
                                                  acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:2075198/95‑1 (MQ=9)
                                                   cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGCCCTCGCGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  2:3621060/96‑1 (MQ=9)
                                                   ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  2:6999633/97‑1 (MQ=2)
                                                   ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:5924526/5‑101 (MQ=2)
                                                    cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGGGGAta                                                     >  2:1703380/4‑100 (MQ=2)
                                                     tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:3828038/4‑101 (MQ=9)
                                                     tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                                 >  2:3183540/4‑101 (MQ=9)
                                                     ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:1100936/3‑101 (MQ=2)
                                                     ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:5117031/3‑101 (MQ=2)
                                                     gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:343072/1‑101 (MQ=255)
                                                      gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:1155281/100‑1 (MQ=2)
                                                      atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                <  1:3108723/99‑1 (MQ=9)
                                                      atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                <  1:3731231/99‑1 (MQ=9)
                                                       tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                               <  2:3554508/100‑2 (MQ=9)
                                                       tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                               >  1:4688879/2‑100 (MQ=9)
                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                               <  1:5827852/101‑1 (MQ=255)
                                                        gTCGCTCCCCTCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:4146182/101‑1 (MQ=2)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:2340287/101‑1 (MQ=2)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:2772260/101‑1 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                             >  2:2445926/1‑101 (MQ=255)
                                                            cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                          >  2:5910212/1‑101 (MQ=9)
                                                             tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                         >  2:1060699/4‑100 (MQ=9)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                         >  1:4816346/1‑101 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                        >  1:6938221/1‑101 (MQ=255)
                                                               accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                       >  2:5840928/2‑98 (MQ=9)
                                                                ccccGGGGGGGCGGGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                      <  1:4169571/101‑1 (MQ=255)
                                                                ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                      <  1:2073974/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                  >  1:6586675/1‑93 (MQ=2)
                                                                    gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                  >  1:978833/1‑101 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                  >  1:2533596/1‑101 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                 <  1:6222779/101‑10 (MQ=2)
                                                                      gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                <  2:6124289/101‑11 (MQ=2)
                                                                      gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                >  1:1792065/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                <  2:666427/101‑1 (MQ=255)
                                                                         ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                             <  1:344881/101‑1 (MQ=255)
                                                                         ggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                             <  1:5361781/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                            <  1:1002962/101‑1 (MQ=255)
                                                                           cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                           >  2:4003795/1‑101 (MQ=255)
                                                                           cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                           >  2:5605034/1‑101 (MQ=255)
                                                                           cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                           >  1:3852387/1‑101 (MQ=255)
                                                                             tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                         <  2:2616113/101‑1 (MQ=255)
                                                                             tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                         >  1:6465995/1‑101 (MQ=255)
                                                                              ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                        >  1:1436211/1‑101 (MQ=255)
                                                                               gATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                       >  2:1649804/1‑101 (MQ=255)
                                                                                  cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                    <  1:92348/101‑1 (MQ=255)
                                                                                    aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                  <  2:6448291/101‑1 (MQ=255)
                                                                                    aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                  >  2:6382178/1‑101 (MQ=255)
                                                                                      aCTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGAc                >  2:3361285/1‑101 (MQ=255)
                                                                                           gccgccTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc           <  1:3282969/101‑1 (MQ=255)
                                                                                                 tATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc     <  2:1970474/101‑1 (MQ=255)
                                                                                                 tATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc     >  1:3227138/1‑101 (MQ=255)
                                                                                                 tATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc     <  2:6820176/101‑1 (MQ=255)
                                                                                                  aTCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc    >  1:5727693/1‑101 (MQ=255)
                                                                                                   tCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc   <  2:1153208/101‑1 (MQ=255)
                                                                                                   tCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc   <  2:551582/101‑1 (MQ=255)
                                                                                                    cGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGTGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc  <  1:186387/101‑1 (MQ=255)
                                                                                                    cGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc  <  1:4266759/101‑1 (MQ=255)
                                                                                                    cGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc  <  2:6874827/101‑1 (MQ=255)
                                                                                                    cGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc  <  2:275687/101‑1 (MQ=255)
                                                                                                    cGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc  <  2:4385109/101‑1 (MQ=255)
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GCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCC  >  NC_014034/1395776‑1395976

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG