Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,883 Δ1 bp 1.3% intergenic (+695/‑236) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8830G→. 1.3% 7.6 0.0 ‑0.1467intergenic (+695/‑236)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (221/240):  total (224/243)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.98e-01

Only 100 of 489 total aligned reads displayed.

CGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395783‑1395983
                                                                                                      |                                                                                                          
cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                            <  2:5069139/101‑1 (MQ=255)
cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                            >  1:275691/1‑101 (MQ=255)
 gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                                           >  2:5933265/1‑101 (MQ=255)
   ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                                      <  2:1126056/101‑1 (MQ=255)
    gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                                                                     >  1:1954574/1‑101 (MQ=255)
     aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                                    >  1:3409231/1‑101 (MQ=255)
     aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                                    <  1:4091486/101‑1 (MQ=255)
      tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                                   <  2:7127044/101‑1 (MQ=255)
      tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                                   <  1:1461541/101‑1 (MQ=255)
       cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                                  >  2:4525853/1‑101 (MQ=255)
         aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgagc                                                                                                >  2:939758/1‑101 (MQ=255)
         aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCCAgcgc                                                                                                >  2:5376513/1‑101 (MQ=255)
            ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc.gc                                                                                            >  2:1920034/1‑101 (MQ=255)
              tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                                                                         <  1:421279/101‑1 (MQ=255)
                 ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.Gt                                                                                     <  2:4048292/101‑1 (MQ=255)
                  gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...CCGAGCGCC.GCG.CA.Gtg                                                                                    >  2:3603329/1‑100 (MQ=255)
                      gAGTTGCCAGTTATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTc                                                                                <  2:5688779/101‑1 (MQ=255)
                      gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTc                                                                                <  2:1668703/101‑1 (MQ=255)
                          tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                                            >  1:1153256/1‑101 (MQ=255)
                             cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                                         >  2:3449845/1‑101 (MQ=255)
                             cAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                                         >  1:5070080/1‑101 (MQ=255)
                                 gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                                     >  2:2864232/1‑101 (MQ=255)
                                    aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                  <  2:387930/101‑1 (MQ=255)
                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:3782613/91‑1 (MQ=9)
                                       cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:4007647/101‑1 (MQ=255)
                                        gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                              >  1:1726573/1‑101 (MQ=255)
                                         ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:4839583/101‑1 (MQ=255)
                                         caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:5442037/9‑101 (MQ=9)
                                         caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:2379022/93‑1 (MQ=9)
                                          gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:4712033/95‑1 (MQ=2)
                                          cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  2:1627207/1‑101 (MQ=255)
                                          cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  2:2519661/1‑101 (MQ=255)
                                          aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGGGGATc                                                            >  1:5350814/8‑101 (MQ=9)
                                           gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  2:802660/1‑101 (MQ=255)
                                           acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:5847678/95‑1 (MQ=9)
                                           acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:1310426/95‑1 (MQ=9)
                                            tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:5753739/1‑101 (MQ=255)
                                            cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:3514869/6‑101 (MQ=9)
                                            ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  2:5117400/5‑101 (MQ=2)
                                            ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:6999633/97‑1 (MQ=2)
                                              tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCGCCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  1:1899076/4‑101 (MQ=2)
                                              tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:6246849/4‑101 (MQ=9)
                                              ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  1:5686463/3‑101 (MQ=2)
                                              gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        <  2:6543673/101‑1 (MQ=255)
                                              gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        <  2:1394385/101‑1 (MQ=255)
                                               atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:3731231/99‑1 (MQ=9)
                                               atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:5839722/99‑1 (MQ=9)
                                               aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:6453250/101‑1 (MQ=255)
                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:4815135/1‑101 (MQ=2)
                                                 gTCGCTCCCCTCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        <  2:4146182/101‑1 (MQ=2)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                     >  1:3103248/1‑101 (MQ=255)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                    >  1:3979374/1‑101 (MQ=255)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                    >  2:2632018/1‑101 (MQ=255)
                                                  tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCAGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                    <  1:361773/101‑1 (MQ=2)
                                                    gCTCCCCCCGGGGGGGCGGGGATCGAAACGGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                  <  2:1792732/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                  <  1:4658666/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCCGGGATCCACACGAGGc                                                  >  2:3287587/1‑101 (MQ=37)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                 <  2:2026815/101‑1 (MQ=255)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCCCGGGGGCGCGGATCGAAACGAGGCg                                                 >  1:1793627/1‑101 (MQ=255)
                                                      tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                <  1:322104/98‑2 (MQ=9)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                <  1:2533538/101‑2 (MQ=9)
                                                        cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                              <  1:1335313/101‑1 (MQ=255)
                                                        accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                              >  2:1042178/2‑98 (MQ=9)
                                                         ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                             <  1:2073974/101‑1 (MQ=255)
                                                         ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                             <  1:3267562/101‑1 (MQ=255)
                                                          cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                            <  1:1100342/101‑1 (MQ=255)
                                                           ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                           >  1:1623534/1‑101 (MQ=255)
                                                             gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                         >  2:149507/1‑93 (MQ=2)
                                                             gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                         <  1:7043461/101‑9 (MQ=2)
                                                              cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                        >  1:1394390/1‑92 (MQ=2)
                                                               gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                       <  2:6390351/101‑11 (MQ=2)
                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                       <  2:666427/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                       >  1:4733247/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                       >  1:558831/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                       <  1:6053588/101‑1 (MQ=255)
                                                                   gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                   <  1:1002962/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATGGCCTACGTc                                  >  1:6844405/1‑101 (MQ=255)
                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                  >  1:3852387/1‑101 (MQ=255)
                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                  >  2:5605034/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                 >  2:2677663/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                 >  2:3397873/1‑101 (MQ=255)
                                                                           cGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                           <  1:662498/101‑1 (MQ=255)
                                                                             aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                         <  2:2189770/101‑1 (MQ=255)
                                                                             aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                         >  1:4298060/1‑101 (MQ=255)
                                                                                cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                      <  2:5997403/101‑1 (MQ=255)
                                                                                 tGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                     >  2:5217067/1‑101 (MQ=255)
                                                                                 tGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                     <  1:5992635/101‑1 (MQ=255)
                                                                                  gcg.cCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                    >  1:990790/1‑101 (MQ=255)
                                                                                  gcg.cCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                    <  2:3676748/101‑1 (MQ=255)
                                                                                   cg.ccgc.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                   <  1:298472/101‑1 (MQ=255)
                                                                                           cTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc             <  1:2417609/101‑1 (MQ=255)
                                                                                            tATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc            <  1:964728/101‑1 (MQ=255)
                                                                                            tATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc            >  2:6039829/1‑101 (MQ=255)
                                                                                            tATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc            <  2:6820176/101‑1 (MQ=255)
                                                                                             aTCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc           >  2:3106566/1‑101 (MQ=255)
                                                                                              tCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc          <  2:551582/101‑1 (MQ=255)
                                                                                               cGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc         <  2:4385109/101‑1 (MQ=255)
                                                                                               cGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc         <  2:6874827/101‑1 (MQ=255)
                                                                                                gCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg        <  1:6503493/101‑1 (MQ=255)
                                                                                                  aTCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcccg      >  1:445384/1‑101 (MQ=255)
                                                                                                      |                                                                                                          
CGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395783‑1395983

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG