BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,936	Δ1 bp	11.1%	coding (818/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,936	0	G→.	11.1%	189.1 ‑2.6 ‑0.3	543	coding (818/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/26): ref base (213/266): total (247/296)
Fisher's exact test strand distribution p-value = 7.60e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.29e-01

Only 100 of 562 total aligned reads displayed.


GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTC  >  NC_014034/1396836‑1397036


  cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.Gcc                                                                                                               >  1:1130374/1‑101 (MQ=255)


    cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                            <  1:4936181/101‑1 (MQ=255)


    cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                            >  2:573262/1‑101 (MQ=255)


     tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                           <  1:2196078/101‑1 (MQ=255)


     cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                           <  2:898066/100‑1 (MQ=255)


      ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                          >  1:3993334/1‑101 (MQ=255)


       cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                         <  2:2010936/101‑1 (MQ=255)


          ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc                                                                                                     <  2:1792378/101‑1 (MQ=255)


               ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                              <  1:4593213/101‑1 (MQ=255)


               ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                              >  2:2678799/1‑101 (MQ=255)


                gggCGTTGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                             <  2:5095732/101‑1 (MQ=255)


                gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                             <  2:2474962/101‑1 (MQ=255)


                gatcttGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GA.CGCA.Tc                                                                                             <  1:6575702/96‑1 (MQ=255)


                    gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt                                                                                       >  2:6047217/1‑101 (MQ=255)


                     tGGATCTAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                     <  2:5943423/101‑1 (MQ=255)


                     tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                     <  1:1606272/101‑1 (MQ=255)


                       gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                   <  2:6844051/101‑1 (MQ=255)


                       gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                   <  2:1652424/101‑1 (MQ=255)


                       gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                   <  1:261998/101‑1 (MQ=255)


                           gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAANNN.NNCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.Ct                                                                              >  1:4253190/1‑101 (MQ=255)


                           gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.Ct                                                                              >  2:5670544/1‑101 (MQ=255)


                            aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                             >  2:2114988/1‑101 (MQ=255)


                             aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..c                                                                          <  2:146485/101‑1 (MQ=255)


                               ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..Cgg                                                                        >  2:122390/1‑100 (MQ=255)


                                 ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg.g                                                                     >  1:1651645/1‑101 (MQ=255)


                                  gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.Gt                                                                    <  1:1014352/101‑1 (MQ=255)


                                   tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCGGC.CTG..CGTG.GTc                                                                   >  2:402632/1‑101 (MQ=255)


                                         gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccc                                                             >  1:4431330/1‑101 (MQ=255)


                                          aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                            >  1:3089302/1‑101 (MQ=255)


                                           tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                           <  2:2515112/101‑1 (MQ=255)


                                           tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                           <  1:7091316/101‑1 (MQ=255)


                                            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                          >  2:702831/1‑101 (MQ=255)


                                             gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccccc                                                         >  2:1585898/1‑100 (MQ=255)


                                                 gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                     >  2:3400314/1‑101 (MQ=255)


                                                     ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                 >  2:3857666/1‑101 (MQ=255)


                                                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:6745831/11‑101 (MQ=9)


                                                         attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                             <  2:3541586/91‑1 (MQ=0)


                                                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:6433906/93‑1 (MQ=2)


                                                           tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:1279405/9‑101 (MQ=9)


                                                           gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:4768588/94‑1 (MQ=2)


                                                           cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:3165574/101‑1 (MQ=255)


                                                            caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:5108832/94‑1 (MQ=9)


                                                            caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  1:1464505/8‑101 (MQ=9)


                                                             gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  1:3750729/7‑101 (MQ=2)


                                                              cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:2017662/97‑1 (MQ=2)


                                                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:122391/101‑1 (MQ=255)


                                                              acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:182430/96‑1 (MQ=9)


                                                               aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:702851/101‑1 (MQ=255)


                                                                 gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  1:5854134/100‑1 (MQ=2)


                                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     <  1:6963133/99‑2 (MQ=2)


                                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     >  1:6210111/3‑100 (MQ=2)


                                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     >  1:2822749/3‑100 (MQ=2)


                                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     <  2:2987923/99‑2 (MQ=2)


                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:6413968/1‑101 (MQ=2)


                                                                  cGTCGCTCCCCCCGCCGGGCGGTGGATCCAAACA.GCC..GCTCG.AC.CGCA.TCGCACCG.GGTCC.CCG..CGCC.GCCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                     <  2:2319531/101‑2 (MQ=2)


                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                   <  2:4965321/101‑4 (MQ=2)


                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                   <  2:5790450/101‑4 (MQ=2)


                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                   <  1:1934950/101‑4 (MQ=2)


                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                    <  2:6528541/101‑3 (MQ=9)


                                                                   gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                   <  1:2892583/101‑1 (MQ=255)


                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  <  1:222824/101‑1 (MQ=255)


                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  <  1:2389344/101‑1 (MQ=255)


                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  <  2:4592094/101‑1 (MQ=255)


                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                   >  2:2877016/1‑98 (MQ=9)


                                                                    tCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  <  1:6047529/101‑1 (MQ=255)


                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                    <  1:2715877/101‑2 (MQ=2)


                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACTG.GCCCG.GC.CACG.GC...CTG.TGCGC.TGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                 <  2:2837627/101‑6 (MQ=2)


                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                 >  2:1907558/1‑96 (MQ=2)


                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                >  1:3389383/1‑101 (MQ=255)


                                                                      ccTCCCCCCGCGGGGGCGTGGATCGANNNN.NCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                <  1:79156/100‑1 (MQ=255)


                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                               >  1:553744/1‑94 (MQ=2)


                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                               >  2:4673776/1‑94 (MQ=2)


                                                                         ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                <  1:3819063/101‑3 (MQ=2)


                                                                         ccccccGCGGGGGCGTGGATCGAAACATGGCTAGGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                              <  1:4138348/101‑9 (MQ=2)


                                                                         ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                             >  2:4139063/1‑92 (MQ=2)


                                                                         ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                              >  2:312568/1‑93 (MQ=9)


                                                                         ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                              <  1:5270070/101‑9 (MQ=9)


                                                                         ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                              <  1:5759185/101‑9 (MQ=9)


                                                                          cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                            <  1:960121/101‑1 (MQ=255)


                                                                             ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                            <  2:553732/101‑7 (MQ=0)


                                                                             ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                         <  2:2232829/101‑1 (MQ=255)


                                                                                  ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                    <  1:3225231/101‑1 (MQ=255)


                                                                                  ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                    <  2:3656962/101‑1 (MQ=255)


                                                                                  ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGGCGTTgggc                    >  1:6359208/1‑99 (MQ=255)


                                                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGGCGTTGGGCa                   >  1:4840583/1‑101 (MQ=255)


                                                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                   <  2:6670335/101‑1 (MQ=255)


                                                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                   <  1:566935/101‑1 (MQ=255)


                                                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                <  2:1381927/101‑1 (MQ=255)


                                                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                <  2:1305590/101‑1 (MQ=255)


                                                                                       gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg               >  1:3522486/1‑101 (MQ=255)


                                                                                       gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg               >  2:4231118/1‑101 (MQ=255)


                                                                                            tCGAGACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa          <  2:3306763/101‑1 (MQ=255)


                                                                                            gcGAACCC.GCCG.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa          <  2:2156340/100‑1 (MQ=255)


                                                                                              gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg        <  1:5450038/101‑1 (MQ=255)


                                                                                              gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg        <  2:61725/101‑1 (MQ=255)


                                                                                              gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg        >  2:3196111/1‑101 (MQ=255)


                                                                                               aaaCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt       >  2:749848/1‑101 (MQ=255)


                                                                                                 aCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGCCGGTGTcc     >  2:1073774/1‑101 (MQ=255)


                                                                                                  cA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg    <  2:5332438/101‑1 (MQ=255)


                                                                                                  cA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg    >  1:2465466/1‑101 (MQ=255)


GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTC  >  NC_014034/1396836‑1397036

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG