Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,945 Δ1 bp 11.5% coding (827/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9450G→.11.5% 190.5 ‑1.5 ‑0.7529coding (827/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/26):  ref base (202/223):  total (248/281)
Fisher's exact test strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.06e-01

Only 100 of 559 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCC  >  NC_014034/1396845‑1397045
                                                                                                       |                                                                                                             
     gggggCGTGGATCGAAACCGTTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                         <  1:4368596/101‑1 (MQ=255)
       gatcttGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GA.CGCA.Tc                                                                                                      <  1:6575702/96‑1 (MQ=255)
            tGGATCTAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                              <  2:5943423/101‑1 (MQ=255)
            tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                              <  1:1606272/101‑1 (MQ=255)
             ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tc                                                                                             >  2:5003913/1‑101 (MQ=255)
              gctCGAAACCGGTCCAGAGAGAGACCGCGCGGTGCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                            <  1:4746308/99‑1 (MQ=255)
              gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                            <  1:1314197/101‑1 (MQ=255)
               aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCg                                                                                           >  1:6971733/1‑101 (MQ=255)
                  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGc.cc                                                                                       >  2:684217/1‑100 (MQ=255)
                   aaaCCGGTCCAGAGATATACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                      >  2:2892474/1‑101 (MQ=255)
                   aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                      >  2:2114988/1‑101 (MQ=255)
                   aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                      >  1:2401046/1‑101 (MQ=255)
                     aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..Cg                                                                                  <  2:2655842/101‑1 (MQ=255)
                       cGGTACAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg                                                                                >  2:1644415/1‑101 (MQ=255)
                         gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.Gt                                                                             <  1:1014352/101‑1 (MQ=255)
                          tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCGGC.CTG..CGTG.GTc                                                                            >  2:402632/1‑101 (MQ=255)
                             agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCt                                                                         >  1:148271/1‑101 (MQ=255)
                              gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTc                                                                        <  1:6419238/101‑1 (MQ=255)
                               agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                       <  1:1237402/101‑1 (MQ=255)
                               agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                       <  2:541266/101‑1 (MQ=255)
                                gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                      >  1:1145102/1‑101 (MQ=255)
                                 aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                     <  2:1337654/101‑1 (MQ=255)
                                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCGG.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CCG..CGTG.GGCGCTccccc                                                                    >  1:2265640/1‑101 (MQ=38)
                                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                    <  2:2515112/101‑1 (MQ=255)
                                    gACCGCGCGGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                  <  2:5670868/101‑1 (MQ=255)
                                               cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                       <  1:7123539/101‑1 (MQ=255)
                                               cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CCG..CCGG.GCCGCTCCCCCCGCCGGGGCGTgg                                                       >  1:4962085/1‑101 (MQ=37)
                                                tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                       >  2:1404911/10‑101 (MQ=0)
                                                attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  2:2468786/91‑1 (MQ=0)
                                                 ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     >  2:3867606/10‑101 (MQ=2)
                                                 gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                      >  2:5556729/10‑101 (MQ=9)
                                                  tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:1244526/93‑1 (MQ=2)
                                                  gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:3677160/94‑1 (MQ=2)
                                                  gcggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:2210960/94‑1 (MQ=2)
                                                  cctcGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:2742438/101‑1 (MQ=255)
                                                  ccccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:266896/98‑1 (MQ=255)
                                                   ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  2:6295190/94‑1 (MQ=2)
                                                   ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   >  2:3543444/8‑101 (MQ=2)
                                                   ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:4822804/101‑1 (MQ=255)
                                                   ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:1904131/101‑1 (MQ=255)
                                                   caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTGCCCCCGCGGGGGCGTGGATc                                                    <  2:1786359/94‑1 (MQ=9)
                                                   caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:5108832/94‑1 (MQ=9)
                                                     gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  1:6306006/97‑1 (MQ=2)
                                                      ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGG.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  2:6510198/4‑101 (MQ=2)
                                                      aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                <  1:702851/101‑1 (MQ=255)
                                                      aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                <  2:2154608/101‑1 (MQ=255)
                                                       gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  1:1983397/101‑1 (MQ=255)
                                                       ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               >  1:2480413/4‑101 (MQ=2)
                                                       ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               >  1:4034932/4‑101 (MQ=2)
                                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  1:3332509/100‑1 (MQ=2)
                                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                              >  1:6210111/3‑100 (MQ=2)
                                                        ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 <  2:604060/100‑1 (MQ=2)
                                                         cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                             <  1:3125862/98‑1 (MQ=255)
                                                         cGTCGCTCCCCCCGCCGGGCGGTGGATCCAAACA.GCC..GCTCG.AC.CGCA.TCGCACCG.GGTCC.CCG..CGCC.GCCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                              <  2:2319531/101‑2 (MQ=2)
                                                         cGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                             <  1:592315/101‑1 (MQ=255)
                                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                             <  1:2852264/101‑3 (MQ=2)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                           <  2:4592094/101‑1 (MQ=255)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCCCTCCCCCCGCGGGGGCGTGGGTCCCAACaccc                                           >  2:5744920/1‑98 (MQ=37)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                            >  2:1037758/1‑98 (MQ=9)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                             >  2:1992733/1‑100 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                             >  2:2993651/1‑100 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                           >  1:854282/1‑97 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACTG.GCCCG.GC.CACG.GC...CTG.TGCGC.TGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                          <  2:2837627/101‑6 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                         <  1:3894899/101‑1 (MQ=255)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGcc                                        >  2:1852078/1‑100 (MQ=255)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                        >  1:1683084/1‑94 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                        >  1:553744/1‑94 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatcgga                                        >  1:3202270/1‑93 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                                        >  2:4673776/1‑94 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                         >  2:6159428/1‑99 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                      >  1:4502997/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                      >  2:893597/1‑92 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                      >  2:744687/1‑92 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                       <  1:5759185/101‑9 (MQ=9)
                                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                       <  1:5270070/101‑9 (MQ=9)
                                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                       >  2:312568/1‑93 (MQ=9)
                                                                 cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                      <  2:413998/101‑10 (MQ=2)
                                                                  ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                     <  2:5229637/101‑11 (MQ=2)
                                                                  ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                    <  1:2153006/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                     <  2:7025323/101‑7 (MQ=0)
                                                                     cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                    <  1:3556455/101‑8 (MQ=0)
                                                                         ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                             >  1:6092203/1‑101 (MQ=255)
                                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                            <  2:6670335/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                            <  1:566935/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                            <  2:1127027/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                            <  1:340206/101‑1 (MQ=255)
                                                                              gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                        >  1:6802184/1‑101 (MQ=255)
                                                                              gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                        >  2:4231118/1‑101 (MQ=255)
                                                                              gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                        >  1:3754556/1‑101 (MQ=255)
                                                                                ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                      >  1:3051146/1‑101 (MQ=255)
                                                                                   tCGAGACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                   <  2:3306763/101‑1 (MQ=255)
                                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                 >  2:1512950/1‑101 (MQ=255)
                                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                 >  2:3196111/1‑101 (MQ=255)
                                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                 <  1:5450038/101‑1 (MQ=255)
                                                                                      aaaCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCCC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCAGCTCGGCGTTGGTCAGCCCGACGATGt                >  2:5800637/1‑101 (MQ=38)
                                                                                             ccT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCg          >  1:4640683/1‑101 (MQ=255)
                                                                                               t.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt        <  2:4905796/101‑1 (MQ=255)
                                                                                                    cg.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc    >  1:2202004/1‑101 (MQ=255)
                                                                                                     g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc   >  2:3451264/1‑101 (MQ=255)
                                                                                                       gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc  <  1:4200593/101‑1 (MQ=255)
                                                                                                       |                                                                                                             
CCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCC  >  NC_014034/1396845‑1397045

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG