Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,297 Δ1 bp 2.3% coding (1179/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2970G→. 2.3% 11.7 ‑0.8 ‑0.0298coding (1179/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (138/152):  total (140/158)
Fisher's exact test strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 326 total aligned reads displayed.

ACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1397197‑1397397
                                                                                                       |                                                                                                    
aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                                      <  2:5594282/101‑1 (MQ=255)
aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                                      >  1:3672515/1‑101 (MQ=255)
 cGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGa                                                                                                     >  1:831039/1‑101 (MQ=255)
    gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                                  >  1:3815364/1‑101 (MQ=255)
    gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                                  >  2:2544887/1‑101 (MQ=255)
     tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGt                                                                                                 <  1:1597091/101‑1 (MQ=255)
        gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                              >  2:4180887/1‑101 (MQ=255)
         gcccgacgACCCGCACCAGATCCGCCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCt                                                                                             <  2:2265545/98‑1 (MQ=38)
          cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                                            >  1:6345307/1‑101 (MQ=255)
           ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                           <  2:4938727/100‑1 (MQ=255)
           ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                           <  2:2745230/100‑1 (MQ=255)
            cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                          <  1:4838715/101‑1 (MQ=255)
            cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                          <  2:2158629/101‑1 (MQ=255)
            cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                          >  2:2460648/1‑101 (MQ=255)
             gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                                                                                         <  1:6635970/101‑1 (MQ=255)
              acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                        >  1:5421414/1‑101 (MQ=255)
              acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                        <  1:5503543/101‑1 (MQ=255)
              acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                        >  1:1980130/1‑101 (MQ=255)
               cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                       <  2:6909369/101‑1 (MQ=255)
               cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                       >  1:1274117/1‑101 (MQ=255)
               cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                       <  2:5063208/101‑1 (MQ=255)
                gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                                                                                      <  1:5371390/101‑1 (MQ=255)
                    tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                                                  <  1:2355434/101‑1 (MQ=255)
                     gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                 <  1:3483198/101‑1 (MQ=255)
                     gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                 >  2:1792435/1‑101 (MQ=255)
                       acgaGACCCGCTCCCTCTCCCCCACCGCGGGGGCCGGGATCGAAAC.CCCGC.GGCCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                               <  1:5281842/98‑1 (MQ=255)
                        ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                              >  1:361726/1‑101 (MQ=255)
                        ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                              >  2:140128/1‑101 (MQ=255)
                        ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                              <  2:1718635/101‑1 (MQ=255)
                         cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                                             >  2:4128312/1‑98 (MQ=255)
                         cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                             <  1:1700290/101‑1 (MQ=255)
                          ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  1:2986603/91‑1 (MQ=2)
                              ccggggcGTCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTGCCCCCGCGGGAGCGTGGATc                                                                        >  1:6855213/7‑101 (MQ=38)
                               cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                       <  2:3027740/101‑1 (MQ=255)
                               cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                       >  1:4646763/1‑101 (MQ=255)
                               cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                       <  1:4181082/101‑1 (MQ=255)
                                gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                      >  2:5818015/1‑101 (MQ=255)
                                 gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:4288230/98‑1 (MQ=0)
                                 ccacGTCGCTCCCCCCGCGGGGGCGTGAAGCGAAAC.AAGGCGGCGCGGGCCTATGGCTGTCGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:53700/98‑1 (MQ=2)
                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AAAAA.GCCGCCGACAAACGC.GGCAGCCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  2:3743876/99‑1 (MQ=2)
                                    cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                  >  2:3179529/1‑101 (MQ=255)
                                    cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                  <  2:3867474/101‑1 (MQ=255)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                 >  1:140021/1‑101 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                >  2:4353285/1‑101 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                                              >  1:3337490/1‑95 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                              >  1:5873433/1‑101 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                             <  1:3309317/101‑1 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                             <  2:5615342/101‑1 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                             >  2:2865945/1‑101 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCACCCGCGGGAGCGTGGATCGAAACGGCttt                                                             >  1:4508483/1‑101 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                            <  1:6670419/101‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                           <  1:64839/101‑1 (MQ=255)
                                            cccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                          <  2:895175/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                         <  2:2194276/101‑1 (MQ=255)
                                                cgcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                      <  1:2023617/101‑1 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                     <  1:799123/101‑1 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                     <  1:3765959/101‑1 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                     >  2:811360/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                    <  1:999544/101‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAAAGCCTTc                                                    >  2:6092485/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                                   <  1:1723515/101‑1 (MQ=255)
                                                    ggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTCTACAGCCTTcgc                                                  <  1:5054991/101‑1 (MQ=255)
                                                     gggGGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGATACGGCTTTACAGCCTTcgcg                                                 >  1:5614152/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                                 >  1:970434/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                                 >  1:6185640/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTTACAGCCTTcgcg                                                 >  1:6777058/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGACTTCgcgc                                                >  1:7051687/1‑101 (MQ=255)
                                                        cGTGGGTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                              >  2:3518890/1‑101 (MQ=255)
                                                         gTGGATCGAAAC.ACCGC.GCACGCGCGAGCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                             <  2:1823939/101‑1 (MQ=255)
                                                         gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                             >  1:2183826/1‑101 (MQ=255)
                                                          tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                            <  1:3971088/101‑1 (MQ=255)
                                                          tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                            <  2:3649596/101‑1 (MQ=255)
                                                           ggATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                           <  2:4459897/101‑1 (MQ=255)
                                                           ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                           <  2:6589153/101‑1 (MQ=255)
                                                           ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                           >  2:1265689/1‑101 (MQ=255)
                                                           ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                           >  2:5548719/1‑101 (MQ=255)
                                                            gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                          >  2:4557352/1‑101 (MQ=255)
                                                              tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                        >  2:3689343/1‑101 (MQ=255)
                                                              tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                        <  1:3086659/101‑1 (MQ=255)
                                                                    c.acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                  >  1:4727701/1‑101 (MQ=255)
                                                                         gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                              <  1:4428629/101‑1 (MQ=255)
                                                                         gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                              >  2:5688286/1‑101 (MQ=255)
                                                                          c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                             >  1:1212226/1‑101 (MQ=255)
                                                                             cACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                           <  1:4341028/101‑1 (MQ=255)
                                                                              ccgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                          <  1:4865569/100‑1 (MQ=255)
                                                                               cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                         <  2:6329740/101‑1 (MQ=255)
                                                                                 cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                       <  1:2468204/101‑1 (MQ=255)
                                                                                   cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                     <  2:1980058/101‑1 (MQ=255)
                                                                                   cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                     <  1:2618741/101‑1 (MQ=255)
                                                                                    gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc                    <  2:3380833/101‑1 (MQ=255)
                                                                                     aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                   <  1:6246847/101‑1 (MQ=255)
                                                                                        ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                >  2:5888716/1‑101 (MQ=255)
                                                                                             tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc            >  2:1617121/1‑101 (MQ=255)
                                                                                              cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg           >  1:5123119/1‑101 (MQ=255)
                                                                                               tgCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt          >  2:6667296/2‑101 (MQ=255)
                                                                                               aGCCCCGCGAACGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt          <  2:71307/101‑1 (MQ=255)
                                                                                                   ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt      <  1:4082973/101‑1 (MQ=255)
                                                                                                    cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:856176/101‑1 (MQ=255)
                                                                                                    cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:2518207/101‑1 (MQ=255)
                                                                                                     gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  2:4865981/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
ACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1397197‑1397397

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG