Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,298 Δ1 bp 2.3% coding (1180/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2980A→. 2.3% 10.6 ‑0.8 ‑0.1302coding (1180/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (136/158):  total (138/164)
Fisher's exact test strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.36e-01

Only 100 of 326 total aligned reads displayed.

CGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397198‑1397398
                                                                                                       |                                                                                                    
cGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGa                                                                                                      >  1:831039/1‑101 (MQ=255)
  tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                                    >  1:3961075/1‑101 (MQ=255)
  tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                                    <  1:3779291/101‑1 (MQ=255)
    tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGt                                                                                                  <  1:1597091/101‑1 (MQ=255)
     cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                                                 >  1:949375/1‑101 (MQ=255)
       gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                               >  2:4180887/1‑101 (MQ=255)
         cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                                             >  1:6345307/1‑101 (MQ=255)
          ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                            <  2:2745230/100‑1 (MQ=255)
          ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                            <  2:4938727/100‑1 (MQ=255)
          acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                            >  2:53642/1‑101 (MQ=255)
             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                         <  1:5503543/101‑1 (MQ=255)
             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                         >  1:1980130/1‑101 (MQ=255)
             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                         <  2:5508927/101‑1 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        <  2:5063208/101‑1 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        >  1:1274117/1‑101 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        <  2:6909369/101‑1 (MQ=255)
                       ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                               >  2:140128/1‑101 (MQ=255)
                        cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                                              >  2:4128312/1‑98 (MQ=255)
                        cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                              <  1:1700290/101‑1 (MQ=255)
                        cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                              <  1:2548571/101‑1 (MQ=255)
                         aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                             <  2:1447720/101‑1 (MQ=255)
                          gATCCGCGCCGTGGCGCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                            <  1:7026641/101‑1 (MQ=255)
                          gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                            <  1:3012913/101‑1 (MQ=255)
                           aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa                                                                           >  1:413206/1‑101 (MQ=255)
                             ccggggcGTCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTGCCCCCGCGGGAGCGTGGATc                                                                         >  1:6855213/7‑101 (MQ=38)
                             ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                         <  1:468695/101‑1 (MQ=255)
                              cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                        <  2:3027740/101‑1 (MQ=255)
                               gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                       >  2:5818015/1‑101 (MQ=255)
                                gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  2:4288230/98‑1 (MQ=0)
                                cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                                      <  1:3360062/101‑1 (MQ=255)
                                cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                                      <  1:5132623/101‑1 (MQ=255)
                                 gcCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                     <  2:2249202/101‑1 (MQ=255)
                                  gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AAAAA.GCCGCCGACAAACGC.GGCAGCCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  2:3743876/99‑1 (MQ=2)
                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGGGTGGATCGAAAc                                                                    <  1:5088036/101‑1 (MQ=255)
                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                   <  2:3867474/101‑1 (MQ=255)
                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                   >  2:3179529/1‑101 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                  >  1:140021/1‑101 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                 >  2:4353285/1‑101 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                 <  2:1692843/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                               >  1:5873433/1‑101 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                               <  1:6015275/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                               >  1:2664581/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGccat                                                              >  2:1720686/1‑98 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                              >  2:2865945/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                              <  2:5615342/101‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                             <  1:6670419/101‑1 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                            <  1:64839/101‑1 (MQ=255)
                                           cccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                           <  2:895175/101‑1 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                                         <  2:6651711/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgggcc                                                        >  2:2363584/1‑92 (MQ=0)
                                              ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                        <  1:4260198/101‑10 (MQ=0)
                                               cgcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                       <  1:3504753/101‑1 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                       <  1:2023617/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                      >  1:531769/1‑101 (MQ=255)
                                                gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                      <  1:799123/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                      >  2:811360/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                     <  1:999544/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAAAGCCTTc                                                     >  2:6092485/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                                    <  1:1723515/101‑1 (MQ=255)
                                                    gggGGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGATACGGCTTTACAGCCTTcgcg                                                  >  1:5614152/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                                  <  2:5579068/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTTACAGCCTTcgcg                                                  >  1:6777058/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                                 >  1:3318676/1‑101 (MQ=255)
                                                       cGTGGGTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                               >  2:3518890/1‑101 (MQ=255)
                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                              <  1:4896608/101‑1 (MQ=255)
                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                              >  1:2183826/1‑101 (MQ=255)
                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                              <  2:2014620/101‑1 (MQ=255)
                                                         tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                             <  2:3649596/101‑1 (MQ=255)
                                                          ggATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                            <  2:4459897/101‑1 (MQ=255)
                                                          ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                            <  1:662963/101‑1 (MQ=255)
                                                          ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                            >  2:1265689/1‑101 (MQ=255)
                                                           gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                           >  2:4557352/1‑101 (MQ=255)
                                                            aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACATCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                                          >  2:2470023/1‑101 (MQ=255)
                                                             tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                         >  2:3689343/1‑101 (MQ=255)
                                                             tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                         <  2:1804385/101‑1 (MQ=255)
                                                              cGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                        >  1:436599/1‑101 (MQ=255)
                                                               gAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                       <  1:4009363/101‑1 (MQ=255)
                                                                aaaC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAga                                      >  2:2528051/1‑101 (MQ=255)
                                                                      ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                                 <  1:4839735/101‑1 (MQ=255)
                                                                      ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                                 <  1:3757923/101‑1 (MQ=255)
                                                                       cgc.gCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                                <  2:1832656/101‑1 (MQ=255)
                                                                        gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                               >  2:5688286/1‑101 (MQ=255)
                                                                         c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                              >  1:1212226/1‑101 (MQ=255)
                                                                           gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                             >  1:2372810/1‑101 (MQ=255)
                                                                             aCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                           <  2:2664485/101‑1 (MQ=255)
                                                                              cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                          <  2:6329740/101‑1 (MQ=255)
                                                                               gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc                         >  1:4738896/1‑101 (MQ=255)
                                                                                 gcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc                       <  1:3618832/101‑1 (MQ=255)
                                                                                   gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc                     <  2:3380833/101‑1 (MQ=255)
                                                                                   gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                     >  1:3219232/1‑101 (MQ=255)
                                                                                    aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                    >  1:4558727/1‑101 (MQ=255)
                                                                                    aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                    <  1:6246847/101‑1 (MQ=255)
                                                                                       ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                 >  2:5888716/1‑101 (MQ=255)
                                                                                         c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg               >  1:5404914/1‑101 (MQ=255)
                                                                                            tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc             <  2:2183731/101‑1 (MQ=255)
                                                                                              aGCCCCGCGAACGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt           <  2:71307/101‑1 (MQ=255)
                                                                                                 cccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGa        <  1:4442799/101‑1 (MQ=255)
                                                                                                   cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:856176/101‑1 (MQ=255)
                                                                                                    gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  2:4865981/101‑1 (MQ=255)
                                                                                                       aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  >  2:6526505/1‑101 (MQ=255)
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CGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397198‑1397398

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG