Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,499 Δ1 bp 12.3% coding (1381/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4990G→.12.3% 162.7 ‑0.3 ‑0.4414coding (1381/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/22):  ref base (194/168):  total (223/191)
Fisher's exact test strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.71e-01

Only 100 of 460 total aligned reads displayed.

CTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1397399‑1397599
                                                                                                       |                                                                                                      
ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                                        <  2:3672366/101‑1 (MQ=255)
ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                                        <  2:3815203/101‑1 (MQ=255)
ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                                        >  2:5517244/1‑101 (MQ=255)
 tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACAACCgg                                                                                                       >  2:3343179/1‑101 (MQ=255)
 tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCACTGCACCACCgg                                                                                                       >  1:40630/1‑101 (MQ=255)
  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                      >  1:3342470/1‑101 (MQ=255)
  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                      <  2:290777/101‑1 (MQ=255)
  ctTCAATGGAGATGGCAAAACCGGCCGCGACATGTCGCCCCCCCAGCGGGGGCGTGGATCGAAACCCCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                      <  1:6437337/101‑1 (MQ=38)
   ttCAATGGATATGGCAAAACCGTCCGCGGCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                                     >  1:6287035/1‑101 (MQ=255)
   ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                                     <  1:6733054/101‑1 (MQ=255)
    tCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCg                                                                                                    >  2:4029250/1‑101 (MQ=255)
    tCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCg                                                                                                    >  1:13295/1‑101 (MQ=255)
     cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                                                   >  2:1140941/1‑101 (MQ=255)
     cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                                                   >  1:5127065/1‑101 (MQ=255)
      aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                                                  >  2:6436381/1‑101 (MQ=255)
      aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                                                  >  1:2586339/1‑101 (MQ=255)
       aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                                 >  1:361845/1‑101 (MQ=255)
                gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgg                                                                                        <  2:1572025/101‑1 (MQ=255)
                 cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                                                       <  1:3519026/101‑1 (MQ=255)
                  aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgggg                                                                                      <  1:3578413/101‑1 (MQ=255)
                    aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                                                    <  1:10128/101‑1 (MQ=255)
                       cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                                                 <  2:1212178/101‑1 (MQ=255)
                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTACCCCCGCGGGGGCGTgg                                                                                <  1:4225545/101‑1 (MQ=255)
                          ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  2:3921136/101‑1 (MQ=255)
                           gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                               >  1:2674346/9‑101 (MQ=0)
                             taccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             >  1:4740693/7‑101 (MQ=2)
                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  2:6342075/1‑101 (MQ=255)
                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          <  2:1912911/101‑1 (MQ=255)
                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  1:5684429/1‑101 (MQ=255)
                              cccgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  2:4105243/96‑1 (MQ=2)
                              accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  2:6636145/96‑1 (MQ=2)
                               aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         <  1:6092797/101‑1 (MQ=255)
                                cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                                        >  2:5789607/1‑101 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaac                                                                     >  1:3115698/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcc                                                                    >  1:2081139/1‑101 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                                   <  1:5516019/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                                  >  1:1954531/1‑95 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                                  <  1:327976/101‑7 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAaacggtgtc                                                                  >  1:4403783/1‑95 (MQ=2)
                                          cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                                <  2:4558536/101‑9 (MQ=2)
                                          cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                                <  2:3537101/101‑9 (MQ=2)
                                          cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                                <  2:4452985/101‑9 (MQ=2)
                                           ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                             <  2:5498063/101‑1 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                             <  1:1796691/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagg                                                              >  2:7040932/1‑91 (MQ=2)
                                            cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                                              <  1:3942748/101‑11 (MQ=2)
                                            cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                            <  2:2866556/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                            <  2:6248132/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                           <  2:3211196/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                                         <  1:6544310/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                                         <  2:2344792/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                        >  1:876238/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGATCATCGCCCg                                                       <  1:6855968/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                                       <  1:7070242/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                                       <  2:4876855/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                                   <  2:4199224/101‑1 (MQ=255)
                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                                  >  1:1160417/1‑101 (MQ=255)
                                                       gTGGATCGAAACACCG.TCATC.ACCGCGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                                 <  1:1595007/101‑1 (MQ=255)
                                                         ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                               <  2:6042473/101‑1 (MQ=255)
                                                           aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                                             <  2:2081059/101‑1 (MQ=255)
                                                             tgAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                                           <  2:4346323/100‑1 (MQ=255)
                                                              gAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                                          >  2:6779249/1‑101 (MQ=255)
                                                               aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                                         >  2:2676869/1‑101 (MQ=255)
                                                                 acacCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCGCGCCTCAGCGCCgcgcgg                                       >  2:6918781/1‑99 (MQ=255)
                                                                 acacCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.g                                      >  2:5318140/1‑101 (MQ=255)
                                                                     cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGt                                 <  1:729345/101‑1 (MQ=255)
                                                                     cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGt                                 <  1:2470117/101‑1 (MQ=255)
                                                                      g.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTc                                >  1:4684006/1‑101 (MQ=255)
                                                                         catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGc                              >  2:5865787/1‑101 (MQ=255)
                                                                         catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGc                              >  1:1227324/1‑101 (MQ=255)
                                                                         catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGc                              >  1:989294/1‑101 (MQ=255)
                                                                          atc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCt                             >  2:7099022/1‑101 (MQ=255)
                                                                           tc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTc                            >  1:4996330/1‑101 (MQ=255)
                                                                            c.accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                           <  1:2142847/101‑1 (MQ=255)
                                                                            c.accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                           >  1:4920225/1‑101 (MQ=255)
                                                                             ccccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                           <  2:508273/99‑1 (MQ=255)
                                                                               ccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                         >  2:3012516/1‑101 (MQ=255)
                                                                               ccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                         <  1:1491611/101‑1 (MQ=255)
                                                                               ccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                         <  2:4621514/101‑1 (MQ=255)
                                                                                cacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                        <  2:5362008/101‑1 (MQ=255)
                                                                                 acGCAA.CCGCCCTGCCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                       <  1:2264302/101‑1 (MQ=255)
                                                                                    cAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                    >  2:2200203/1‑101 (MQ=255)
                                                                                          gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc               >  1:5577792/1‑101 (MQ=255)
                                                                                          gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc               <  1:1705566/101‑1 (MQ=255)
                                                                                           cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg              <  2:3967098/101‑1 (MQ=255)
                                                                                             cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg            <  1:5828119/101‑1 (MQ=255)
                                                                                             cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg            <  1:3775345/101‑1 (MQ=255)
                                                                                              tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg           <  1:5375269/101‑1 (MQ=255)
                                                                                              tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg           <  2:1454295/101‑1 (MQ=255)
                                                                                               gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa          >  1:6228860/1‑101 (MQ=255)
                                                                                                  gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GATCCGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  1:4851079/96‑1 (MQ=0)
                                                                                                    gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GATCCGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  1:6913257/4‑101 (MQ=2)
                                                                                                    accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  1:521257/1‑101 (MQ=255)
                                                                                                    accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  2:2485547/1‑101 (MQ=255)
                                                                                                    accGGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACAACCCGATCATGGCCCGCCTCAGCGCAGCGCT.GA.CGTCGCTCCCCCCGCGGGCGAGTGGATCGaa     >  2:4743511/1‑101 (MQ=37)
                                                                                                     ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  2:1777470/101‑1 (MQ=255)
                                                                                                      tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GATCCGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  1:2881944/2‑101 (MQ=2)
                                                                                                      cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  2:6187143/1‑101 (MQ=255)
                                                                                                      cGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  2:4857397/101‑1 (MQ=255)
                                                                                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa  >  2:87395/1‑101 (MQ=255)
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CTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA  >  NC_014034/1397399‑1397599

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG