Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,551 Δ1 bp 9.2% coding (1433/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5510T→. 9.2% 133.0 ‑1.8 ‑0.4524coding (1433/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/28):  ref base (217/201):  total (263/261)
Fisher's exact test strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.95e-01

Only 100 of 549 total aligned reads displayed.

GGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGG  >  NC_014034/1397451‑1397651
                                                                                                       |                                                                                                      
ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                        <  2:4027888/101‑1 (MQ=255)
ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                        <  2:4646524/101‑1 (MQ=255)
 gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                       >  2:2194936/1‑101 (MQ=255)
  cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                                      >  1:1160417/1‑101 (MQ=255)
   gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                     >  1:2449660/1‑101 (MQ=255)
      gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcgc                                                                                                  >  2:6249996/1‑101 (MQ=255)
       aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                                 <  1:6639031/101‑1 (MQ=255)
         tgAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccgc                                                                                               <  2:4346323/100‑1 (MQ=255)
            aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                            >  1:4920649/1‑101 (MQ=255)
             acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.g                                                                                          >  2:5318140/1‑101 (MQ=255)
              cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGGTCATGGC.C..CGCCTCCGCGCCGCGCT.Ga                                                                                         >  2:2416160/1‑101 (MQ=255)
              cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                         >  1:6335568/1‑101 (MQ=255)
                  gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                    >  1:4684006/1‑101 (MQ=255)
                   tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCg                                                                                   >  1:7131550/1‑101 (MQ=255)
                    catcaCCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                  >  1:5078247/1‑101 (MQ=255)
                       caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..GGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                               >  2:199076/1‑101 (MQ=255)
                       caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                               <  2:583081/101‑1 (MQ=255)
                       caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                               >  2:5515133/1‑101 (MQ=255)
                         ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                             >  2:3012516/1‑101 (MQ=255)
                         ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                             <  1:1491611/101‑1 (MQ=255)
                            cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                          <  1:2666845/101‑1 (MQ=255)
                             gccgccGCCCGGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                         <  2:4212504/97‑1 (MQ=255)
                             gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                         >  1:3699672/1‑101 (MQ=255)
                              cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                        <  2:6447937/101‑1 (MQ=255)
                               aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                       >  1:3612383/1‑101 (MQ=255)
                                      cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                <  1:5828119/101‑1 (MQ=255)
                                       tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:5375269/101‑1 (MQ=255)
                                       ggaacaggatgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  2:6941236/91‑1 (MQ=2)
                                        gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                              >  1:6228860/1‑101 (MQ=255)
                                        gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                              >  1:2829019/1‑101 (MQ=255)
                                          cttccgatctgCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:4518618/91‑1 (MQ=2)
                                          cccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:1234243/100‑1 (MQ=255)
                                          accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  1:5224458/1‑101 (MQ=255)
                                          accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:6976695/101‑1 (MQ=255)
                                           cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  1:6461535/7‑101 (MQ=2)
                                            cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:6074509/96‑1 (MQ=0)
                                            cgagcgTCGCGCCCCCCGCGGGGGCGTGGATCGAACCACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GG.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:3569228/96‑1 (MQ=2)
                                            agggtgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  1:4112608/96‑1 (MQ=2)
                                             gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:6913257/4‑101 (MQ=2)
                                             ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:2529757/5‑101 (MQ=2)
                                               cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  2:6187143/1‑101 (MQ=255)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                     >  1:5553194/1‑101 (MQ=255)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                    >  1:530287/1‑101 (MQ=2)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                     <  2:3642207/101‑2 (MQ=2)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                     >  1:3236177/1‑100 (MQ=2)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                    >  2:1840611/1‑101 (MQ=255)
                                                  gcGCGCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                     <  2:3266473/100‑2 (MQ=0)
                                                   cccTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                    <  1:1009470/99‑3 (MQ=2)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                    <  1:6764595/101‑3 (MQ=2)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                   <  1:4029426/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                  <  2:1009332/101‑3 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCTCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                  <  2:4782593/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                  >  2:6365235/1‑101 (MQ=255)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                 <  2:5051721/101‑4 (MQ=2)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                 <  2:1496448/101‑4 (MQ=2)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCcgc                                                 >  2:2896739/1‑101 (MQ=255)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                >  1:5925399/1‑97 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                 <  1:2126380/101‑6 (MQ=0)
                                                      cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                <  1:1476743/100‑5 (MQ=2)
                                                       ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                <  2:3767818/101‑7 (MQ=0)
                                                        cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                              <  1:5649147/101‑7 (MQ=2)
                                                         ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                             >  2:6048782/1‑94 (MQ=2)
                                                          cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                            <  2:725100/101‑9 (MQ=0)
                                                              cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                        >  2:3101736/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                       <  1:1848843/101‑1 (MQ=255)
                                                                   gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                   <  2:5670784/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                  >  1:1597288/1‑101 (MQ=255)
                                                                     gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                                 >  2:834570/1‑100 (MQ=255)
                                                                       ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                               <  1:3497989/101‑1 (MQ=255)
                                                                       ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                               >  1:3325341/1‑101 (MQ=255)
                                                                       ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                               >  2:1298682/1‑101 (MQ=255)
                                                                         aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                             <  1:4834343/101‑1 (MQ=255)
                                                                         aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                             <  2:2110527/101‑1 (MQ=255)
                                                                         aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                             <  2:3953684/101‑1 (MQ=255)
                                                                          tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTGCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                            <  1:5166381/101‑1 (MQ=255)
                                                                          tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                            <  1:2195031/101‑1 (MQ=255)
                                                                           cGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                           >  2:7048006/1‑101 (MQ=255)
                                                                             aaaCACCCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                         <  2:4142187/101‑1 (MQ=255)
                                                                             aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                         >  2:4462846/1‑101 (MQ=255)
                                                                              aacaacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                        <  2:4905911/101‑1 (MQ=255)
                                                                                 aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                     <  2:2667640/101‑1 (MQ=255)
                                                                                 aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                     <  1:4326311/101‑1 (MQ=255)
                                                                                   cccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCggc                   >  1:4862854/1‑100 (MQ=255)
                                                                                   cccGACCATGGC.C..CGCCCCCGCCCCCCGCT.GC.CGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCCCCGGATCGGCGTGACCCGCCCCGCCCATCGGt                   <  1:2267905/101‑1 (MQ=25)
                                                                                     ccgtctgcgC.C..CGCCCCCCCGCCGCCCT.GA.CCTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                 <  2:2274059/93‑1 (MQ=25)
                                                                                       atgacggC.C..CCCCTCAGCGCCGCGCT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt               <  2:5913933/96‑1 (MQ=37)
                                                                                        tCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGCTc              >  1:920977/1‑101 (MQ=255)
                                                                                        tCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc              >  1:3429573/1‑101 (MQ=255)
                                                                                         cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc             >  2:6629065/1‑101 (MQ=255)
                                                                                         cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc             >  1:1685946/1‑101 (MQ=255)
                                                                                          agggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc            <  2:1768122/99‑1 (MQ=255)
                                                                                          aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc            >  1:3838435/1‑101 (MQ=255)
                                                                                          aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc            >  1:1979446/1‑101 (MQ=255)
                                                                                             gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc         >  1:80670/1‑101 (MQ=255)
                                                                                             gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc         <  2:1197048/101‑1 (MQ=255)
                                                                                             gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc         <  2:6825148/101‑1 (MQ=255)
                                                                                              c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg        >  1:3007392/1‑101 (MQ=255)
                                                                                              c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg        >  2:1647479/1‑101 (MQ=255)
                                                                                                   cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg      >  2:2837102/1‑101 (MQ=255)
                                                                                                     ccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg    >  2:3495163/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
GGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGG  >  NC_014034/1397451‑1397651

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG