Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,698 Δ1 bp 1.2% intergenic (+2/+1896) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6980A→. 1.2% 2.4 ‑2.0 ‑0.0338intergenic (+2/+1896)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (145/189):  total (145/193)
Fisher's exact test strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 354 total aligned reads displayed.

CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798
                                                                                                      |                                                                                                     
cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                                                       <  1:3576427/101‑1 (MQ=255)
   ccGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGt                                                                                                    <  1:3206883/101‑1 (MQ=255)
   ccGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGt                                                                                                    <  2:1353467/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTc                                                                                                   <  1:7093897/101‑1 (MQ=255)
        gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.c                                                                                              <  1:2869691/101‑1 (MQ=255)
         accGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.cc                                                                                             <  1:6022474/99‑1 (MQ=255)
          tCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccc                                                                                            >  2:1056816/1‑101 (MQ=255)
          cccgCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccc                                                                                            <  2:1756956/98‑1 (MQ=255)
           cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.cccc                                                                                           >  2:1310082/1‑101 (MQ=255)
            ggCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccccc                                                                                          <  1:874756/101‑1 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccccc                                                                                          >  2:4821437/1‑101 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccccc                                                                                          >  2:1115613/1‑101 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.ccccc                                                                                          >  2:4268260/1‑101 (MQ=255)
             gCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.cccccc                                                                                         <  2:5276835/101‑1 (MQ=255)
             gCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.cccccc                                                                                         <  1:187874/101‑1 (MQ=255)
              cgggACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCcg                                                                                        <  2:3188424/98‑1 (MQ=255)
                tGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCcgcg                                                                                      >  1:4436590/1‑101 (MQ=255)
                   tgaaacccgtccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCgggg                                                                                   >  2:874700/6‑100 (MQ=255)
                   cccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCgggg                                                                                   <  2:2216260/101‑2 (MQ=255)
                     cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGgggc                                                                                 <  1:378533/101‑1 (MQ=255)
                      gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGggcg                                                                                >  1:850225/1‑101 (MQ=255)
                        cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTg                                                                              <  2:3429449/101‑1 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:6559601/1‑101 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:5900632/1‑101 (MQ=255)
                                tCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                      <  1:5342146/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  1:6785540/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACt                                                                    <  1:1298730/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGAGC.CGGGCTTGATCTGTTC.CGGTAACAGGTCGCT.CCCCCCTCGGGGGCGTGGGTCGAAGCt                                                                    >  2:6249014/1‑101 (MQ=25)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTg                                                                  <  1:2397348/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                <  2:1226848/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                >  1:4557253/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                <  2:4422404/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                             >  1:2644916/1‑101 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                             <  1:418918/101‑1 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                                           >  1:5493098/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                                           >  1:1538653/1‑101 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgcg                                                         >  1:1258545/1‑101 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgcg                                                         <  1:299066/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggcg                                                         <  2:661998/101‑7 (MQ=0)
                                                cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                      <  2:4919981/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  2:2881933/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  1:289727/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  2:5216823/1‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                                                    >  2:732954/1‑101 (MQ=255)
                                                   ggtcGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  2:4665803/98‑1 (MQ=255)
                                                   gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  1:2485641/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  1:4223578/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatca                                                  <  2:5041500/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatca                                                  <  2:4710767/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                                                 >  2:5854542/1‑101 (MQ=255)
                                                      cGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                                <  1:6207648/101‑1 (MQ=255)
                                                       gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                               <  1:5846033/101‑1 (MQ=255)
                                                          gATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGt                                            >  1:2146824/1‑101 (MQ=255)
                                                            tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                          <  1:3081060/101‑1 (MQ=255)
                                                            tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                          <  2:4573672/101‑1 (MQ=255)
                                                             cGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCaa                                         >  1:1695314/1‑101 (MQ=255)
                                                             cGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCaa                                         <  2:6749551/101‑1 (MQ=255)
                                                              gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  2:4047924/101‑1 (MQ=255)
                                                               aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       <  2:1716428/101‑1 (MQ=255)
                                                                aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGCTAAGAt                                      >  2:5177595/1‑101 (MQ=255)
                                                                  cTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc                                    <  2:5327353/101‑1 (MQ=255)
                                                                   tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa                                   >  1:405932/1‑101 (MQ=255)
                                                                    aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAg                                  >  1:797737/1‑101 (MQ=255)
                                                                     cAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                 >  1:7033687/1‑101 (MQ=255)
                                                                     cAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                 >  1:6624960/1‑101 (MQ=255)
                                                                      agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCCGCGGGGGGGTGGATCGAAACTTGTCTGCCGCGCCCATCAGCCCGTCACAATTCAgc                                 >  2:6008922/1‑100 (MQ=255)
                                                                       gagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCg                               >  1:6396200/1‑101 (MQ=255)
                                                                          ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                            >  1:496518/1‑101 (MQ=255)
                                                                           tC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc                           <  1:4821680/101‑1 (MQ=255)
                                                                           tC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc                           <  1:2243657/101‑1 (MQ=255)
                                                                              cGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                         >  1:370968/1‑101 (MQ=255)
                                                                               ggTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                        <  2:3782687/101‑1 (MQ=255)
                                                                               ggTCTTGTTCCGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                        <  1:6301064/101‑1 (MQ=255)
                                                                                gTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       >  1:3499659/1‑101 (MQ=255)
                                                                                gTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       <  2:164906/101‑1 (MQ=255)
                                                                                 tCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  2:3632283/101‑1 (MQ=255)
                                                                                  cTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                     <  1:1743842/101‑1 (MQ=255)
                                                                                  cTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                     >  2:3567419/1‑101 (MQ=255)
                                                                                  cTTGTTCTGTTC.AGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCACCACGTCAAGATTCAGTCGCTGCCCCcgg                     >  1:1053707/1‑100 (MQ=37)
                                                                                     gttctgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                  >  2:3098710/1‑101 (MQ=255)
                                                                                      ttctgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgggg                 >  1:1012654/1‑101 (MQ=255)
                                                                                        ctgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  1:6501046/101‑1 (MQ=255)
                                                                                        ctgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:2918876/101‑1 (MQ=255)
                                                                                         tgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg              >  1:1413853/1‑101 (MQ=255)
                                                                                         tgttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg              >  1:6717668/1‑101 (MQ=255)
                                                                                          gttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt             <  2:5528346/101‑1 (MQ=255)
                                                                                           ttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTg            <  1:7001299/101‑1 (MQ=255)
                                                                                           ttc.CGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTg            <  1:5764544/101‑1 (MQ=255)
                                                                                             c.cGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGa          >  1:3329060/1‑101 (MQ=255)
                                                                                               cGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGTGGCGTGGAt         <  2:2043843/101‑1 (MQ=255)
                                                                                               cGGTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:561279/101‑1 (MQ=255)
                                                                                                ggTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:2757031/101‑1 (MQ=255)
                                                                                                ggTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:5217088/101‑1 (MQ=255)
                                                                                                ggTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc        <  2:2644820/101‑1 (MQ=255)
                                                                                                 gTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  1:3937793/101‑1 (MQ=255)
                                                                                                 gTAACAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  2:2568570/101‑1 (MQ=255)
                                                                                                   aaCAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  2:1261468/1‑101 (MQ=255)
                                                                                                    aCAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  2:706715/101‑1 (MQ=255)
                                                                                                     cAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  1:3609527/1‑101 (MQ=255)
                                                                                                      aGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  <  2:6390125/101‑2 (MQ=255)
                                                                                                      |                                                                                                     
CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCT.CCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG