Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,359 Δ1 bp 100% intergenic (+21/+127) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,3590C→.100.0% 487.6 145intergenic (+21/+127)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (18/126):  ref base (1/0):  total (19/126)

Only 100 of 182 total aligned reads displayed.

CAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.G.GGG.GCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAA  >  NC_014034/1843260‑1843452
                                                                                                      |                                                                                                
cAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGc                                                                                                  <  2:2242716/101‑1 (MQ=255)
 aaGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcc                                                                                                 <  1:2140922/101‑1 (MQ=255)
  aGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGccc                                                                                                <  2:819542/101‑1 (MQ=255)
   gCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccc                                                                                               <  2:515801/101‑1 (MQ=255)
   gCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccc                                                                                               <  1:5163461/101‑1 (MQ=255)
     tGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccc                                                                                             <  2:6009473/101‑1 (MQ=255)
      gAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccct                                                                                            <  1:4082806/101‑2 (MQ=255)
       aaaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctc                                                                                           <  1:4753408/101‑3 (MQ=255)
       aaaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctc                                                                                           <  1:897067/101‑3 (MQ=255)
        aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctct                                                                                          <  2:2728760/101‑4 (MQ=255)
        aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctct                                                                                          <  1:1059534/101‑4 (MQ=255)
        aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctct                                                                                          <  2:6565349/101‑4 (MQ=255)
         aaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctctg                                                                                         <  2:3808997/101‑5 (MQ=255)
          aaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctctgc                                                                                        <  1:4377165/101‑6 (MQ=255)
           aGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctctgct                                                                                       <  1:3639685/101‑7 (MQ=255)
             ggCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctctgctgc                                                                                     <  1:344325/101‑9 (MQ=255)
              gcgcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTGcccccctctgctgcg                                                                                    <  2:2512951/101‑10 (MQ=255)
                gcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCa                                                                                 >  2:5126582/1‑101 (MQ=255)
                  gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCAtt                                                                               <  2:7076399/101‑1 (MQ=255)
                   ccccgtgcgAACGGCCTTGTCCAAGACCCCGATTCCATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTc                                                                              <  2:3298159/93‑1 (MQ=255)
                        gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCAcccc                                                                         <  1:5469264/101‑1 (MQ=255)
                          tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCAcccccc                                                                       <  1:765538/101‑1 (MQ=255)
                          tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCACG.GGG.GCGGCCCCCCCCCCCCC.CGCGCTTTCGcccccc                                                                       >  2:6492233/1‑101 (MQ=255)
                           gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                                      <  1:6521692/101‑1 (MQ=255)
                            aaCGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCgg                                                                     <  1:5668953/101‑1 (MQ=255)
                             aCGGGCTTGTCGAAGACCCCGAATACATCGGCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCggg                                                                    <  2:1018011/101‑1 (MQ=37)
                                gCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGata                                                                 <  2:636489/101‑1 (MQ=38)
                                gCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGata                                                                 <  1:2006060/101‑1 (MQ=38)
                                 ccTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGatat                                                                <  1:2861466/101‑1 (MQ=38)
                                 ccTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGatat                                                                <  2:1331318/101‑1 (MQ=38)
                                   ttGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAttt                                                              <  2:2050295/101‑1 (MQ=37)
                                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                             <  2:5290924/101‑1 (MQ=37)
                                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                             <  2:5433353/101‑1 (MQ=37)
                                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                             <  2:5908834/101‑1 (MQ=37)
                                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                             <  2:3968565/101‑1 (MQ=37)
                                     gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTc                                                            <  1:7071450/101‑1 (MQ=37)
                                     gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                            <  1:2477935/101‑1 (MQ=37)
                                     gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                            <  1:6560530/101‑1 (MQ=37)
                                       cGAAGACCCCGATTACATCGTCAAGGCCACGAGCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGc                                                          <  1:3542899/101‑1 (MQ=25)
                                        gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGcc                                                         <  1:5252293/101‑1 (MQ=37)
                                        gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGcc                                                         <  2:2327595/101‑1 (MQ=37)
                                             ccccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGt                                                    <  1:4585916/101‑1 (MQ=37)
                                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                   <  2:4147875/101‑1 (MQ=37)
                                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                   <  1:103594/101‑1 (MQ=37)
                                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                   <  1:1854045/101‑1 (MQ=37)
                                               ccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGa                                                  <  2:571276/101‑1 (MQ=25)
                                               ccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGa                                                  <  2:2326826/101‑1 (MQ=25)
                                                cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                 <  1:2696716/101‑1 (MQ=25)
                                                cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                 <  2:4326248/101‑1 (MQ=25)
                                                cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCA.CCCCCGGGATATTTTCGCCAAGGTGaaa                                                >  1:3016087/1‑101 (MQ=25)
                                                  aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCTCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAt                                               <  1:6349999/101‑1 (MQ=25)
                                                      cATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                           <  2:1442046/101‑1 (MQ=25)
                                                      cATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                           <  1:5452971/101‑1 (MQ=25)
                                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  1:700108/101‑1 (MQ=25)
                                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCTCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  1:5024545/101‑1 (MQ=25)
                                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  2:3081318/101‑1 (MQ=25)
                                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  2:2541877/101‑1 (MQ=25)
                                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCATCTGCTGCGCGTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  1:1120052/101‑1 (MQ=25)
                                                          gTCAAGGCCACGATCGAGTGACACGGGCGCA.GAGGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAgg                                       <  2:5248648/101‑1 (MQ=37)
                                                            cAAGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                                     <  1:583123/101‑1 (MQ=25)
                                                             aaGGCCACGATCGAGTGACACGGGCGCAGG.GGG.GGTCTGCCCCCCCCCCC.TGCGCATTCACCCCCCCCGCCATTTTCTCCCCGGGGAAAAGGAAGAggggg                                    >  2:6779181/1‑99 (MQ=255)
                                                               ggCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                  <  1:6886493/101‑3 (MQ=25)
                                                               ggCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                  <  1:3135314/101‑3 (MQ=25)
                                                                gCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctc                                 <  1:4276352/101‑4 (MQ=25)
                                                                gCCACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctc                                 <  2:6156286/101‑4 (MQ=25)
                                                                 ccACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                <  1:5360866/101‑5 (MQ=25)
                                                                 ccACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                <  1:1643300/101‑5 (MQ=25)
                                                                 ccACGATCGAGTGACACGGGCGCA.G.GGGCGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                <  1:1225813/101‑5 (MQ=25)
                                                                  cACGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                               <  1:6629021/101‑6 (MQ=25)
                                                                    cGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                             <  2:3201131/101‑1 (MQ=25)
                                                                    cGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                             <  2:1476370/101‑1 (MQ=25)
                                                                    cGATCGAGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                             <  1:3029757/101‑1 (MQ=25)
                                                                          aGTGACACGGGCGCAGG.GGG.GCTCTGCCCCCCTCCCC.TGCGCATTCCCCCCCCCGGCAATTTTCGCCAAGGCGAAATGGAAAAGGGCGCTCCGCT.gggggc                      >  2:577299/1‑99 (MQ=255)
                                                                          aGTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                      <  2:5287485/101‑3 (MQ=21)
                                                                           gTGACACGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcg                     <  2:3776637/101‑4 (MQ=21)
                                                                                acGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                 <  1:5991499/101‑1 (MQ=21)
                                                                                acGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                 <  1:2140261/101‑1 (MQ=21)
                                                                                acGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                 <  1:4876964/101‑1 (MQ=21)
                                                                                 cGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCTCCCttt                <  2:322746/101‑1 (MQ=18)
                                                                                 cGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                <  2:6975660/101‑1 (MQ=21)
                                                                                 cGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                <  1:378557/101‑1 (MQ=21)
                                                                                 cGGGCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                <  1:1598118/101‑1 (MQ=21)
                                                                                  gggCGCAGG.GGG.GCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc               <  2:373887/101‑1 (MQ=21)
                                                                                     cgcagg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATc            <  1:1711077/96‑1 (MQ=21)
                                                                                      gcagg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCt           <  1:3491802/97‑1 (MQ=21)
                                                                                       cagg.ggg.gCTCTG.CCCCCCTCTGCTGCGTATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt          <  1:4971157/98‑1 (MQ=21)
                                                                                       cagg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt          <  1:1397215/98‑1 (MQ=21)
                                                                                       cagg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt          <  1:2083366/98‑1 (MQ=21)
                                                                                         gg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc        >  1:3971653/2‑101 (MQ=21)
                                                                                         gg.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc        >  2:4550339/2‑101 (MQ=21)
                                                                                          g.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc       >  2:4886391/1‑101 (MQ=21)
                                                                                          g.ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc       >  2:4384627/1‑101 (MQ=21)
                                                                                            ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca      >  2:6052641/1‑101 (MQ=25)
                                                                                            ggg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca      <  1:6909500/101‑1 (MQ=25)
                                                                                             gg.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac     <  1:5476132/101‑1 (MQ=25)
                                                                                              g.gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcaca    >  2:1690577/1‑101 (MQ=25)
                                                                                                gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa   >  2:3137794/1‑101 (MQ=25)
                                                                                                gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa   >  1:4549645/1‑101 (MQ=25)
                                                                                                 ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  <  1:6981868/101‑1 (MQ=25)
                                                                                                 ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  >  2:6874696/1‑101 (MQ=25)
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CAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.G.GGG.GCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAA  >  NC_014034/1843260‑1843452

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG