Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,355 Δ1 bp 100% intergenic (‑48/+7) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3550C→.100.0% 1588.2 362intergenic (‑48/+7)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (180/181):  ref base (0/0):  total (181/181)

Only 100 of 387 total aligned reads displayed.

TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTGT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGATG  >  NC_014034/1974258‑1974451
                                                                                                    |                                                                                                
tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCAc                                                                                                  >  2:1677624/1‑101 (MQ=255)
 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCAcg                                                                                                 <  1:4327709/101‑2 (MQ=255)
   gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCAcggc                                                                                               <  2:4687749/101‑4 (MQ=255)
    cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCAcggcg                                                                                              <  2:7114477/101‑5 (MQ=255)
     aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGt                                                                                            >  1:4648932/1‑101 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                                                          <  1:2257531/101‑1 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAG.AACGGCGTTc                                                                                          >  2:3054117/1‑101 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                                                          >  1:6915634/1‑101 (MQ=255)
        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                                                         >  2:6497109/1‑101 (MQ=255)
        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                                                         <  1:537250/101‑1 (MQ=255)
         aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAg                                                                                        <  1:4177044/101‑1 (MQ=255)
          ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGt                                                                                       >  2:5376837/1‑101 (MQ=255)
           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                                                      >  1:2115045/1‑101 (MQ=255)
              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                                   >  1:814833/1‑101 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGGTCAGTTCACg                                                                                  >  2:1162672/1‑101 (MQ=255)
                 gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                                                >  1:5972152/1‑101 (MQ=255)
                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                                              >  2:5927232/1‑101 (MQ=255)
                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                                              <  2:4713267/101‑1 (MQ=255)
                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                                             <  1:5037416/101‑1 (MQ=255)
                     tctaTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:5210714/98‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:3246873/101‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:5875152/101‑1 (MQ=255)
                      cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                                           >  2:3871600/1‑101 (MQ=255)
                      cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                                           <  2:860468/101‑1 (MQ=255)
                       gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                                          <  2:3729757/101‑1 (MQ=255)
                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                                         >  1:4393475/1‑101 (MQ=255)
                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                                         >  1:1711181/1‑101 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                       >  1:3352762/1‑101 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                       >  2:1523323/1‑101 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                       <  2:4117667/101‑1 (MQ=255)
                           ttGATGGAACGGTCGACGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                                      <  1:5866272/101‑1 (MQ=255)
                           ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                                      >  1:5745803/1‑101 (MQ=255)
                             gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGCGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                                    <  2:1272555/101‑1 (MQ=255)
                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                                   >  2:5677555/1‑101 (MQ=255)
                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                 <  2:3552811/101‑1 (MQ=255)
                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                 <  1:2674428/101‑1 (MQ=255)
                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                 >  1:632232/1‑101 (MQ=255)
                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                                                <  2:6305874/101‑1 (MQ=255)
                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                                                >  2:4460158/1‑101 (MQ=255)
                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                                               <  2:6136294/101‑1 (MQ=255)
                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                                               >  1:5178980/1‑101 (MQ=255)
                                   aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                                              <  1:1143151/101‑1 (MQ=255)
                                    tccgacggggTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                                             >  2:2168043/9‑101 (MQ=255)
                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                                             >  2:39206/1‑101 (MQ=255)
                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                                             <  1:2720058/101‑1 (MQ=255)
                                     ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                                            <  1:2337942/101‑1 (MQ=255)
                                      gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                                           <  2:5546055/101‑1 (MQ=255)
                                        cGAAGATCATGTCGGACTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                                         >  1:5636679/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGGCCGGATa                                                        >  2:4846723/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                        >  2:4594681/1‑101 (MQ=255)
                                           aGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                                                      >  2:6893289/1‑101 (MQ=255)
                                            ggTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                                     <  1:4734648/101‑1 (MQ=255)
                                            ggTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                                     >  2:4733303/1‑101 (MQ=255)
                                               cATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                                  >  1:1973790/1‑101 (MQ=255)
                                                aTGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                                                 <  2:3667482/101‑1 (MQ=255)
                                                aTGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                                                 <  1:1880544/101‑1 (MQ=255)
                                                 tGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                                                <  1:4524208/101‑1 (MQ=255)
                                                     ggTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc                                            >  2:3353871/1‑101 (MQ=255)
                                                     ggTCTCCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc                                            <  2:4423631/101‑1 (MQ=255)
                                                       tctcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                                          >  2:5043865/1‑101 (MQ=255)
                                                         tcCTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                                        <  2:26451/101‑1 (MQ=255)
                                                          ccTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                                       >  2:1126920/1‑101 (MQ=255)
                                                          ccTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                                       <  1:6219053/101‑1 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                      <  2:5971848/101‑1 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                      >  1:1078241/1‑101 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                      <  1:2434023/101‑1 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                      >  1:1382072/1‑101 (MQ=255)
                                                            tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     <  1:3727828/101‑1 (MQ=255)
                                                            tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     <  2:4862961/101‑1 (MQ=255)
                                                            tGAGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     <  1:394031/101‑1 (MQ=255)
                                                              aGGGGTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                                   >  1:6538486/1‑101 (MQ=255)
                                                               ggggTTGGGGCGGGGGTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                                  <  1:2173629/101‑1 (MQ=255)
                                                               ggggTTGGGGCGGGGGTGTCCCCCCGCCCGGACAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                                  <  1:4858438/101‑1 (MQ=255)
                                                                  gttggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg                               <  1:5510745/92‑1 (MQ=255)
                                                                    tggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                             >  2:3410025/8‑101 (MQ=255)
                                                                     ggggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt                            >  1:4101186/7‑101 (MQ=255)
                                                                       ggcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc                          <  1:3008007/97‑1 (MQ=255)
                                                                        gcgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCg                         <  2:2052334/98‑1 (MQ=255)
                                                                         cgggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa                        <  2:3965744/99‑1 (MQ=255)
                                                                          gggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa                       >  1:1451409/2‑101 (MQ=255)
                                                                          gggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa                       <  2:5126272/100‑1 (MQ=255)
                                                                           ggggTGTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc                      >  1:341796/1‑101 (MQ=255)
                                                                               tgtccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGccc                  >  1:5273675/5‑101 (MQ=255)
                                                                               gaaccccccGCCCGGGTAGCA.CGACGTTCAGTTGACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGccc                  <  1:7054028/97‑1 (MQ=255)
                                                                                gtccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                 >  1:4936342/4‑101 (MQ=255)
                                                                                gtccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                 >  1:1213795/4‑101 (MQ=255)
                                                                                gtccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                 >  2:5815108/4‑101 (MQ=255)
                                                                                gtccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                 >  2:2355330/4‑101 (MQ=255)
                                                                                 tccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCgg                <  1:3564612/99‑1 (MQ=255)
                                                                                  ccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTTGAACGCCCggg               >  1:6687360/2‑101 (MQ=255)
                                                                                  ccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg               <  1:2880453/100‑1 (MQ=255)
                                                                                  ccccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCACAGAGCGACGCGCCAGCCCGGATAGAGATTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg               >  1:6415439/2‑101 (MQ=255)
                                                                                        gcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGtt         <  1:911773/101‑1 (MQ=255)
                                                                                        gcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGtt         <  2:2924897/101‑1 (MQ=255)
                                                                                         cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  1:3054230/101‑1 (MQ=255)
                                                                                         cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  1:1562395/101‑1 (MQ=255)
                                                                                           cgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgc      >  1:3845858/1‑101 (MQ=255)
                                                                                            ggATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGACGGCCCGGGCCAGTTcgcg     >  2:2285964/1‑101 (MQ=255)
                                                                                              aTAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCgcgat   <  1:3162487/101‑1 (MQ=255)
                                                                                               tAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCgcgatg  <  2:4246826/101‑1 (MQ=255)
                                                                                                    |                                                                                                
TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTGT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGATG  >  NC_014034/1974258‑1974451

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG