Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,373 Δ1 bp 96.2% intergenic (‑121/‑267) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3730C→.96.2% 29.3 ‑1.6 ‑0.6334intergenic (‑121/‑267)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (190/131):  ref base (4/7):  total (196/138)
Fisher's exact test strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.76e-01

Only 100 of 362 total aligned reads displayed.

GGCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAA  >  NC_014034/2040272‑2040473
                                                                                                     |                                                                                                     
ggCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAc                                                                                                       <  1:1380244/101‑1 (MQ=255)
ggCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAc                                                                                                       >  1:1614873/1‑101 (MQ=255)
ggCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAc                                                                                                       >  1:4677388/1‑101 (MQ=255)
ggCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAc                                                                                                       >  1:3513677/1‑101 (MQ=255)
 gCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAcc                                                                                                      >  1:1337942/1‑101 (MQ=255)
   aCCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAccgg                                                                                                    <  1:4023203/101‑3 (MQ=255)
     cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                                  >  1:6305007/1‑101 (MQ=255)
      ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                 <  2:4252291/101‑2 (MQ=255)
       gCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCg                                                                                               >  2:6527671/1‑101 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                          <  2:378780/101‑1 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                          <  1:2767039/101‑1 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                          <  2:2203730/101‑1 (MQ=255)
             gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                         <  1:6355125/101‑1 (MQ=255)
             gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                         >  1:3825992/1‑101 (MQ=255)
              ggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCt                                                                                        <  2:6792578/101‑1 (MQ=255)
               gTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCtt                                                                                       >  1:2205926/1‑101 (MQ=255)
                ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                      <  1:6843657/101‑1 (MQ=255)
                ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                      <  1:4422811/101‑1 (MQ=255)
                ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                      >  1:3359683/1‑101 (MQ=255)
                 tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                     <  1:6903458/101‑1 (MQ=255)
                 tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                     >  2:1899425/1‑101 (MQ=255)
                 tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                     >  1:3110124/1‑101 (MQ=255)
                   ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                   >  2:1159297/1‑101 (MQ=255)
                   ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                   <  2:1385016/101‑1 (MQ=255)
                      tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                                >  1:4140829/1‑101 (MQ=255)
                        gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                              >  1:5772639/1‑101 (MQ=255)
                           aaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgc                                                                           >  1:7079744/1‑101 (MQ=255)
                               aCCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGt                                                                       <  1:257009/101‑1 (MQ=255)
                                ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                      <  1:1991674/101‑1 (MQ=255)
                                ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                      <  2:3276307/101‑1 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                    >  1:2363766/1‑101 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                    >  2:4488446/1‑101 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                    <  2:4937243/101‑1 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                    >  2:5013685/1‑101 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                    >  2:5561793/1‑101 (MQ=255)
                                   gAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCa                                                                   >  2:5787570/1‑101 (MQ=255)
                                    aaCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaa                                                                  >  2:6028002/1‑101 (MQ=255)
                                     aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                                 >  1:591205/1‑101 (MQ=255)
                                      cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                >  1:3856141/1‑101 (MQ=255)
                                       ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                               >  1:3001982/1‑101 (MQ=255)
                                       ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                               >  2:6399300/1‑101 (MQ=255)
                                       ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                               >  1:2678794/1‑101 (MQ=255)
                                        gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                              <  2:5356448/101‑1 (MQ=255)
                                        gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                              >  1:2509685/1‑101 (MQ=255)
                                          cAGGGGCCAGACGCAGGCGT.TGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcgg                                                            <  2:5725042/101‑1 (MQ=255)
                                          cAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcgg                                                            >  2:395182/1‑101 (MQ=255)
                                           aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                           >  1:1118072/1‑101 (MQ=255)
                                            ggggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggcg                                                          >  1:6086862/1‑101 (MQ=255)
                                            ggggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCAAAAGAcggag                                                          >  2:1075549/1‑99 (MQ=255)
                                               gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                       <  2:2768538/101‑1 (MQ=255)
                                                ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCg                                                      <  1:6168586/101‑1 (MQ=255)
                                                ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCg                                                      >  2:3541018/1‑101 (MQ=255)
                                                    aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                                  <  2:438087/101‑1 (MQ=255)
                                                      gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                >  2:30396/1‑101 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                             >  2:3613564/1‑100 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                             >  1:5357336/1‑100 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                             >  2:1535322/1‑100 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                             <  2:2634300/101‑2 (MQ=255)
                                                             gtgggggATCCGGT.GAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGcc                                         >  2:6823062/3‑101 (MQ=255)
                                                                  ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                     <  2:7079370/101‑1 (MQ=255)
                                                                  ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                     <  2:1312344/101‑1 (MQ=255)
                                                                  ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                     >  1:5971075/1‑101 (MQ=255)
                                                                  ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                     <  1:6612260/101‑1 (MQ=255)
                                                                     tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTg                                  <  2:889940/101‑1 (MQ=255)
                                                                        ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGc                               >  2:663095/1‑101 (MQ=255)
                                                                         gtggAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              <  1:4488685/98‑1 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              <  2:2860144/101‑1 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              >  1:294994/1‑101 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              <  2:3170143/101‑1 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              >  2:5943395/1‑101 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                              >  1:48808/1‑101 (MQ=255)
                                                                          tCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGccc                             <  1:769228/101‑1 (MQ=255)
                                                                           cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCa                            >  2:4802351/1‑101 (MQ=255)
                                                                           cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCa                            >  2:2512937/1‑101 (MQ=255)
                                                                            gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAc                           >  2:2004884/1‑101 (MQ=255)
                                                                              aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccg                         >  2:784189/1‑100 (MQ=255)
                                                                               ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccgg                        >  2:3379483/1‑99 (MQ=255)
                                                                               ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccgg                        <  2:5645787/101‑3 (MQ=255)
                                                                                  cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                    <  1:5505392/101‑1 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                  <  2:1649211/101‑1 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                  >  1:4301772/1‑101 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                  >  1:2834805/1‑101 (MQ=255)
                                                                                     aCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGa                 <  1:6671053/101‑1 (MQ=255)
                                                                                      cGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAg                <  1:4921071/101‑1 (MQ=255)
                                                                                      cGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAg                >  1:1766763/1‑101 (MQ=255)
                                                                                        ttGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggt              >  2:4645620/1‑100 (MQ=255)
                                                                                         tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt             <  2:5272559/101‑3 (MQ=255)
                                                                                         tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt             >  1:6751993/1‑99 (MQ=255)
                                                                                         tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt             >  2:2957239/1‑99 (MQ=255)
                                                                                           tgCAAAAGCA.CCGGGGCGGATTGCTTACGCGGTATGGCGCGTGCCAAAGACGGTGGCCGCAAAGGCCGCGCCATAAGCGAGGCCA.CCGGTTCGGAggttgg           >  1:5685109/1‑97 (MQ=255)
                                                                                            gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa         <  1:1181122/101‑1 (MQ=255)
                                                                                             cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt        <  2:6197492/101‑1 (MQ=255)
                                                                                             cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt        >  2:4670760/1‑101 (MQ=255)
                                                                                              aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg       <  1:3239067/101‑1 (MQ=255)
                                                                                              aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg       <  2:2313109/101‑1 (MQ=255)
                                                                                              aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg       >  2:4727153/1‑101 (MQ=255)
                                                                                              aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg       >  1:1466578/1‑101 (MQ=255)
                                                                                               aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc      >  2:6603307/1‑101 (MQ=255)
                                                                                                aaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCa     >  2:5093338/1‑101 (MQ=255)
                                                                                                    ccccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa  <  2:539297/100‑1 (MQ=255)
                                                                                                     |                                                                                                     
GGCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAA  >  NC_014034/2040272‑2040473

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG