Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,270 Δ1 bp 100% intergenic (+143/+34) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2700A→.100.0% 23.2 287intergenic (+143/+34)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (173/106):  ref base (1/7):  total (174/113)
Fisher's exact test strand distribution p-value = 6.99e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.84e-01

Only 100 of 338 total aligned reads displayed.

CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAG  >  NC_014034/2044170‑2044366
                                                                                                    |                                                                                                
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCAGCAAAAACCGCGGGGGGAATTTTCACAGCaa                                                                                                 >  1:1541147/1‑101 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                >  2:1015053/1‑101 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                              <  2:2483242/101‑1 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                             <  2:2599137/101‑2 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                             >  2:1734577/1‑100 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGTACAGCaaaaagg                                                                                            <  2:5201454/101‑3 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaagg                                                                                            <  2:829894/101‑3 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaagg                                                                                            >  1:29386/1‑99 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggg                                                                                         <  1:6003474/101‑1 (MQ=255)
           ccccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                       <  1:1173567/99‑2 (MQ=255)
            cccTCCCCCCGAGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcg                                                                                     <  1:4798798/100‑1 (MQ=255)
            aCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcg                                                                                     <  1:5900972/101‑1 (MQ=255)
            aCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcg                                                                                     >  2:5618289/1‑101 (MQ=255)
             ccTCCCCCCGGGCTATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAGGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                    >  1:5097090/1‑101 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                  <  1:1379561/101‑1 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                  <  2:3095487/101‑1 (MQ=255)
                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAg                                                                                <  1:4524007/101‑1 (MQ=255)
                      tggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                           <  2:272180/100‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                           >  2:3926746/1‑101 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                           >  1:6808333/1‑101 (MQ=255)
                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccca                                                                          >  1:272184/1‑100 (MQ=255)
                        gATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCt                                                                         <  2:1212992/101‑1 (MQ=255)
                        gATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCt                                                                         >  1:3010150/1‑101 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                       >  2:5675269/1‑101 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                       <  2:22331/101‑1 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttt                                                                      >  2:3197754/1‑101 (MQ=255)
                            tttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttttt                                                                     >  2:4935965/1‑101 (MQ=255)
                            tttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttttt                                                                     >  1:5774532/1‑101 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttc                                                                    <  1:5407810/101‑2 (MQ=255)
                               gAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttccg                                                                  >  1:3263804/1‑98 (MQ=255)
                                aGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttccgt                                                                 >  2:5893627/1‑97 (MQ=255)
                                 ggCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGGGCGAAGCCCCCC.TTTTTCCGTc                                                               <  2:7026279/101‑1 (MQ=255)
                                  gCAAGATAAAGCCTTGCGAGGTGTTCTTCATCTTTCGGCAAAGCCCGGGGGGGAAATTTGCACAG.AAAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCa                                                              <  2:579702/101‑1 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAg                                                             >  1:3652608/1‑101 (MQ=255)
                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                            >  2:6006040/1‑101 (MQ=255)
                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                            <  2:6267060/101‑1 (MQ=255)
                                     aGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCg                                                           <  2:4190891/101‑1 (MQ=255)
                                      gATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGa                                                          >  1:1576791/1‑101 (MQ=255)
                                      gATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGa                                                          >  2:1120752/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                         >  2:1510285/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                         <  2:6474071/101‑1 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaaa                                                        >  1:1959314/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                       >  2:2849689/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                       >  2:4404017/1‑101 (MQ=255)
                                          aaGTCTTGCGGGGTTTTCTTCTTCTTTCTGCAAAGCCCGGGGGGGAATTTTGCACAG.AAAAAGGGGGGGCGCG.ACCCCCCCCTTTTTCCGTCAGCGAAACa                                                      <  1:2596636/101‑1 (MQ=255)
                                          aaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACa                                                      >  1:6227726/1‑101 (MQ=255)
                                          aaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACa                                                      >  2:4736389/1‑101 (MQ=255)
                                            ggcTTGCGAGGTGTTCTTCATCTTTCTGCAAAGCCCGGGGGGGAAATTTGCACAG.AAAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGc                                                    <  1:1756202/99‑1 (MQ=255)
                                               ttGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAcc                                                 >  1:3767454/1‑101 (MQ=255)
                                               ttGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAcc                                                 >  2:6315162/1‑101 (MQ=255)
                                                 gCGGGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAg                                               <  2:5335534/101‑1 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                             >  2:2994730/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCAAAACAGTACCAgcg                                             >  1:1443339/1‑101 (MQ=255)
                                                     ggTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgcg                                           <  2:4051251/101‑1 (MQ=255)
                                                      gtgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgcgc                                          >  2:6196109/1‑101 (MQ=255)
                                                       ttttcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGcc                                         <  1:5145166/99‑1 (MQ=255)
                                                       tgtTCTTCATCTTTCTGCAACGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAACCAGCACCAGCGCGcc                                         <  1:5618579/101‑1 (MQ=255)
                                                        tttcttcATCTTGCGGAAAGGGCCGGGGGGGGATTTCGCACA.AAAAAAGGGGGGGCGCG.ACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                        <  1:3585799/100‑1 (MQ=255)
                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                        >  1:6349158/1‑101 (MQ=255)
                                                         ttcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                       >  2:1454454/1‑101 (MQ=255)
                                                         ttcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                       >  1:4979707/1‑101 (MQ=255)
                                                         ttcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                       >  1:2282159/1‑101 (MQ=255)
                                                          tcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                      >  2:6597994/1‑101 (MQ=255)
                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                    <  2:5501715/101‑1 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                              >  2:5448264/1‑101 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                              >  1:2175279/1‑101 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                             <  1:5728189/101‑1 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                             <  2:7017137/101‑1 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                             >  1:2854450/1‑101 (MQ=255)
                                                                    tCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                            >  2:3602612/1‑101 (MQ=255)
                                                                      tGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                          >  1:6758028/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                         >  1:2019666/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                         >  1:4105843/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                         >  2:5294467/1‑101 (MQ=255)
                                                                         aaaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                       >  2:3074949/1‑101 (MQ=255)
                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                      >  1:5651837/1‑101 (MQ=255)
                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                      <  1:2214197/101‑1 (MQ=255)
                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                      >  2:6396987/1‑101 (MQ=255)
                                                                           aGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgcg                     >  2:3457205/1‑101 (MQ=255)
                                                                             ccggggggggaAATTTGCACAG.AAAAAAGGGGGCCCCGAGGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                   <  2:4321747/96‑1 (MQ=255)
                                                                             aCCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                   <  1:1734641/101‑1 (MQ=255)
                                                                               cgcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGGCACCCGCGTCTg                 >  2:4567079/1‑101 (MQ=255)
                                                                               cgcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTg                 >  1:1316632/1‑101 (MQ=255)
                                                                               cgcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTg                 >  1:6513826/1‑101 (MQ=255)
                                                                                gcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTgg                >  1:1107418/1‑101 (MQ=255)
                                                                                gcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTgg                >  1:2322065/1‑101 (MQ=255)
                                                                                 cgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTggg               >  1:3889989/1‑101 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGGCGGGCGACCAGGCCACCCGCGTCTgggg              >  1:6183096/1‑100 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt              >  1:696678/1‑101 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt              <  2:5052815/101‑1 (MQ=255)
                                                                                   gggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg             >  2:2350236/1‑101 (MQ=255)
                                                                                   gggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg             >  1:5264747/1‑101 (MQ=255)
                                                                                      ggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg          >  1:1238726/1‑101 (MQ=255)
                                                                                       gAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGa         <  1:772954/101‑1 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt      <  1:5307519/101‑1 (MQ=255)
                                                                                           ttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc     >  1:890291/1‑101 (MQ=255)
                                                                                            tGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc    >  2:6814924/1‑101 (MQ=255)
                                                                                             gCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa   <  2:4521975/101‑1 (MQ=255)
                                                                                             gCACAG.AAAAAGGGGGGGCGCG.ACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa   <  2:2063349/101‑1 (MQ=255)
                                                                                              cacaGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGGGACCAGGCTACCCGCGTCTGGGTGCCGACATCCAg  >  2:797377/1‑101 (MQ=255)
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CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAG  >  NC_014034/2044170‑2044366

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG