Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,403 Δ1 bp 98.2% coding (769/1077 nt) wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4030A→.98.2% 15.1 ‑1.4 ‑1.1341coding (769/1077 nt)wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (139/196):  ref base (4/2):  total (143/198)
Fisher's exact test strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.55e-02

Only 100 of 354 total aligned reads displayed.

CGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGC  >  NC_014034/2123304‑2123498
                                                                                                    |                                                                                                 
cGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGag                                                                                                  >  2:4088449/1‑100 (MQ=255)
 gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGagg                                                                                                 <  2:1108122/101‑3 (MQ=255)
 gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGagg                                                                                                 >  1:6616344/1‑99 (MQ=255)
  aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                >  1:5957176/1‑101 (MQ=255)
   aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                               <  1:5274859/101‑2 (MQ=255)
    gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                             <  2:2195543/101‑1 (MQ=255)
    gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                             >  2:3236687/1‑101 (MQ=255)
     aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                            >  2:578425/1‑101 (MQ=255)
     aTGCCGCGGGCACCGGCCTTGTCCTCGGGCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGAGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                            >  1:5065398/1‑101 (MQ=255)
       gCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGa                                                                                          >  1:193010/1‑101 (MQ=255)
       gCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGa                                                                                          <  2:3095379/101‑1 (MQ=255)
          gcgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cga                                                                                      <  2:3646820/101‑3 (MQ=255)
           cgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.CGGGCTTGA.cgat                                                                                     >  1:7136560/1‑98 (MQ=255)
             ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatct                                                                                   <  2:6122057/101‑6 (MQ=255)
              gCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctg                                                                                  <  1:3837749/101‑7 (MQ=255)
              gCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctg                                                                                  <  1:1130865/101‑7 (MQ=255)
                aTCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggc                                                                                <  2:4881212/101‑9 (MQ=255)
                 tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTCTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggcc                                                                               <  2:3444695/101‑10 (MQ=255)
                  cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggccc                                                                              <  1:5876632/101‑11 (MQ=255)
                            cTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAgg                                                                    <  1:621676/101‑1 (MQ=255)
                            cTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAgg                                                                    >  2:5283141/1‑101 (MQ=255)
                               ggCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcct                                                                 <  2:6466982/101‑1 (MQ=255)
                               ggCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGccc                                                                 >  1:1001050/1‑100 (MQ=255)
                                 ccAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                               <  1:6962916/101‑1 (MQ=255)
                                 ccAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                               <  1:465076/101‑1 (MQ=255)
                                  cAGACCGCCGACGCGGGTGGTC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              <  1:5796381/101‑1 (MQ=255)
                                  cAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              <  2:1018559/101‑1 (MQ=255)
                                  cAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              >  1:5146617/1‑101 (MQ=255)
                                   aGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                             >  1:2562829/1‑101 (MQ=255)
                                   aGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                             >  1:5495347/1‑101 (MQ=255)
                                    gACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGc                                                            <  1:6403119/101‑1 (MQ=255)
                                    gACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGc                                                            <  2:68626/101‑1 (MQ=255)
                                    gACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGc                                                            <  2:764006/101‑1 (MQ=255)
                                      ccgccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                          >  2:2701479/1‑101 (MQ=255)
                                      ccgccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                          <  1:2588853/101‑1 (MQ=255)
                                       cgccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                         <  1:966937/101‑1 (MQ=255)
                                        gccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                        <  2:6989926/101‑1 (MQ=255)
                                        gccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                        >  1:1476236/1‑101 (MQ=255)
                                        gccgACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                        <  2:6229887/101‑1 (MQ=255)
                                            aCGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                    <  1:1352339/101‑1 (MQ=255)
                                             cgcgGGTTATCGGCCGGGTTCGGGGTG.TGGTTTCCCGCGGCCAATCGATTGGCG.AGGGCTTGACC.ATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgc                                                   <  1:248839/101‑1 (MQ=255)
                                                 ggTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcc                                               >  2:3743924/1‑101 (MQ=255)
                                                  gTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                              <  1:5378472/101‑1 (MQ=255)
                                                  gTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                              >  2:832333/1‑101 (MQ=255)
                                                   tGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             >  1:307781/1‑101 (MQ=255)
                                                   tGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             <  2:1195316/101‑1 (MQ=255)
                                                   tGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             >  1:3647574/1‑101 (MQ=255)
                                                    gATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                            >  1:2901363/1‑101 (MQ=255)
                                                    gATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATATGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                            >  2:3006800/1‑101 (MQ=255)
                                                     aTC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                           >  1:3011214/1‑101 (MQ=255)
                                                     aTC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                           >  1:3151849/1‑101 (MQ=255)
                                                         gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                        <  1:3523199/101‑1 (MQ=255)
                                                         gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                        <  2:5105399/101‑1 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      <  1:2385967/101‑1 (MQ=255)
                                                            tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                     <  1:413090/101‑1 (MQ=255)
                                                             gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                    >  2:676229/1‑101 (MQ=255)
                                                             gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                    <  2:4367209/101‑1 (MQ=255)
                                                             gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                    <  2:6295609/101‑1 (MQ=255)
                                                              ggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacga                                   >  2:4176140/1‑101 (MQ=255)
                                                                tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                 <  2:3324205/101‑1 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                >  2:1847800/1‑101 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                >  2:7024033/1‑101 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                <  2:5368218/101‑1 (MQ=255)
                                                                 cAGGGTGATGATTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                <  2:154206/101‑1 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               >  2:2070286/1‑101 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               <  1:1728142/101‑1 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               <  1:4751123/101‑1 (MQ=255)
                                                                   gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                              <  2:5627747/101‑1 (MQ=255)
                                                                    ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                             >  2:2403535/1‑101 (MQ=255)
                                                                    gcgattggtttCCAGCGTCAGATCGATCGGCG.AGGGCTTGC.CGATCTGGCCCAGCAGATAGCCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                             <  2:2302597/93‑1 (MQ=255)
                                                                      tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                           >  1:2085371/1‑101 (MQ=255)
                                                                      tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                           <  1:1826059/101‑1 (MQ=255)
                                                                      tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                           >  1:6326121/1‑101 (MQ=255)
                                                                          gTTTTCTAGCGGCAGAGCGGTCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                       <  2:5880886/101‑1 (MQ=255)
                                                                           ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                      <  1:3246393/101‑1 (MQ=255)
                                                                           ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                      <  2:4812408/101‑1 (MQ=255)
                                                                             ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                    >  2:909910/1‑101 (MQ=255)
                                                                                 aGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                <  2:4985506/101‑1 (MQ=255)
                                                                                 aGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGACGTTTTGCAGCAc                >  2:6395338/1‑101 (MQ=255)
                                                                                  gCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc               <  1:4467149/101‑1 (MQ=255)
                                                                                  gCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc               >  1:6264479/1‑101 (MQ=255)
                                                                                   cGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg              <  1:2665347/101‑1 (MQ=255)
                                                                                   cGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg              <  2:1319094/101‑1 (MQ=255)
                                                                                   cGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg              <  1:4699451/101‑1 (MQ=255)
                                                                                    gTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACCTCGCCGTTTTGCAGCACCgg             >  2:3926545/1‑101 (MQ=255)
                                                                                    gTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg             <  1:3612908/101‑1 (MQ=255)
                                                                                        gatcgatcGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg         >  1:431191/1‑101 (MQ=255)
                                                                                        gatcgatcGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg         >  2:1252341/1‑101 (MQ=255)
                                                                                         atcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt        <  1:831201/101‑1 (MQ=255)
                                                                                          tcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt       <  1:6495903/101‑1 (MQ=255)
                                                                                          tcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt       >  1:3197108/1‑101 (MQ=255)
                                                                                          tcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt       <  1:6809644/101‑1 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      >  2:4404415/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      <  2:5917971/101‑1 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      >  2:1491918/1‑101 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg     >  1:3436408/1‑101 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg     >  1:5234401/1‑101 (MQ=255)
                                                                                             atcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc    <  2:1967058/101‑1 (MQ=255)
                                                                                             atcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc    >  2:1683877/1‑101 (MQ=255)
                                                                                               cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc  >  1:711108/1‑101 (MQ=255)
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CGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATC.GCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGC  >  NC_014034/2123304‑2123498

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG