Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,242 Δ1 bp 100% coding (931/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2420A→.100.0% 1163.7 264coding (931/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (121/143):  ref base (0/0):  total (121/143)

Only 100 of 304 total aligned reads displayed.

GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCC  >  NC_014034/2180143‑2180337
                                                                                                      |                                                                                               
 ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                                                <  1:6794649/101‑1 (MQ=255)
   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                                              >  1:5368430/1‑101 (MQ=255)
      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                                           <  2:4747878/101‑4 (MQ=255)
        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                                        >  1:207942/1‑101 (MQ=255)
         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                                       >  2:7130390/1‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                    <  2:1305509/101‑1 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                    >  1:5787439/1‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                    >  1:5540870/1‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                    >  1:3208160/1‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                    >  1:3137016/1‑101 (MQ=255)
              cttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                                                                                  <  2:4058906/95‑1 (MQ=255)
                 cgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGgcgc                                                                               <  1:5657232/98‑1 (MQ=255)
                  gcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                                                                              >  2:2419310/3‑101 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                           <  2:5343018/95‑1 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                           >  2:3888818/7‑101 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                           <  1:6876219/95‑1 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGACGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                           >  2:6855116/7‑101 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAAGTGCCTCACGTCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGaaa                                                                          >  1:7071775/6‑101 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                                          <  1:4415964/96‑1 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                                          <  2:314562/96‑1 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGAGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                                          >  2:4316677/6‑101 (MQ=255)
                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                                         <  1:3062684/97‑1 (MQ=255)
                        gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                                        <  2:3634203/98‑1 (MQ=255)
                         cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                                       <  1:4660058/99‑1 (MQ=255)
                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                                    <  1:1812550/101‑1 (MQ=255)
                             gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                                   >  1:3327811/1‑101 (MQ=255)
                             gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                                   <  1:2727731/101‑1 (MQ=255)
                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                                  <  1:6490095/101‑1 (MQ=255)
                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                                  >  1:1295639/1‑101 (MQ=255)
                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACCGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGACGCGGCGCCGAAATtcgtcgg                                                                  >  2:4403840/1‑100 (MQ=255)
                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGCAATTCGTCGCAt                                                                >  1:1193906/1‑101 (MQ=255)
                                       ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                                         <  1:1664025/101‑1 (MQ=255)
                                         tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                                       <  2:4945395/95‑1 (MQ=255)
                                         tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                                       <  2:5086263/95‑1 (MQ=255)
                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                                      >  2:2065172/6‑101 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                    <  1:2394073/98‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                   >  2:5990559/3‑101 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                   <  2:3004618/99‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                   <  2:6399058/99‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                   <  2:188712/99‑1 (MQ=255)
                                              ccAAGCATGGCACCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                  >  1:6308390/2‑101 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                  <  2:3303259/100‑1 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                  >  1:1874885/2‑101 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                  <  1:2938633/100‑1 (MQ=255)
                                              ccAAGCATGGCAACCGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                  <  2:2664521/100‑1 (MQ=255)
                                               cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGAGGCGCTGAAATTCGGCGAATGGGCGACCTACAAgg                                                 >  1:1376983/1‑101 (MQ=255)
                                                 aGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGAc                                               >  2:1514550/1‑101 (MQ=255)
                                                  gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                                              <  2:5368158/101‑1 (MQ=255)
                                                   cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                                             >  1:232933/1‑101 (MQ=255)
                                                    aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                                            >  2:162746/1‑101 (MQ=255)
                                                      ggCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTacac                                          >  2:6306891/1‑101 (MQ=255)
                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                                        >  1:6004892/1‑101 (MQ=255)
                                                         aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                                       >  2:2869392/1‑101 (MQ=255)
                                                         aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                                       <  2:6572321/101‑1 (MQ=255)
                                                           cTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGccc                                     <  1:4396225/101‑1 (MQ=255)
                                                           cTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGccc                                     <  2:4650954/101‑1 (MQ=255)
                                                            tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                                    <  2:4550172/101‑1 (MQ=255)
                                                            tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                                    >  2:5743887/1‑101 (MQ=255)
                                                             ggCTCTGGGCCTGGAACCC.CGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                   <  1:4182759/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                   <  1:2465861/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                   <  1:3753344/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCCAATGGGCGACCTCCAAGGACTACACCGCCCtt                                   >  2:5259978/1‑101 (MQ=255)
                                                              gCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTg                                  <  2:5189759/101‑1 (MQ=255)
                                                              gCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTg                                  <  2:5775218/101‑1 (MQ=255)
                                                              gCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTg                                  <  1:2601868/101‑1 (MQ=255)
                                                               ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt                                 <  1:2023837/101‑1 (MQ=255)
                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                               >  1:487213/1‑101 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTAGTAGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                             >  2:5632222/1‑101 (MQ=255)
                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa                            >  2:2577340/1‑101 (MQ=255)
                                                                      ccTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGc                          <  2:5435120/101‑1 (MQ=255)
                                                                      ccTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGc                          <  1:3617941/101‑1 (MQ=255)
                                                                       cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGGCCTCCAAGGGCTACACCGCCCTTGGCGcaggca                         >  1:2503715/1‑96 (MQ=255)
                                                                       cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCa                         <  2:6490487/101‑1 (MQ=255)
                                                                       cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCa                         >  1:5191988/1‑101 (MQ=255)
                                                                          ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag                       >  1:6680602/5‑101 (MQ=255)
                                                                             aacctagccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagga                     >  2:3512229/10‑101 (MQ=255)
                                                                               cTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCacggaagg                  >  1:1024729/1‑101 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                 >  1:5514168/1‑101 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                 >  1:5872847/1‑101 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                 <  2:1676715/101‑1 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                 <  2:400876/101‑1 (MQ=255)
                                                                                  cgccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCt                <  1:5041264/97‑1 (MQ=255)
                                                                                   gccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTg               <  2:5667248/98‑1 (MQ=255)
                                                                                   gccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTg               <  2:6062181/98‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg              <  1:3868928/99‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg              <  1:5320807/99‑1 (MQ=255)
                                                                                     caTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCACCAAGGAAGGCTggg             >  2:1159575/2‑101 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCCGCAAGGAAGGCTGGGc            >  1:448591/1‑101 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc            <  2:4875194/101‑1 (MQ=255)
                                                                                       ttCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCg           >  1:5260641/1‑101 (MQ=255)
                                                                                         ccGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTTATTTTCAACGATTCGGCGACCTCCGAGATCTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGaa         <  2:1376932/101‑1 (MQ=255)
                                                                                         ccGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGaa         <  2:1008731/101‑1 (MQ=255)
                                                                                         ccGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGaa         <  2:6725646/101‑1 (MQ=255)
                                                                                          cggcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAAc        >  1:2829169/1‑101 (MQ=255)
                                                                                            gcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGt      <  1:654181/101‑1 (MQ=255)
                                                                                             cggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACggg     >  2:3492572/1‑99 (MQ=255)
                                                                                             caggCACGC.AAAGGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCTTCCAACAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTg     <  2:2330246/99‑1 (MQ=255)
                                                                                              gggCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACAGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGc    <  1:5461699/101‑1 (MQ=255)
                                                                                                gCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGccc  <  2:555737/101‑1 (MQ=255)
                                                                                                gCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGCAAGGCTGGGCGAACGTGccc  <  1:6383718/101‑1 (MQ=255)
                                                                                                      |                                                                                               
GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCC  >  NC_014034/2180143‑2180337

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG