Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,198,718 Δ1 bp 96.9% coding (151/729 nt) cobA2 ← uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,198,7180C→.96.9% 16.7 ‑0.7 ‑0.9260coding (151/729 nt)cobA2uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (141/111):  ref base (3/5):  total (144/116)
Fisher's exact test strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.35e-01

Only 100 of 276 total aligned reads displayed.

ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816
                                                                                                    |                                                                                                  
   cGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccc                                                                                                 >  2:2601077/1‑101 (MQ=255)
   cGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccc                                                                                                 <  2:5117349/101‑1 (MQ=255)
    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                >  2:4558953/1‑101 (MQ=255)
    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                <  1:2835659/101‑1 (MQ=255)
              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     <  2:4469257/101‑1 (MQ=255)
              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     >  1:2783804/1‑101 (MQ=255)
                gcgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATgg                                                                                   <  2:1076043/101‑1 (MQ=255)
                 cgcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGc                                                                                  <  1:2419503/101‑1 (MQ=255)
                  gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 <  1:556738/101‑1 (MQ=255)
                  gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 >  2:6220739/1‑101 (MQ=255)
                  ccATGATGGGCAAGGAAGCGGCTGACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 <  2:5072686/100‑1 (MQ=255)
                   cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                <  1:1419282/101‑1 (MQ=255)
                    aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                               >  2:1752182/1‑100 (MQ=255)
                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              <  2:5568909/101‑3 (MQ=255)
                     tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                              <  1:3290863/101‑3 (MQ=255)
                      gATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccagg                                                                             <  2:2115073/101‑4 (MQ=255)
                       aTCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGGCGCCCGGCCCGCTTGCCGACCCCGATGAGTTCGG.CCCCCGGGCGGGCGTGGcccagga                                                                            >  1:6288451/1‑97 (MQ=255)
                       aTCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGGCCGGCCGGCTTGCCGACACGGATGAGTTGGGCGCCCC.GGCGGGCATGGCCCCGgg                                                                            >  2:1579271/1‑101 (MQ=255)
                       aTCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCC.GGCGGGCATGGcccagga                                                                            >  2:4760958/1‑97 (MQ=255)
                        tCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGAt                                                                          <  2:4226291/101‑1 (MQ=255)
                         cgacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATc                                                                         >  1:1159731/1‑101 (MQ=255)
                         cgacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCAGGCGGGCATGG.CCCAGGATc                                                                         >  1:4361053/1‑101 (MQ=255)
                          gacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGTCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCA.GGGCCCAGGATCg                                                                        <  1:5099101/101‑1 (MQ=255)
                          gacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCg                                                                        <  2:1306398/101‑1 (MQ=255)
                           acgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCgg                                                                       >  2:5282173/1‑101 (MQ=255)
                           acgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCgg                                                                       >  1:5160051/1‑101 (MQ=255)
                            cgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGc                                                                      <  1:2592631/101‑1 (MQ=255)
                             gaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcc                                                                     >  1:5400659/1‑101 (MQ=255)
                             gaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcc                                                                     <  1:961415/101‑1 (MQ=255)
                              aGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGccc                                                                    >  2:5715222/1‑101 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  <  2:6979755/101‑3 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  1:4718254/1‑99 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  <  1:79599/101‑3 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  1:2687429/1‑99 (MQ=255)
                                  aGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggcg                                                                <  1:1811908/101‑5 (MQ=255)
                                     ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                            <  2:5429550/101‑1 (MQ=255)
                                      gCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaaa                                                           <  1:6884436/101‑1 (MQ=255)
                                      gCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaaa                                                           >  2:1206026/1‑101 (MQ=255)
                                       cTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAg                                                          <  2:1689107/101‑1 (MQ=255)
                                        tCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGa                                                         >  2:838358/1‑101 (MQ=255)
                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                       <  1:5858619/101‑1 (MQ=255)
                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                       <  2:2783702/101‑1 (MQ=255)
                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  2:4958906/1‑101 (MQ=255)
                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  1:198271/1‑101 (MQ=255)
                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  2:881449/1‑101 (MQ=255)
                                             tGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtc                                                    >  2:3822281/1‑101 (MQ=255)
                                                tCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGaa                                                 >  2:200216/1‑101 (MQ=255)
                                                tCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGaa                                                 <  1:4501956/101‑1 (MQ=255)
                                                 ccTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAAc                                                <  1:65801/101‑1 (MQ=255)
                                                   tGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAg                                              <  2:4002543/101‑1 (MQ=255)
                                                   tGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAg                                              <  1:3539754/101‑1 (MQ=255)
                                                      ttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcac                                           >  2:1189081/1‑101 (MQ=255)
                                                       tCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacc                                          <  2:56739/101‑1 (MQ=255)
                                                       tCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacc                                          <  1:1498892/101‑1 (MQ=255)
                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        >  1:7103909/1‑101 (MQ=255)
                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        <  1:1579332/101‑1 (MQ=255)
                                                          tcctgggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACcaca                                       >  2:5162813/5‑101 (MQ=255)
                                                          gCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACcaca                                       >  1:4100270/1‑101 (MQ=255)
                                                          gCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACcaca                                       >  2:2380793/1‑101 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  1:4762175/1‑101 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     <  1:72964/101‑1 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  1:36205/1‑101 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  2:4311338/1‑101 (MQ=255)
                                                              gggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATctg                                   >  2:360094/1‑99 (MQ=255)
                                                              gggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCgg                                   <  1:664224/101‑1 (MQ=255)
                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                              >  2:6340054/1‑101 (MQ=255)
                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                              <  2:1603725/101‑1 (MQ=255)
                                                                    cccggcccgCTTGCGGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTgg                             <  2:1263722/101‑1 (MQ=255)
                                                                     ccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGc                            >  2:2882949/1‑101 (MQ=255)
                                                                      cggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGcc                           >  2:3864439/1‑100 (MQ=255)
                                                                      cggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCg                           <  2:2224124/101‑1 (MQ=255)
                                                                       ggccctCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                          <  1:1001551/101‑1 (MQ=255)
                                                                       ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                          >  1:4650629/1‑101 (MQ=255)
                                                                       ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                          >  1:401136/1‑101 (MQ=255)
                                                                       ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                          >  1:2332542/1‑101 (MQ=255)
                                                                         cccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGc                        <  1:3197609/101‑1 (MQ=255)
                                                                          ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                       <  2:4893307/101‑1 (MQ=255)
                                                                          ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                       <  2:7091118/101‑1 (MQ=255)
                                                                           cgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCg                      <  1:5187400/101‑1 (MQ=255)
                                                                           cccTTGCCGCCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCg                      <  2:348601/99‑1 (MQ=255)
                                                                              ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                   >  1:1736427/1‑101 (MQ=255)
                                                                               tGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCg                  >  1:3428518/1‑101 (MQ=255)
                                                                               tGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCg                  >  2:2972725/1‑101 (MQ=255)
                                                                                 ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                <  1:425814/101‑1 (MQ=255)
                                                                                    aCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGc             <  1:5765630/101‑1 (MQ=255)
                                                                                      cgcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCtt           >  2:5923917/1‑101 (MQ=255)
                                                                                       gcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCttt          >  1:3964621/1‑101 (MQ=255)
                                                                                        ctaTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCGGGATC.GCCCCGGCGGAAATATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  2:3279221/99‑1 (MQ=255)
                                                                                        cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  2:6668120/101‑1 (MQ=255)
                                                                                        cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  1:292199/101‑1 (MQ=255)
                                                                                         gATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCa        <  1:650824/101‑1 (MQ=255)
                                                                                         gATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCa        <  1:1632717/101‑1 (MQ=255)
                                                                                         gATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCa        >  2:3056820/1‑101 (MQ=255)
                                                                                         gATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCa        >  2:4376268/1‑101 (MQ=255)
                                                                                           tGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGc      >  2:6744520/1‑101 (MQ=255)
                                                                                           tGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGc      >  1:6197993/1‑101 (MQ=255)
                                                                                             aGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt    >  1:205360/1‑101 (MQ=255)
                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc   <  2:6120862/101‑1 (MQ=255)
                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc   >  1:2156761/1‑101 (MQ=255)
                                                                                               ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  <  2:6039549/101‑1 (MQ=255)
                                                                                                    |                                                                                                  
ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG