BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,358	Δ1 bp	2.2%	intergenic (‑154/‑151)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,358	0	G→.	2.2%	14.4 ‑0.3 ‑0.3	371	intergenic (‑154/‑151)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (5/3): ref base (191/172): total (196/175)
Fisher's exact test strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.17e-01

Only 100 of 407 total aligned reads displayed.


CCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACC  >  NC_014034/2249259‑2249457


ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                                                       <  1:6379156/101‑1 (MQ=255)


 cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                                                                      <  1:6911051/101‑1 (MQ=255)


       aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                >  1:2063564/1‑101 (MQ=255)


       aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                >  1:5442099/1‑101 (MQ=255)


        agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                               >  2:2681487/1‑101 (MQ=255)


        agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                               <  1:366746/101‑1 (MQ=255)


         gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                              <  1:3082853/101‑1 (MQ=255)


          aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                                             >  1:915172/1‑101 (MQ=255)


           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                                            >  2:5702431/1‑101 (MQ=255)


           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATg                                                                                            >  1:3507498/1‑101 (MQ=255)


            gggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg                                                                                           <  1:6031598/101‑1 (MQ=255)


             ggggCTCTGCCCCCGTCCCGTGCCGGGGCTTCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                                          <  1:5498119/101‑1 (MQ=37)


             ggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                                          >  2:17517/1‑101 (MQ=255)


             ggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                                          >  1:6242450/1‑101 (MQ=255)


              gggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                                         >  2:293063/1‑101 (MQ=255)


              gggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                                         <  1:1009599/101‑1 (MQ=255)


               ggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGTGGTGAATGgcgc                                                                                        <  1:5131447/101‑1 (MQ=255)


                 ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                                                                      >  2:1947672/1‑101 (MQ=255)


                 ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                                                                      <  1:3419422/101‑1 (MQ=255)


                 ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAg                                                                                      >  2:5384173/1‑101 (MQ=255)


                  ctgg.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                                                    >  1:5598807/4‑101 (MQ=255)


                   ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                                                    <  2:5213029/97‑1 (MQ=255)


                   ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAATGGCGCAGcc                                                                                    >  2:4387284/5‑101 (MQ=255)


                    tgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                                                                                   <  1:2984073/98‑1 (MQ=255)


                    tgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                                                                                   <  2:1047666/98‑1 (MQ=255)


                     gaccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                                                  >  2:2783494/3‑101 (MQ=255)


                      cccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                                                                                 <  1:5260297/100‑1 (MQ=255)


                        cccgtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                                                                               >  2:3838024/1‑101 (MQ=255)


                          cctcccggGCCGGGGCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                                                                             <  2:6499042/99‑1 (MQ=255)


                            tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                                                           <  2:4114806/101‑1 (MQ=255)


                             cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                          <  1:1153704/101‑1 (MQ=255)


                             cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                          >  1:1399096/1‑101 (MQ=255)


                             cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                          >  2:5818469/1‑101 (MQ=255)


                               cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                                                                        >  2:1339030/1‑101 (MQ=255)


                                 tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                                                                      <  2:684197/101‑1 (MQ=255)


                                  gCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg.c                                                                    >  2:4909331/1‑101 (MQ=255)


                                     gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccc                                                                >  2:1144738/1‑101 (MQ=255)


                                     gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccc                                                                <  2:5556588/101‑1 (MQ=255)


                                      ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                               <  2:1092191/101‑1 (MQ=255)


                                      ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                               <  1:1228434/101‑1 (MQ=255)


                                         cTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccctt                                                            <  1:2198269/101‑4 (MQ=255)


                                         cTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccctt                                                            <  2:1236503/101‑4 (MQ=255)


                                         cTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccctt                                                            <  2:603262/101‑4 (MQ=255)


                                         cTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccctt                                                            <  1:741631/101‑4 (MQ=255)


                                           cccccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccccttcg                                                          <  2:2863072/101‑6 (MQ=255)


                                            ccccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTcgc                                                          <  2:3106532/101‑1 (MQ=255)


                                               cGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcggg                                                       >  2:4408840/1‑101 (MQ=255)


                                                 ggATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggcg                                                     >  2:4029409/1‑101 (MQ=255)


                                                  gATATTTGAGCCAAGATGAAGGGGGGCTTTCAGCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggcgg                                                    <  2:717367/101‑1 (MQ=25)


                                                    tatTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                  >  2:5920788/1‑101 (MQ=255)


                                                      tttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCTCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                >  1:7068660/1‑101 (MQ=37)


                                                      tttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                >  2:221725/1‑101 (MQ=255)


                                                       ttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgcg                                               >  2:1572422/1‑101 (MQ=255)


                                                         gAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAc                                             <  1:5047355/101‑1 (MQ=255)


                                                         gAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAc                                             >  2:5442851/1‑101 (MQ=255)


                                                          aGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                            <  2:3179761/101‑1 (MQ=255)


                                                          aGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                            >  2:1500299/1‑101 (MQ=255)


                                                           ggCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgc                                           <  1:2935905/101‑1 (MQ=255)


                                                               aGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACgcgcgc                                       >  1:664077/1‑101 (MQ=255)


                                                                gATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                      >  2:6157799/1‑101 (MQ=255)


                                                                gATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                      >  2:2428170/1‑101 (MQ=255)


                                                                 aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                     >  2:833895/1‑101 (MQ=255)


                                                                 aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccc                                     >  2:3047794/1‑100 (MQ=255)


                                                                 aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGAGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                     >  1:1486584/1‑101 (MQ=255)


                                                                   gAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                   <  1:6134862/101‑1 (MQ=255)


                                                                   gAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                   >  1:2396561/1‑101 (MQ=255)


                                                                    aaGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCCTCGCGGGCGGCCGCGCCGCGGGCCGCaa                                  >  2:4330687/1‑101 (MQ=25)


                                                                      ggggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                <  1:2146792/101‑1 (MQ=255)


                                                                          ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                            <  2:2100716/101‑1 (MQ=255)


                                                                           gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                           >  2:6976694/1‑101 (MQ=255)


                                                                            cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                          >  1:4157106/1‑101 (MQ=255)


                                                                             tttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                         >  1:6683171/1‑101 (MQ=255)


                                                                              ttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                        >  1:5823355/1‑101 (MQ=255)


                                                                              ttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                        >  1:3966207/1‑101 (MQ=255)


                                                                               tCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                       >  2:2562612/1‑101 (MQ=255)


                                                                               tCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                       >  2:6812935/1‑101 (MQ=255)


                                                                                cATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                      <  1:1769225/101‑1 (MQ=255)


                                                                                cATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                      >  1:6766337/1‑101 (MQ=255)


                                                                                cATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                      >  1:3615629/1‑101 (MQ=255)


                                                                                 aTCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                     <  1:2759726/101‑1 (MQ=255)


                                                                                  tCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGGCGCGACGCGCGCCGCCACAGGTCGTCCCCCt                    >  1:4716913/1‑101 (MQ=25)


                                                                                   cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                   >  1:4331909/1‑101 (MQ=255)


                                                                                   cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                   >  1:4846329/1‑101 (MQ=255)


                                                                                   cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                   >  1:1091007/1‑101 (MQ=255)


                                                                                   cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCtt                   >  1:3269017/1‑101 (MQ=255)


                                                                                    ttGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                  <  1:3351043/101‑1 (MQ=255)


                                                                                      gcagAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                <  1:4480838/98‑1 (MQ=255)


                                                                                       ccGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc               <  2:3719750/101‑1 (MQ=255)


                                                                                       ccGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc               <  1:6075782/101‑1 (MQ=255)


                                                                                       ccGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc               <  2:6699401/101‑1 (MQ=255)


                                                                                         gAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt             <  1:3838187/101‑1 (MQ=255)


                                                                                          aaaaTATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt            <  1:1924798/101‑1 (MQ=255)


                                                                                           aaaTATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc           >  1:3764805/1‑101 (MQ=255)


                                                                                            aaTATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt          >  2:4712624/1‑101 (MQ=255)


                                                                                            aaTAGCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt          <  1:1354500/101‑1 (MQ=255)


                                                                                               atCCCGGGGGGTGAATGGCGCAGCCAGAGGGGTGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc       >  1:1620050/1‑101 (MQ=255)


                                                                                               atCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc       >  2:3488088/1‑101 (MQ=255)


                                                                                                   cGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CGCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc   >  2:6228787/1‑101 (MQ=255)


                                                                                                    ggggggTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc  >  2:6345672/1‑101 (MQ=255)


                                                                                                    ggggggTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc  >  2:3932496/1‑101 (MQ=255)


CCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACC  >  NC_014034/2249259‑2249457

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG