Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,316,712 Δ1 bp 99.0% intergenic (‑20/‑245) RCAP_rcc02149 ← / → acnA hypothetical protein/aconitate hydratase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,316,7120A→.99.0% 4.4 ‑0.5 ‑0.3303intergenic (‑20/‑245)RCAP_rcc02149/acnAhypothetical protein/aconitate hydratase
Reads supporting (aligned to +/- strand):  new base (173/127):  ref base (1/2):  total (174/129)
Fisher's exact test strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.17e-01

Only 100 of 315 total aligned reads displayed.

GCCTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCCCGGCGCTGAAGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGATGCCTG  >  NC_014034/2316612‑2316808
                                                                                                    |                                                                                                
gCCTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTGa                                                                                                  <  1:5394030/101‑1 (MQ=255)
gCCTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTGa                                                                                                  <  2:4730084/101‑1 (MQ=255)
  cTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTGagg                                                                                                >  1:5855118/1‑99 (MQ=255)
  cTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTGagg                                                                                                >  2:2520850/1‑99 (MQ=255)
  cTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTGagg                                                                                                >  2:2723427/1‑99 (MQ=255)
     cgcCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACTGGATCATGCCTTCGATCGCGGCGCTG.AGGGTc                                                                                            >  2:3901591/1‑101 (MQ=255)
     cgcCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTc                                                                                            >  2:2188316/1‑101 (MQ=255)
      gcCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcg                                                                                           >  1:4545217/1‑101 (MQ=255)
       ccAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcgc                                                                                          <  1:5991329/101‑1 (MQ=255)
        cAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcgcg                                                                                         >  1:2114096/1‑101 (MQ=255)
         aaaGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcgcgc                                                                                        >  2:82521/1‑101 (MQ=255)
          aaGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcgcgcg                                                                                       <  2:355675/101‑1 (MQ=255)
           aGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGt                                                                                      >  2:5552978/1‑101 (MQ=255)
             ccccccGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTcgcgggggc                                                                                    >  1:2269182/1‑96 (MQ=255)
                cccGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCat                                                                                 <  2:1180510/101‑1 (MQ=255)
                 ccGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCatg                                                                                <  1:4300689/101‑1 (MQ=255)
                  cGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCatga                                                                               >  1:3254151/1‑101 (MQ=255)
                   gTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCatgat                                                                              >  1:2859615/1‑101 (MQ=255)
                     cGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGc                                                                            >  2:602399/1‑101 (MQ=255)
                      ggTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGcc                                                                           >  1:3615609/1‑101 (MQ=255)
                      ggTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGcc                                                                           >  2:5391259/1‑101 (MQ=255)
                         cctgcctgCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCg                                                                        >  2:6539216/1‑101 (MQ=255)
                          ctgcctgcCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGc                                                                       >  1:5880558/1‑101 (MQ=255)
                           tgcctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGct                                                                      <  1:4742738/101‑1 (MQ=255)
                            gcctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGctc                                                                     >  2:5736272/1‑101 (MQ=255)
                            gcctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGctc                                                                     >  1:2926163/1‑101 (MQ=255)
                             cctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGctct                                                                    <  2:3990833/101‑1 (MQ=255)
                              ctggcCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCtt                                                                   >  1:5615932/1‑101 (MQ=255)
                              ctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCtt                                                                   >  2:149359/1‑101 (MQ=255)
                              ctgccCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCtt                                                                   <  1:934263/101‑1 (MQ=255)
                                 cccccATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACt                                                                <  2:6383196/101‑1 (MQ=255)
                                 cccccATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACt                                                                <  1:6601149/101‑1 (MQ=255)
                                  ccccATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTg                                                               <  2:2967341/101‑1 (MQ=255)
                                   ctcATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGc                                                              <  2:3806459/99‑1 (MQ=255)
                                    ccATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCa                                                             >  2:4088992/1‑101 (MQ=255)
                                     cATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAg                                                            >  1:1767719/1‑101 (MQ=255)
                                     cATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAg                                                            >  1:3806620/1‑101 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         <  1:2678635/101‑1 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         >  1:6997073/1‑101 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         >  1:6496193/1‑101 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         >  1:1521248/1‑101 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         >  1:4681663/1‑101 (MQ=255)
                                        gCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGccc                                                         >  1:549987/1‑101 (MQ=255)
                                            cgaTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcct                                                     <  2:5990850/99‑1 (MQ=255)
                                            ccaTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcct                                                     <  2:2864756/101‑1 (MQ=255)
                                            ccaTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcct                                                     <  1:343218/101‑1 (MQ=255)
                                             caTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcctc                                                    <  1:5245367/101‑1 (MQ=255)
                                              aTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcctcc                                                   >  2:6162845/1‑101 (MQ=255)
                                              aTGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcctcc                                                   >  1:2590072/1‑101 (MQ=255)
                                               tGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCcctcct                                                  <  2:4644041/101‑1 (MQ=255)
                                                gggAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTa                                                 <  1:2510361/101‑1 (MQ=255)
                                                 ggAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTaa                                                >  2:5691127/1‑101 (MQ=255)
                                                   aGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAAc                                              >  2:2421726/1‑101 (MQ=255)
                                                   aGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAAc                                              >  2:6428450/1‑101 (MQ=255)
                                                    gCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAAcc                                             <  2:6646794/101‑1 (MQ=255)
                                                     cAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCg                                            <  2:2074607/101‑1 (MQ=255)
                                                     cAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCg                                            >  1:1868214/1‑101 (MQ=255)
                                                      aaCGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCgg                                           >  1:6662374/1‑101 (MQ=255)
                                                      aaCGCCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCgg                                           >  2:1914401/1‑101 (MQ=255)
                                                         ggCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGgcgc                                        >  1:370357/1‑101 (MQ=255)
                                                         ggCAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGgcgc                                        >  1:3973593/1‑101 (MQ=255)
                                                           cAAGAAAGCCCGCCAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGTCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGcgccg                                      >  1:5096758/1‑101 (MQ=255)
                                                           cAAGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGcgccg                                      >  1:4977572/1‑101 (MQ=255)
                                                            aaGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGGTGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGcgccgc                                     >  1:1347020/1‑101 (MQ=255)
                                                            aaGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGcgccgc                                     >  1:1952730/1‑101 (MQ=255)
                                                            aaGAAAGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGcgccgc                                     <  2:4337327/101‑1 (MQ=255)
                                                                aaGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCgcgcgc                                 >  1:1017977/1‑101 (MQ=255)
                                                                aaGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCgcgagc                                 >  1:839957/1‑101 (MQ=255)
                                                                 aGCCCGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCa                                >  1:2545233/1‑101 (MQ=255)
                                                                     cGACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGc                            >  2:2471487/1‑101 (MQ=255)
                                                                      gACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGcc                           <  2:5876166/101‑1 (MQ=255)
                                                                      gACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGcc                           >  1:3071394/1‑101 (MQ=255)
                                                                      gACAGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGcc                           >  2:4155597/1‑101 (MQ=255)
                                                                         aGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAAt                        <  1:118477/101‑1 (MQ=255)
                                                                         aGGATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAAt                        <  2:6321854/101‑1 (MQ=255)
                                                                          ggATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATc                       <  2:181078/101‑1 (MQ=255)
                                                                           gATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCa                      >  1:2418916/1‑101 (MQ=255)
                                                                           gATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCa                      >  1:6186223/1‑101 (MQ=255)
                                                                           gATCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCACCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCa                      >  2:1982518/1‑101 (MQ=255)
                                                                            aTCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCCCCTAAACCGGCGCCGCGCGCAGGGCCAATCaa                     <  1:6044908/101‑1 (MQ=255)
                                                                             tCATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAg                    >  2:3638742/1‑101 (MQ=255)
                                                                             tCATCCCTTCAATCGCGGCTC.GTAGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAg                    <  2:3329815/101‑1 (MQ=255)
                                                                              cATGCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAgg                   <  1:6428780/101‑1 (MQ=255)
                                                                                 gCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGttt                <  2:7089246/101‑1 (MQ=255)
                                                                                 gCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGttt                >  2:4795553/1‑101 (MQ=255)
                                                                                 gCCTTCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGttt                >  2:2788201/1‑101 (MQ=255)
                                                                                    ttCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGc             >  1:866234/1‑101 (MQ=255)
                                                                                     tCAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCg            >  2:5231385/1‑101 (MQ=255)
                                                                                      cAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCTATCAAGGTTTTGCga           <  1:6871443/101‑1 (MQ=255)
                                                                                      cAATCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCga           <  2:3254035/101‑1 (MQ=255)
                                                                                       aaTCGCGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCgat          >  1:5916494/1‑101 (MQ=255)
                                                                                           gcGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGatgatg      >  2:5142461/2‑101 (MQ=255)
                                                                                           gcGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGatgatg      <  2:2587220/100‑1 (MQ=255)
                                                                                           gcGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGatgatg      <  2:3467366/100‑1 (MQ=255)
                                                                                            cGGCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgc     <  1:7063352/101‑1 (MQ=255)
                                                                                             ggCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgcc    >  1:3911743/1‑101 (MQ=255)
                                                                                             ggCGCTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgcc    >  2:6854959/1‑101 (MQ=255)
                                                                                              gcgcTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgcct   <  2:6996699/101‑1 (MQ=255)
                                                                                               cgcTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgcctg  <  1:332937/101‑1 (MQ=255)
                                                                                               cgcTG.AGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGAtgcctg  >  2:1568844/1‑101 (MQ=255)
                                                                                                    |                                                                                                
GCCTGCGCCAAAGCCCCCCGTCGGTCCTGCCTGCCCCCATGCCACCATGGGAGCAACGGCAAGAAAGCCCGACAGGATCATGCCTTCAATCCCGGCGCTGAAGGGTCGCGCGTGCCATGATGCCTCGCTCTTACTGCAGCCCCCCTCCTAAACCGGCGCCGCGCGCAGGGCCAATCAAGGTTTTGCGATGATGCCTG  >  NC_014034/2316612‑2316808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG