Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	227.2	65	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (52/13):  ref base (0/0):  total (52/13)

GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGTG  >  NC_014034/2474814‑2475010                                                                                                     |                                                                                                         gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGGCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                          >  2:3453863/1‑101 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                          <  2:2945364/101‑1 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                          <  2:4863202/101‑1 (MQ=255)   tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggg                                                                                                        <  2:3745382/101‑1 (MQ=255)   tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggg                                                                                                        <  1:2788124/101‑1 (MQ=255)   tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggg                                                                                                        <  2:4053198/101‑1 (MQ=255)     ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                                      <  2:485385/101‑1 (MQ=255)      cccGGGATATTTCCCCCAAGGTGACAGGTAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                                     <  1:4974757/101‑1 (MQ=255)      cccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                                     <  1:1052747/101‑1 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                                    <  1:2602957/101‑2 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                                    <  1:2955961/101‑2 (MQ=255)          ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                                 <  2:5178986/101‑5 (MQ=255)          ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                                 <  1:6893772/101‑5 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAAGCGGGGGGTGC.GGGGGGCGCAg                                                                                               >  1:3665278/1‑101 (MQ=255)             tatTTCCCCCAAGGTGACAGGAACCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                             <  2:4510815/101‑1 (MQ=255)             tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                             <  2:2066554/101‑1 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCCAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                            >  1:2766808/1‑101 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                            <  2:5925235/101‑1 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                            <  1:1089123/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                           <  1:6378729/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                           >  2:5975819/1‑101 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.c                                                                                         <  1:558148/101‑1 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCACcccc.c                                                                                         <  1:4234350/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.cc                                                                                        <  1:3255032/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.cc                                                                                        <  2:1873334/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.cc                                                                                        <  2:1784571/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCACACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.cc                                                                                        <  1:3407298/101‑1 (MQ=255)                 tCCCCAAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc.cc                                                                                        <  1:4696861/101‑1 (MQ=255)                     ccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.gg                                                                               >  1:1976459/1‑101 (MQ=255)                      cAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.ggg                                                                              >  2:3080727/1‑101 (MQ=255)                           tGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGG.GCAGcccc.ccacccggcgcc                                                                              >  2:720792/1‑91 (MQ=255)                           tGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.ggggcccc                                                                         >  1:4012748/1‑96 (MQ=255)                                 gAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGGCC.CGGGc                                                                  >  2:2088970/1‑101 (MQ=37)                                      cgcgGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCC.CGGGGCTGcc                                                             >  1:1289545/1‑101 (MQ=25)                                       gcgGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CCGGGCTGccc                                                            >  1:745172/1‑101 (MQ=25)                                         ggCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCCTGccccg                                                          >  2:6205525/1‑99 (MQ=25)                                         ggCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCTCCGGGCTGGCCCg                                                           >  2:4910664/1‑101 (MQ=25)                                             ccccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCCTGCCCGGgggg                                                      >  1:1692870/1‑99 (MQ=21)                                                 ccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCC.CCGGCGTGCCCCAgggggccc                                                  >  1:5178367/1‑95 (MQ=21)                                                   aCCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCC.CGGGCTTGCCCGCGGCggccacc                                                >  1:2814735/1‑95 (MQ=21)                                                       gAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCCTGCCCGCGGCGGCATCAG.ccc                                           >  2:808852/1‑101 (MQ=21)                                                        aaCGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCTTGCCCGGGGGGGCATCCG.cccc                                          >  1:4473061/1‑100 (MQ=21)                                                          cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGGGCCC.CGGGGGTGCCCGCGGGGGCCCCAG.cccccc                                        >  1:1442914/1‑98 (MQ=16)                                                          cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGGCT.CCGGGGTGCGCGCGGCCAGATCAG.CCCTTc                                        >  2:1916047/1‑101 (MQ=21)                                                          cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCCGCC.CGGGCTTGCCCGCGGCGAAATCAG.CCCCTc                                        >  2:847550/1‑101 (MQ=21)                                                           ggCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCCTGCCCGAGGCGACATCCG.CCCCTCg                                       >  1:4355539/1‑101 (MQ=18)                                                           ggCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGACT.CGGGCTTGCCCGAGGGGACAACAG.CCCTTCg                                       >  2:2946672/1‑101 (MQ=25)                                                              ccGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCCG.CCCCTccggt                                    >  1:6986313/1‑97 (MQ=21)                                                                    tttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCTTGCCCGCGGCGACATCAG.CCCCTCCGGTTCGAgg                              >  2:3043193/1‑101 (MQ=21)                                                                     ttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCCTGCCCGAGGCGACATCAG.CCCCTCGGTTTCGAggg                             >  1:2650515/1‑101 (MQ=21)                                                                       ccTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CCGGCTTGCCCCGGGCGACATCAG.GCCCTCGGTTTcccgagca                           >  2:6023531/1‑94 (MQ=21)                                                                         ttGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCCTGCCCGCGGCGGCAACAG.CCCCTCGGGTTCGGGGGGAgg                         >  2:3109429/1‑101 (MQ=18)                                                                          tGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GCGGGGGCGCCC.CGGGCTTGCCCGGGGGGGCACCAG.CCCTCCGGTTTTGGGGGCGgg                         >  1:1479691/1‑101 (MQ=16)                                                                          tGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGGGGCGACATCAG.CCCTTCGGGTTCGAGGGCAGGc                        >  1:3008980/1‑101 (MQ=255)                                                                            gCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCCTCGGGTTCGAGGGCAGGccc                      >  1:2757907/1‑101 (MQ=255)                                                                             ccAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCGT.CGGGCTTGCCCGAGGCGACATCAG.CCCCTCGGGTTCGAGGGCAGGCCCt                     >  1:4204384/1‑101 (MQ=25)                                                                             ccAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCGTTCGCCAGGCGACAACCG.CCCTTCGGGCTCGTggggctgcccc                     >  1:395560/1‑93 (MQ=12)                                                                              cAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGCCCGGGGCGACATCAGCCCCTCCGGTTTCG.GGGCAGGcccct                    >  2:1171274/1‑99 (MQ=18)                                                                               aaaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGCCCGGGGCGCCATCAG.CCCTTCGGGTTTGAGGGCAGGCCCTTg                   >  1:808585/1‑101 (MQ=21)                                                                                 aTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCCTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCGTGTc                 >  2:1181566/1‑101 (MQ=255)                                                                                  tACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCG.CC....GC.GGGGCGCCT.CGGGGTTGCCCCAGGGGGCATCAG.CCCTTCGGGTTCGAGGGCGGGCCCCTGTcc                >  1:6373556/1‑101 (MQ=21)                                                                                  tACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGCCCGGGGGGACCTCAG.CCCTTCGGGTTCCGGGGCGGCCCCTTGCcc                >  1:537372/1‑101 (MQ=18)                                                                                   ggcggggggggggggGGGGGGGCCGCCCCGCCCCGCG....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGTGGGCAGGCCTTTGTc                 <  1:5636201/92‑1 (MQ=16)                                                                                   gcggggggCGGGGGGGGCGGGGCGGCGCCCCCCC.CCCCGGGC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCtt                    <  2:2650419/95‑1 (MQ=21)                                                                                   aCGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGGTTCGAGGGCAGGGCCCTGTCCt               >  2:1951215/1‑101 (MQ=21)                                                                                   aCGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGGCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCt               >  1:1303477/1‑101 (MQ=255)                                                                                    gcggggggggggggCGGGGGGGGGCCCCCCCCC.CCCG..GC.GGGTCGCCT.CGGGCTTGCCGGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTc                 <  2:3443052/93‑1 (MQ=21)                                                                                    cGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGGGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCtt              >  1:1279538/1‑101 (MQ=255)                                                                                    cGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGGGGGCAGGCCCCTGTCCtt              >  1:683001/1‑101 (MQ=25)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGGTTGCCCGAGGCGACATCAG.GCCTTCGGGTTCGAGGGCAGGGCCTTGTCCTttcg           >  2:2672869/1‑101 (MQ=21)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTtgcg           <  2:745148/101‑1 (MQ=255)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCCTCGGTTTCGAGGGCAGGCCCTTGTCCTtgcg           >  2:6202922/1‑101 (MQ=255)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGGTTCGAGGGCAGGGCCTTGTCCTtgcc           >  1:1914828/1‑100 (MQ=21)                                                                                         ggCGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGAAATCAG.CCCTTCGGGTTCGCGGGCCGGGCCTTGTcccttccag         >  2:4633462/1‑94 (MQ=17)                                                                                           cGGGGGGTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGGCATCAG.CCCTTCGGGTTCGAGGGCAGGCCCTTGTCCTtgcgtgcg       >  2:1575915/1‑101 (MQ=255)                                                                                            ggggggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTgcgg      >  1:3083752/1‑101 (MQ=255)                                                                                            ggggggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCC.CGGGCTTGCCCGAGGCGGCCTCAG.CCCTGCGGGTTCGAGGGGAGGCCCTTGTCCTTGCGTgcgg      >  1:3381372/1‑101 (MQ=18)                                                                                            ggggggGGGGGGGGGCCCCCCCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTtgcgtgcg       <  2:1289497/101‑1 (MQ=37)                                                                                            ggggggGGGGGGGGCGCCCCCCCCC.CT....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTtgcgtgcg       <  2:2766706/101‑1 (MQ=37)                                                                                             gggggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGGgcggg     >  1:6452117/1‑100 (MQ=255)                                                                                             gggggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGGCGCCC.CGGGGCTGCCCGAGGCGACATCAG.CCCCTCCGTTTCGCGGGCCGGGCCTTGGCCCTGCGTgcggg     >  2:5242893/1‑100 (MQ=12)                                                                                              ggggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTgcggcg    >  1:6378955/1‑101 (MQ=255)                                                                                               gggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTgcggcgg   >  2:3188594/1‑100 (MQ=255)                                                                                               gggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGAGCCATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTGCTTGCGTgcggcgg   >  2:1674313/1‑100 (MQ=25)                                                                                                ggTGC.GGGGGGCGCAGCCCC.CC....GC.GGGTCGCCC.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGGCCTTGTCCTTGCGTgcggcggg  >  2:1552335/1‑99 (MQ=25)                                                                                                     |                                                                                                         GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCC.CC....GC.GGGTCGCCT.CGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGTG  >  NC_014034/2474814‑2475010 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG