Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,047 Δ1 bp 4.6% intergenic (+95/‑147) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0470G→. 4.6% 28.2 0.0 ‑0.0262intergenic (+95/‑147)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (132/118):  total (138/124)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.70e-01

Only 100 of 302 total aligned reads displayed.

AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146
                                                                                                    |                                                                                                           
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             <  1:1425872/101‑1 (MQ=18)
 gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                            >  2:5005216/6‑101 (MQ=255)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATCTTTGCAGCAAGATGAAACCGGATGCCCTTCATCTTGCCCTAAATATCCCggg                                                                                                           >  1:2370275/5‑101 (MQ=18)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                           >  2:2995194/5‑101 (MQ=255)
    aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCggggg                                                                                                         >  1:1715591/3‑101 (MQ=255)
     ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATACCCCgggggg                                                                                                        <  1:6828304/100‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:1622951/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:6943153/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:1978697/101‑1 (MQ=255)
        gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                     >  2:4706240/1‑101 (MQ=255)
         ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                                                    >  2:4137507/1‑101 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  <  1:3404206/101‑1 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  <  1:2840836/101‑1 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  <  2:3657013/101‑1 (MQ=255)
           ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  2:530791/101‑1 (MQ=255)
            gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                <  2:6960075/101‑1 (MQ=255)
            gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                <  1:3696420/101‑1 (MQ=255)
             cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.c                                                                                              >  2:1581032/1‑101 (MQ=255)
             cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.c                                                                                              >  1:6622282/1‑101 (MQ=255)
               ctccccccCGTCCGCAAGCCGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.a                                                                                         <  1:848607/98‑1 (MQ=18)
                tccccccCGTCCGAAAGCGGCCT.CCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aag                                                                                       <  1:4904669/99‑3 (MQ=18)
                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aag                                                                                       <  1:4131707/101‑3 (MQ=255)
                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aagccag                                                                                   >  2:1717738/5‑95 (MQ=255)
                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aagccaga                                                                                  <  1:5334996/98‑8 (MQ=255)
                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aagccaga                                                                                  <  2:1889109/98‑8 (MQ=255)
                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.aagccaga                                                                                  <  1:2421694/98‑8 (MQ=255)
                                gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagc                                                                        <  1:3524300/101‑3 (MQ=18)
                                gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagc                                                                        >  2:5420299/1‑99 (MQ=18)
                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcg                                                                       >  2:4103598/1‑98 (MQ=18)
                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcg                                                                       >  2:4364566/1‑98 (MQ=18)
                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcg                                                                       >  2:5775738/1‑98 (MQ=18)
                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCagcgc                                                                      >  2:7107447/1‑97 (MQ=18)
                                   gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                   >  1:2701939/1‑101 (MQ=18)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                 >  2:6585192/1‑101 (MQ=18)
                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cc                                                                >  2:4049367/1‑101 (MQ=18)
                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                              >  1:222273/1‑100 (MQ=17)
                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGG.AGGCG.cc.cccc                                                              >  2:2902251/1‑100 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cccct                                                             <  2:3617111/101‑3 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cccct                                                             <  2:3212537/101‑3 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cccca                                                             <  2:5732257/101‑3 (MQ=17)
                                           ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctgcc                                                          <  1:994628/101‑6 (MQ=17)
                                            cGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCAGCGG.CC.CCCTGccc                                                          >  2:3513234/1‑101 (MQ=18)
                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                         >  1:6228942/1‑101 (MQ=0)
                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                         >  2:1766453/1‑101 (MQ=17)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                        <  2:6340765/101‑1 (MQ=0)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                        <  2:5653150/101‑1 (MQ=17)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                        >  2:3475203/1‑101 (MQ=18)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                        >  1:5969404/1‑101 (MQ=18)
                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                       >  1:6081322/1‑100 (MQ=2)
                                               gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                       >  2:31145/1‑101 (MQ=18)
                                                atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                                                      <  2:2034199/101‑1 (MQ=17)
                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgccg                                                     >  2:3164905/1‑101 (MQ=17)
                                                    ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                  <  2:6476340/101‑3 (MQ=2)
                                                    ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGG.CCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                  <  2:5078699/101‑3 (MQ=2)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                 <  1:707982/101‑1 (MQ=17)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGG.C..GAAGCCAGAGGGGGGACGGGC..CC.CCCGGCCCCGGCGGTcc                                                 >  2:4252375/1‑101 (MQ=17)
                                                      gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                                                <  1:315144/101‑1 (MQ=17)
                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                             >  1:4493287/1‑101 (MQ=18)
                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                          >  1:3811002/1‑101 (MQ=17)
                                                             gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGGCCGGggcggc                                         >  1:3877195/1‑101 (MQ=17)
                                                               tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.A..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                       >  2:4546162/1‑101 (MQ=18)
                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGcc                                      >  1:334723/1‑101 (MQ=18)
                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGcc                                      >  1:3054694/1‑101 (MQ=17)
                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGcc                                      >  1:2865893/1‑101 (MQ=17)
                                                                  aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGACC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                    >  2:4633550/1‑101 (MQ=18)
                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  2:1348192/101‑1 (MQ=17)
                                                                    ccGGATGCTTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                  <  2:3555338/101‑1 (MQ=17)
                                                                     cGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGat                                 <  1:4085202/101‑2 (MQ=17)
                                                                      ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                >  2:3847130/1‑101 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                              <  2:2701843/101‑1 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                              >  2:6118784/1‑101 (MQ=18)
                                                                         tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                             <  1:1574503/101‑1 (MQ=17)
                                                                             tttGATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCCGCCCCGGCGGTCCGTGTCGGCGGCTTGACGGGCgggg                         >  1:5613427/1‑101 (MQ=9)
                                                                              ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGCAGCTC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                        <  1:1009584/101‑1 (MQ=18)
                                                                              ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                        <  1:1828257/101‑1 (MQ=18)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCC..A.AG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       >  2:2498333/1‑101 (MQ=18)
                                                                               tCATCTGGCCCGAGATGTCGCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       <  1:6428046/101‑1 (MQ=16)
                                                                               tCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       <  1:3608431/101‑1 (MQ=18)
                                                                                cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGG.AAGCGCCC.CCCTGCCCCGGCGGCCCGTGGCGGCGCCTTGACGGGCGGGGgcg                      >  2:4780192/1‑101 (MQ=18)
                                                                                 aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                     <  1:4211456/101‑1 (MQ=18)
                                                                                  tcgtGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    <  2:1837078/98‑1 (MQ=18)
                                                                                  tCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    <  2:6181575/101‑1 (MQ=18)
                                                                                  tCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcccg                    >  1:6141678/1‑101 (MQ=18)
                                                                                   cTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                   <  2:1170636/101‑1 (MQ=18)
                                                                                    ttGCCCGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                  >  1:4032261/1‑101 (MQ=18)
                                                                                     tgaccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCG.CCACCCTGCCCCGGAGGAAAGTGAAGGCGCCTTGTCGGGCGGGGGGGCGGGc                 >  1:3451881/4‑101 (MQ=2)
                                                                                       cccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt               <  1:4732130/101‑1 (MQ=18)
                                                                                        ccGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  2:6003847/101‑1 (MQ=18)
                                                                                        ccGAAATATCCCGGGGGGAGAA.TGG.C..GAAG.CA.AGGGGGGGGCAGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  1:1136679/101‑1 (MQ=21)
                                                                                         cGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa             <  1:5881703/101‑1 (MQ=18)
                                                                                         cGAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa             <  1:6480476/101‑1 (MQ=18)
                                                                                          gAAATCGCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  2:2631363/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  2:1978730/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            >  2:2468014/1‑101 (MQ=18)
                                                                                           aaaTATCCCGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAAc           >  1:522527/1‑101 (MQ=18)
                                                                                                 cccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGGCCCGGCGGGCCGTGGCGGCGCGTTGGCGGGCGGGGGGGCGGGCGGTAAccagacg     >  2:2270117/1‑96 (MQ=9)
                                                                                                  ccGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt    <  1:6147874/101‑1 (MQ=21)
                                                                                                   cGGGGGGTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg   <  1:6586212/101‑1 (MQ=25)
                                                                                                    ggggggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc  >  1:3245133/1‑101 (MQ=25)
                                                                                                    ggggggTGAA.TGG.C..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc  <  1:4049543/101‑1 (MQ=25)
                                                                                                    |                                                                                                           
AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGG.C..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG