Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,551,310 Δ1 bp 100% intergenic (‑45/+124) RCAP_rcc02379 ← / ← ftsI CobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,551,3100A→.100.0% 1406.6 318intergenic (‑45/+124)RCAP_rcc02379/ftsICobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI
Reads supporting (aligned to +/- strand):  new base (184/134):  ref base (0/0):  total (184/134)

Only 100 of 344 total aligned reads displayed.

AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCGG  >  NC_014034/2551210‑2551406
                                                                                                    |                                                                                                
  aaaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaag                                                                                                >  2:3611639/1‑100 (MQ=255)
     gCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagccc                                                                                             <  2:6006139/101‑5 (MQ=255)
      cccGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccg                                                                                           <  1:740565/101‑1 (MQ=255)
       ccGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgc                                                                                          <  1:1504529/101‑1 (MQ=255)
       ccGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgc                                                                                          <  1:4523235/101‑1 (MQ=255)
       ccGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgc                                                                                          >  2:1116632/1‑101 (MQ=255)
        cGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgcc                                                                                         >  1:4744571/1‑101 (MQ=255)
         gAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgccc                                                                                        >  2:4854141/1‑101 (MQ=255)
         gAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgccc                                                                                        >  2:6853063/1‑101 (MQ=255)
         gAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgccc                                                                                        >  1:3036748/1‑101 (MQ=255)
           aaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccc                                                                                      >  2:5228793/1‑101 (MQ=255)
            aGCACGCTGGCCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccccc                                                                                     <  2:6240606/101‑1 (MQ=255)
             gCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccccc                                                                                    <  1:5900986/101‑1 (MQ=255)
             gCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccccc                                                                                    <  2:6306384/101‑1 (MQ=255)
             gCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccccc                                                                                    <  2:7006218/101‑1 (MQ=255)
             gCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccccc                                                                                    <  2:3008468/101‑1 (MQ=255)
                  cTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAg                                                                               <  2:2397082/101‑1 (MQ=255)
                   tGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGc                                                                              <  1:5664210/101‑1 (MQ=255)
                     aCCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGccc                                                                            >  2:3993315/1‑101 (MQ=255)
                     aCCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGccc                                                                            >  1:207996/1‑101 (MQ=255)
                     aCCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGccc                                                                            <  2:664365/101‑1 (MQ=255)
                        ggAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGcc                                                                         >  1:6116128/1‑101 (MQ=255)
                          aaGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaa                                                                       >  2:4117357/1‑101 (MQ=255)
                           aGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaa                                                                      <  1:494482/101‑1 (MQ=255)
                              cGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagca                                                                   <  1:4281993/101‑1 (MQ=255)
                                 ggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcagag                                                                <  2:325859/101‑1 (MQ=255)
                                  gATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.ACGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAgg                                                               <  1:6371100/101‑1 (MQ=255)
                                  gATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAgg                                                               >  1:5516310/1‑101 (MQ=255)
                                  gATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAgg                                                               <  1:2479753/101‑1 (MQ=255)
                                    tCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGc                                                             <  1:3958209/101‑1 (MQ=255)
                                     cAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGcc                                                            <  1:6746255/101‑1 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCg                                                           <  1:4029335/101‑1 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCg                                                           <  2:3719465/101‑1 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCg                                                           <  1:6194129/101‑1 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                          <  2:5114980/101‑1 (MQ=255)
                                         gggAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCGGCCAAAGCAAAGGGCCGGAc                                                        >  2:6954305/1‑101 (MQ=255)
                                          ggAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAcc                                                       >  2:533805/1‑101 (MQ=255)
                                           gagaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAccc                                                      <  1:1625254/101‑1 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                     <  1:1524906/101‑1 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                    <  1:6589937/101‑1 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                    <  2:727686/101‑1 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                    >  1:1146877/1‑101 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                   >  1:3087840/1‑101 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                   <  2:1522770/101‑1 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAACCCCGCCAAAGCAAAGTGCCGGACCCTGc                                                   <  1:5059804/101‑1 (MQ=255)
                                               aaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGAGACGGACCCTGCg                                                  >  1:2418787/1‑101 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCACGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                               >  1:2252274/1‑101 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                               >  1:6037997/1‑101 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                               >  1:6494774/1‑101 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                              <  1:3745496/101‑1 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                              <  2:4304860/101‑1 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                              >  2:1937978/1‑101 (MQ=255)
                                                    tCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATGGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCg                                             >  2:3387995/1‑101 (MQ=255)
                                                    tCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCg                                             <  1:3411171/101‑1 (MQ=255)
                                                      aTGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAc                                           >  1:786903/1‑101 (MQ=255)
                                                       tGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGTCCCCCCGCACGCCCGCCAAAGCAAAGGGCCGGACCCTGCTGTCCGAcc                                          >  2:473715/1‑101 (MQ=255)
                                                        ggCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccc                                         >  2:5033337/1‑101 (MQ=255)
                                                        ggCCGCCCATCTGAGGTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccc                                         >  1:6866360/1‑101 (MQ=255)
                                                          ccGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccg                                       >  1:5968739/1‑101 (MQ=255)
                                                           cGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgt                                      <  2:4972917/101‑1 (MQ=255)
                                                           cGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgt                                      <  1:3426176/101‑1 (MQ=255)
                                                            gTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtg                                     <  1:908745/101‑1 (MQ=255)
                                                             tCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgt                                    >  1:202816/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                   >  2:5386405/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                   >  1:622733/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                   >  2:3593616/1‑101 (MQ=255)
                                                               cATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttt                                  >  1:3249267/1‑101 (MQ=255)
                                                                aTCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtttt                                 <  1:4583178/101‑1 (MQ=255)
                                                                 tCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttc                                <  2:1578387/101‑1 (MQ=255)
                                                                   tGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccc                              >  2:5533200/1‑101 (MQ=255)
                                                                    gAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttcccc                             <  2:6115816/101‑1 (MQ=255)
                                                                     aGTTATGTTATACTATCACCCAAATAGACCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccg                            >  1:1233632/1‑101 (MQ=255)
                                                                      gttatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccggggtttccccgg                           >  1:6939755/1‑100 (MQ=255)
                                                                        tatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgt                         >  1:4131700/1‑101 (MQ=255)
                                                                         atgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgtt                        >  2:1724930/1‑101 (MQ=255)
                                                                           gttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgtttt                      <  2:2610788/101‑1 (MQ=255)
                                                                            ttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGttttt                     <  2:3087727/101‑1 (MQ=255)
                                                                              ataCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTcc                   >  2:5713826/1‑101 (MQ=255)
                                                                              ataCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTcc                   >  2:646164/1‑101 (MQ=255)
                                                                              ataCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTcc                   <  1:2704602/101‑1 (MQ=255)
                                                                               taCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCt                  >  1:2355989/1‑101 (MQ=255)
                                                                                aCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTg                 >  2:2731073/1‑101 (MQ=255)
                                                                                aCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTg                 <  1:6398393/101‑1 (MQ=255)
                                                                                 cTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTgg                <  2:4480640/101‑1 (MQ=255)
                                                                                    tCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGc             <  1:3611788/101‑1 (MQ=255)
                                                                                    tCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGCGTTTTTCCCGGTGc             >  2:482441/1‑101 (MQ=255)
                                                                                      aCCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaa           <  1:4405830/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAACGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt         <  1:6434363/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt         <  2:790912/101‑1 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        <  2:3862561/101‑1 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        >  1:1759005/1‑101 (MQ=255)
                                                                                         cAAAGAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        <  1:3466921/101‑1 (MQ=255)
                                                                                          aaaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATAc       <  2:1808698/101‑1 (MQ=255)
                                                                                           aaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATaag      >  1:535315/1‑99 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATCCGc     >  1:6698272/1‑101 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGc     <  2:3432192/101‑1 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGc     >  1:1017783/1‑101 (MQ=255)
                                                                                            aTAGCCCG.AACCCCGCCCCCCCGCACGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGTCCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGc     <  2:1233584/101‑1 (MQ=255)
                                                                                              aGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCg   >  2:4431900/1‑101 (MQ=255)
                                                                                              aGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCg   >  2:222832/1‑101 (MQ=255)
                                                                                                    |                                                                                                
AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCGG  >  NC_014034/2551210‑2551406

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG