Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,725,840 Δ1 bp 3.4% intergenic (‑112/+85) cysT ← / ← cysK1 sulfate ABC transporter permease CysT/cysteine synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,725,8400C→. 3.4% 27.0 0.0 ‑0.3353intergenic (‑112/+85)cysT/cysK1sulfate ABC transporter permease CysT/cysteine synthase
Reads supporting (aligned to +/- strand):  new base (8/4):  ref base (211/129):  total (220/133)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.33e-01

Only 100 of 425 total aligned reads displayed.

TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940
                                                                                                     |                                                                                                    
tccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.c                                                                                                      <  1:2178103/101‑1 (MQ=255)
tccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.c                                                                                                      <  1:5935939/101‑1 (MQ=255)
 ccttgcGTGCGCTTCGTCTTGTCGAAAATATCCACTGGGCCAGTCGAGATTGAAAGGGATAAATCCCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     <  2:2738041/101‑1 (MQ=255)
 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     >  2:5657804/1‑101 (MQ=255)
 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     >  2:6910089/1‑101 (MQ=255)
 ccttccGTGAGCTTCGTCATCTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     <  1:5623860/101‑1 (MQ=255)
 ccgtccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     <  2:3712279/98‑1 (MQ=255)
  cttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCCTTCTTGGTCGTGAATATAGAATTTTATT.ccc                                                                                                    >  1:3436446/1‑101 (MQ=255)
  cttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccc                                                                                                    >  1:4047159/1‑101 (MQ=255)
  cttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccc                                                                                                    <  1:5355419/101‑1 (MQ=255)
  cttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccc                                                                                                    >  1:476587/1‑101 (MQ=255)
    tccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccc                                                                                                  <  1:763460/101‑1 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 <  1:1475402/101‑1 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 <  2:2445594/101‑1 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 <  1:5038321/101‑1 (MQ=255)
      cGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccc                                                                                                <  1:6098151/101‑1 (MQ=255)
      cGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccc                                                                                                >  2:191052/1‑101 (MQ=255)
         gAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccccc                                                                                             <  2:6620512/101‑1 (MQ=255)
         gAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccccc                                                                                             >  2:1796036/1‑101 (MQ=255)
              tCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTcc                                                                                        >  2:5266609/1‑101 (MQ=255)
               cGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTccc                                                                                       >  1:4603073/1‑101 (MQ=255)
                gTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTcccc                                                                                      <  1:1896671/101‑1 (MQ=255)
                gTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTcccc                                                                                      >  2:6229492/1‑101 (MQ=255)
                gTCATTTCCAAAATATCCACGGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTccccc                                                                                     >  1:6383334/1‑101 (MQ=255)
                 tCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTccccc                                                                                     >  1:1126363/1‑101 (MQ=255)
                 gcATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTccccc                                                                                     <  2:2352158/100‑1 (MQ=255)
                  cATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCg                                                                                    >  1:6031066/1‑101 (MQ=255)
                  cATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCC.CTccccccg                                                                                   >  1:2826601/1‑99 (MQ=255)
                     ttCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCACGTGc                                                                                 <  1:4237888/101‑1 (MQ=255)
                          aaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                            >  2:617956/1‑101 (MQ=255)
                          aaaTATCCACTGTGGCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                            <  2:1039485/101‑1 (MQ=255)
                            atatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgcg                                                                          >  2:7114141/1‑101 (MQ=255)
                            atatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgcg                                                                          >  2:2577406/1‑101 (MQ=255)
                            atatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgcc                                                                          >  2:5620285/1‑100 (MQ=255)
                            atatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgcc                                                                          >  2:6067189/1‑100 (MQ=255)
                                  aCTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGCGCCAgcc                                                                    >  1:3934619/1‑99 (MQ=255)
                                    tgtgTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTCCCGGGGCGCCAGCCGa                                                                  >  1:270616/1‑101 (MQ=255)
                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaa                                                                 <  1:4330467/101‑1 (MQ=255)
                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaaa                                                               >  2:6322895/1‑101 (MQ=255)
                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaaa                                                               <  1:1554671/101‑1 (MQ=255)
                                        tCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAg                                                              >  1:1711684/1‑101 (MQ=255)
                                        tCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAg                                                              <  1:5266875/101‑1 (MQ=255)
                                        tCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAg                                                              >  1:2968490/1‑101 (MQ=255)
                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAgg                                                             >  2:5378936/1‑101 (MQ=255)
                                            gcGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGccc                                                          <  2:674990/100‑1 (MQ=255)
                                              gagaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccg                                                        >  1:3782607/1‑101 (MQ=255)
                                               agaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccgc                                                       <  1:6067501/101‑1 (MQ=255)
                                                gaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccgcc                                                      <  2:6447726/101‑1 (MQ=255)
                                                  ttGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCGAGACGAAAAGGCCCCCCCgg                                                    >  2:3285106/1‑101 (MQ=255)
                                                   tGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCggg                                                   >  1:315844/1‑101 (MQ=255)
                                                    gAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgggg                                                  >  1:1189033/1‑101 (MQ=255)
                                                    gAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCggggg                                                 >  2:1477434/1‑101 (MQ=255)
                                                     aaaGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCggggg                                                 >  1:6908998/1‑101 (MQ=255)
                                                     aaaGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGCGGCCAGCCGAAAAGGCCCCGCCggggg                                                 >  1:2290272/1‑101 (MQ=255)
                                                       aGGGATAAACCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTGCCCCGTGCCGGGGCGCCTGACGAAAAGGCCCCGCCGGggggc                                               <  2:3397473/101‑1 (MQ=255)
                                                        gggATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcg                                              >  2:2077345/1‑101 (MQ=255)
                                                         ggATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcgg                                             <  2:4153645/101‑1 (MQ=255)
                                                           aTAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGCCCCCGCCGGggggcgggc                                           >  2:865565/1‑100 (MQ=255)
                                                             aaaTCTTGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAGGGCGCGCCCGGGGGGCGGGGcc                                         >  1:7053371/1‑101 (MQ=255)
                                                             aaaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGcc                                         >  1:2501244/1‑101 (MQ=255)
                                                               aTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  1:6702111/101‑1 (MQ=255)
                                                                tctcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  1:622687/101‑1 (MQ=255)
                                                                acgcGCCATTCTTGGGCCTGAATATAAGATTTTTTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  2:3203561/98‑1 (MQ=255)
                                                                   cGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGaa                                   >  1:6485701/1‑101 (MQ=255)
                                                                     ccATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAg                                 <  1:4828618/101‑1 (MQ=255)
                                                                     ccATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAg                                 <  2:3594635/101‑1 (MQ=255)
                                                                      cATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGa                                >  1:4703555/1‑101 (MQ=255)
                                                                         tCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgc                             >  2:1699920/1‑101 (MQ=255)
                                                                         tCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  2:1238181/1‑101 (MQ=255)
                                                                          cTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  1:101440/1‑101 (MQ=255)
                                                                          cTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  1:4181968/1‑101 (MQ=255)
                                                                           ttGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgg                           >  1:4417166/1‑101 (MQ=255)
                                                                           ttGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACCCgg                           >  1:1766539/1‑101 (MQ=255)
                                                                             gggcgggAATATAGGATTTTACT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         <  2:4602834/95‑1 (MQ=255)
                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         >  2:1152493/1‑101 (MQ=255)
                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         >  2:386201/1‑101 (MQ=255)
                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         <  1:2426354/101‑1 (MQ=255)
                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         <  2:5525425/101‑1 (MQ=255)
                                                                              gTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGa                        >  2:4451048/1‑101 (MQ=255)
                                                                              gTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       >  1:4920630/1‑101 (MQ=255)
                                                                               tCGTTAAAATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       <  2:5086400/101‑1 (MQ=255)
                                                                                ggTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      <  2:2212663/100‑1 (MQ=255)
                                                                                cGTGAATATAGAATTTTATTCCCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       >  2:4715241/1‑101 (MQ=255)
                                                                                cGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      <  2:4947738/101‑1 (MQ=255)
                                                                                 gTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTGGAAAAACGCGGGGAAAc                     >  2:6250440/1‑101 (MQ=255)
                                                                                  tGAATATAGAATTTTATC.CCCCCCCCCCGCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAcc                    <  1:893536/101‑1 (MQ=255)
                                                                                   gAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   >  2:5947141/1‑101 (MQ=255)
                                                                                    aaTATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGa                  >  2:3938957/1‑101 (MQ=255)
                                                                                      tataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                >  1:1977429/1‑101 (MQ=255)
                                                                                        taGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAg              <  1:3210548/101‑1 (MQ=255)
                                                                                        taGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAg              <  1:2077425/101‑1 (MQ=255)
                                                                                          gAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCC.GGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGcc           <  2:4363811/101‑1 (MQ=255)
                                                                                             ttttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGGGGGGAAACCGACCAGGCCgg         >  2:7071554/1‑101 (MQ=255)
                                                                                             ttttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAACCCGATCAGACCgg         >  1:1261598/1‑101 (MQ=255)
                                                                                               ttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGCCGCGGGGAAACCGATCAGGCCgggg       >  1:3629758/1‑101 (MQ=255)
                                                                                               ttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCgggg       >  1:5535500/1‑101 (MQ=255)
                                                                                                tATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgc      <  1:617785/101‑1 (MQ=255)
                                                                                                 ttt.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgcg     <  2:2987951/100‑1 (MQ=255)
                                                                                                   t.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGcc   <  1:5808997/101‑1 (MQ=255)
                                                                                                     ccccccccccACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGCCGCGGGGAAACCGATCAGGCCGGGGCGCCg  >  2:2884456/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG