Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,687 Δ1 bp 100% intergenic (+12/‑171) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6870G→.100.0% 198.9 217intergenic (+12/‑171)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (22/137):  ref base (56/0):  total (80/137)
Fisher's exact test strand distribution p-value = 6.40e-34
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.94e-23

Only 100 of 278 total aligned reads displayed.

ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779
                                                                                                     |                                                                                             
aCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACg                                                                                               <  2:4541428/101‑1 (MQ=255)
aCCCGAAATTCATCGAGACCGAAACGATACTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACg                                                                                               <  1:4970704/101‑1 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                           <  2:4833662/101‑1 (MQ=255)
      aaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggac                                                                                         <  2:2231180/101‑3 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                        >  1:5699081/1‑98 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                        <  1:5882970/101‑4 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactg                                                                                       <  2:1723967/101‑5 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggaccg                                                                                       >  1:6433418/1‑97 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggggc                                                                                      >  1:2954017/1‑97 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgc                                                                                      <  1:2293058/101‑6 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcggggaccgc                                                                                      >  2:6596328/1‑96 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggggctttc                                                                                     >  1:4198855/1‑96 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgcc                                                                                     <  1:5574329/101‑7 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgcc                                                                                     <  2:6484108/101‑7 (MQ=255)
           aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggggggggcc                                                                                    >  1:5994036/1‑95 (MQ=255)
              gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggcgccgcc                                                                                  >  2:2644106/1‑93 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccccc                                                                             <  1:960601/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTccccc                                                                             <  1:298150/101‑1 (MQ=255)
                  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                            <  1:3415734/101‑1 (MQ=255)
                     aaaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CGGCCGTCCCCCGCAc                                                                         >  2:5755771/1‑101 (MQ=255)
                      aaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcc                                                                        <  2:5576289/101‑1 (MQ=255)
                      aaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcc                                                                        <  1:6692614/101‑1 (MQ=255)
                        cGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcccc                                                                      <  2:2560578/101‑1 (MQ=255)
                         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccccc                                                                     <  2:2091362/101‑1 (MQ=255)
                           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                                   <  2:4775466/101‑1 (MQ=255)
                            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGCCCGCCCCCCCCCccccccc                                                                   >  1:4733781/1‑99 (MQ=255)
                            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                                  <  1:7056566/101‑1 (MQ=255)
                              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCCccccccccgc                                                                >  1:3814016/1‑96 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCCcgcg                                                               >  2:5168873/1‑101 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGcgcg                                                               >  2:4118095/1‑101 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGGTCTATCGCAACCCGATCCGCTGAGGCCACGCT.ACGCGGGG.CGGCCGGCCCCCCCCccccccccccc                                                               >  2:3583098/1‑95 (MQ=255)
                                aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCCGTCTCCCCCCccccccccccg                                                               >  1:3185529/1‑95 (MQ=255)
                                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtt                                                           <  1:3927882/101‑1 (MQ=255)
                                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtt                                                           <  2:5555498/101‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCTGCCCGCCCCCCCCCcccccccccggcttt                                                           >  2:4996750/1‑91 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCGCGCGGAttt                                                          >  2:5305548/1‑101 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAttt                                                          <  2:6187731/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGTCGGC.TCCCCCGCCCCCCCCGCCCGTAtttt                                                         >  2:4700899/1‑101 (MQ=255)
                                      tCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCCCCCGCAttttt                                                        >  2:2952652/1‑100 (MQ=255)
                                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCCCCCCCCGCGCCCATTTTTcc                                                      >  1:2200030/1‑101 (MQ=255)
                                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCCCCCCCCCCGCGGCTTTTTcc                                                      >  1:1974453/1‑101 (MQ=255)
                                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCGTCCCCCGCCCCCCCCGCGCGGATTTTgtc                                                      >  2:4140415/1‑99 (MQ=255)
                                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGcc                                                      <  1:2623130/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCACCCCCCCCGCGGATTTTTccc                                                     >  2:1507020/1‑100 (MQ=255)
                                            aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAgg                                                  <  1:6933301/101‑1 (MQ=39)
                                              cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCGGCCCCCGCACCCCCCGCGCGGATTTTGCCCAAGaa                                                >  1:775885/1‑101 (MQ=255)
                                               ggCTCGGTGCTCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCTCCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  1:4553553/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGGCGTCCCCCGCCCCCCCCGCCCCGATTTTGCCCAAGaaa                                               >  2:2453006/1‑101 (MQ=255)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  1:6317794/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  1:6142986/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  2:3629450/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  2:3880645/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                               <  1:2935719/101‑1 (MQ=38)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCGCGCGTATTTTGCCAAGGAAAACggg                                          >  1:1031536/1‑101 (MQ=37)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCCCCCCCCGCGCGGATTTTGCCCAGGGAAACcgg                                          >  2:2895177/1‑101 (MQ=255)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCCGCGCGGATTTTGCCAAGGAAAACggg                                          >  2:5406943/1‑101 (MQ=37)
                                                       gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCCCACCCCCTGCGCGTATTTTGCCCAGGCAAACCGGGcc                                       >  2:2312785/1‑101 (MQ=255)
                                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.g                                     <  1:3620267/101‑1 (MQ=38)
                                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGGCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.g                                     <  1:6596668/101‑1 (MQ=38)
                                                         gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.Gt                                    <  2:4910978/101‑1 (MQ=38)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCACCCCCCGCGCGGATTTTTCCCAGGAAAAAGGGGGC.ggt                                   >  1:6818522/1‑99 (MQ=255)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.Gtt                                   <  1:1293228/101‑1 (MQ=38)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCGGCCCCCCCCCCCCCCCCCCCGCATTTTTCCCAAGAAAAAGGGGGC.Gctt                                  >  1:3705769/1‑101 (MQ=255)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.Gttt                                  <  2:3071050/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCGCGCGGATTTTGCCAAGGAAAAAGGGGCC.GGTTc                                 >  2:1386011/1‑101 (MQ=255)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCCTCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAAAGGGGGC.GTTTc                                 >  1:5527792/1‑101 (MQ=25)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACCGCCGTTCCCCGCACCCCCTGCGCGTATTTTTCCAAAGAAAACGGGGCC.GCTTc                                 >  2:815511/1‑101 (MQ=255)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCCCCCCCCGCGCGGATTTTGCCCAAGCAAACGGGGCC.Gctttcc                               >  2:1085245/1‑98 (MQ=255)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCAc                               <  1:4426602/101‑1 (MQ=38)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCAc                               <  2:3313779/101‑1 (MQ=38)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCtt                            <  1:4799694/101‑1 (MQ=38)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTg                           <  2:6107803/101‑1 (MQ=38)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTg                           <  1:3492871/101‑1 (MQ=38)
                                                                   cTATCGCAACCCGATCCGCTGAGGCGACGCT.ACGGGGGG.CTGCCGGCCCCCGCCCCCCCCCCGCGGATTTTGCCAAGGAAAACGGGGCC.GGTTCACCCTgg                          >  1:5244482/1‑101 (MQ=21)
                                                                   cTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCCCCCCCCGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCCACTTgg                          >  2:1189626/1‑101 (MQ=25)
                                                                   cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCCTCCCCCGCCCCCCCCGCGCGTCTTTTGCCAAGGAAAACGGGGCC.GTTTCCCCTTgc                          >  1:1659559/1‑100 (MQ=255)
                                                                    tATGGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCGCGCGTATTTTGCCAAGGAAAGCGGGGCC.GGTTCACCTTTGc                         >  1:5601172/1‑101 (MQ=25)
                                                                    tATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCGCGCGTATTTTTCCAAGGAAAACGGGGCC.GTTTCACCTTGGc                         >  1:2175911/1‑101 (MQ=37)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCGCACCCCCCCCGCGGATTTTTCCAAAGGAAACGGGGCC.GGTTTCCCCTGGcc                        >  2:2678564/1‑101 (MQ=255)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGCCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGcc                        <  2:580201/101‑1 (MQ=37)
                                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCg                       <  2:7043070/101‑1 (MQ=38)
                                                                       cGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGa                      <  2:3185416/101‑1 (MQ=38)
                                                                        gCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGaa                     <  1:5489061/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GCGCCCGCCCCCCCCCCCCCCGCGCGGGTTTTTCCCAAGAAAAAGGGGCCGGTTTCCCCTTGGCCAaaa                    >  1:2667515/1‑101 (MQ=255)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCCGCCCCCGCCCCCCCTCCGCGCAGTTTGCCAAGGAAAACGGGGGC.GTTTCACCCTGGCCGAAat                   >  2:1826676/1‑101 (MQ=255)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAat                   <  2:3768295/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAat                   <  1:2884888/101‑1 (MQ=38)
                                                                             ccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATc                <  1:6826348/101‑1 (MQ=38)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATcc               <  2:592625/101‑1 (MQ=38)
                                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATccc              <  1:4372573/101‑1 (MQ=38)
                                                                                aTCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCCCCCCCCGCCCCCCCCGCGCGGATTTTGCCCAGGAAAAAGGGGCC.GGTTCCCCTTTGGCGGAATATCCCg             >  1:1124927/1‑101 (MQ=255)
                                                                                  ccGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCggg           <  2:5674506/101‑1 (MQ=38)
                                                                                   cGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCgggg          <  2:916410/101‑1 (MQ=38)
                                                                                    gCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCggggg         <  2:1982710/101‑1 (MQ=38)
                                                                                     cTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCCCCCCCTGCGCGGATTTTGCCAAGGAAAACGGGGCC.GTTTCACCCTGGCCGAAATATCCCgggggg        >  1:635548/1‑101 (MQ=38)
                                                                                      tGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCggggggg       <  2:5974486/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GTGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCGGGGGGGa      <  1:6249199/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:2476528/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:4650800/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGCCGGCCCCCGCGCCCCCTGCGCGGATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGTCGAAATATCCCGGGGGGGAAttt  <  2:6868850/101‑1 (MQ=37)
                                                                                                     |                                                                                             
ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCC.GTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG