BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,439,846	Δ1 bp	100%	intergenic (‑149/+14)	RCAP_rcc03240 ← / ← mutB	ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,439,846	0	C→.	100.0%	17.1	205	intergenic (‑149/+14)	RCAP_rcc03240/mutB	ATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand): new base (197/0): ref base (0/8): total (197/8)
Fisher's exact test strand distribution p-value = 1.48e-14
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.91e-04

Only 100 of 290 total aligned reads displayed.


GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGACC  >  NC_014034/3439746‑3439944


gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cccc                                                                                                     <  1:882130/101‑1 (MQ=255)


gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cccc                                                                                                     <  1:4556567/101‑1 (MQ=255)


 gtgactttcGATGCCTCCGGCGGGGGTATTTTGGGCAAGATGAA.ACCCATCCCCTTCATCT.TT.CCCCCAAAATCCCGGGGGGCCCGGGGGGCAGCGc.cccc                                                                                                     >  1:2695008/9‑101 (MQ=25)


 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcg                                                                                                    <  2:1258793/101‑1 (MQ=255)


 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcg                                                                                                    <  1:1665065/101‑1 (MQ=255)


  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.ccccc                                                                                                    <  1:2135005/94‑2 (MQ=25)


  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.ccccc                                                                                                    <  2:5759785/94‑2 (MQ=25)


  cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcgc                                                                                                   >  2:4124060/1‑101 (MQ=255)


     ctttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                                                                                 <  1:2045940/97‑5 (MQ=25)


     ctttcGATGCCTCCGGCGGGGATATTNGNGNCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                                                                                 <  1:4585024/97‑5 (MQ=21)


     cGGCCGATGCCTCCGGCGGGGATATTTGGGGCCAGATAAAGGGGCTTTGGTTTTATCT.TT.TCCCCAATATCCCGGGGGTGG..GGGGGCCGAGG.CCCcgccgc                                                                                                >  2:1787442/1‑98 (MQ=37)


      ggCCGATGCCTCCGGCGGGGATATTTGGGGCAAGTTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCCcgccgcc                                                                                               >  1:4130178/1‑97 (MQ=255)


       ttcGATGCCTCCGGCGGGGATATTTTGGGCAAGATGAA.ACCCATCCCTTTCATCT.TT.CCCCCAATATCCCGGGGGGCC..CGGGGGGGAGCGCCCCCcg.cccc                                                                                             >  1:4343352/3‑99 (MQ=21)


       gCCGATGCCTCCGGCGGGGATATTTTGGGGAAAATAAAAGGGGTTTGCTTTTTTTT.TTCCCCCAAAAACCCCGGGGGGGG..GGGGGCGGAGc.cccccccgcc                                                                                               >  2:4715367/1‑95 (MQ=255)


       gCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCCcgccgcca                                                                                              >  1:3075811/1‑96 (MQ=255)


        ccGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCCcgccgcccc                                                                                             >  1:2932390/1‑95 (MQ=21)


        ccGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcgccgccac                                                                                             >  2:2957496/1‑95 (MQ=255)


           aTGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACggg                                                                                       >  1:387912/1‑100 (MQ=255)


               cTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTccc                                                                                   >  2:5256720/1‑99 (MQ=18)


                 cggggggggTTTTTTGGGGAAAGAAAAAGGGG.TTTTTTTTTTTCTCTT.CCCCAAA.ATCCCCGGGGGGG..GGGGGGGCAGC.CCCCCCG.CCGCCACGGTTCAcg                                                                                  <  2:1708078/96‑1 (MQ=255)


                 ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCAcgc                                                                                 >  2:1361743/1‑101 (MQ=255)


                   ggcggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGGTCAccccg                                                                               >  1:5523315/1‑101 (MQ=18)


                        ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGAcc                                                                          >  2:4834090/1‑101 (MQ=255)


                         gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCg                                                                         >  2:3332655/1‑101 (MQ=255)


                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGt                                                                        >  1:1243116/1‑101 (MQ=255)


                           tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGtc                                                                       >  2:7077351/1‑101 (MQ=255)


                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGtct                                                                      >  2:672834/1‑101 (MQ=255)


                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGtct                                                                      >  1:1142901/1‑101 (MQ=255)


                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGtct                                                                      >  2:5963222/1‑101 (MQ=255)


                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCg                                                                   >  2:2804644/1‑101 (MQ=255)


                                   gCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACTGTCTCCGAGGt                                                               >  2:1090177/1‑101 (MQ=21)


                                    cAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                                                              >  1:255660/1‑101 (MQ=255)


                                      aGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                                                            >  1:2785758/1‑101 (MQ=255)


                                      aGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                                                            >  1:6813122/1‑101 (MQ=255)


                                       gATAAGGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                                                           >  2:3515738/1‑101 (MQ=255)


                                       gATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                                                           >  2:1595707/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  2:1473301/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  2:2738773/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  1:4204189/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  1:1536312/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  1:4796971/1‑101 (MQ=255)


                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGccg                                                          >  1:5200695/1‑100 (MQ=25)


                                          aagggggTTTTTTTTTTTTCT.CTCCCCCAAATTCCCCGGGGGGGG..GGGGGGCG.GC.CCCCCCC.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          <  1:4455073/98‑1 (MQ=255)


                                           aaGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                                       >  2:1428373/1‑101 (MQ=255)


                                            aGGTGCTTTGCTTTTATCT.TT.CCCCAAATATCCGGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                                      >  1:5020564/1‑101 (MQ=37)


                                            aGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                                      >  1:1880328/1‑101 (MQ=255)


                                            aGGGGCTCTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                                      >  2:443185/1‑101 (MQ=255)


                                             ggggCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg                                                     >  2:2664079/1‑101 (MQ=255)


                                                tttttgttttTTTC..TT.CCCCAAAT.CGCGGGGGGGGG..GGGGGGGCAGC.CCCCCCC.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg                                                  <  2:759948/99‑1 (MQ=255)


                                                 ctttgctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                                 >  2:2499272/1‑101 (MQ=255)


                                                  tttgctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat                                                >  1:6999652/1‑101 (MQ=255)


                                                     gctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc                                             >  1:6395153/1‑101 (MQ=255)


                                                     gctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc                                             >  2:2584954/1‑101 (MQ=255)


                                                     gctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc                                             >  2:6271281/1‑101 (MQ=255)


                                                     gctttTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAGATc                                             >  2:6817560/1‑101 (MQ=255)


                                                     gctttTATCA.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc                                             >  1:1040084/1‑101 (MQ=255)


                                                        tttATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat                                          >  2:4859960/1‑101 (MQ=255)


                                                         ttATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  1:6064480/1‑101 (MQ=255)


                                                         ttATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  1:6492985/1‑101 (MQ=255)


                                                         ttATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  2:6141186/1‑101 (MQ=255)


                                                          tATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca                                        >  2:5084918/1‑101 (MQ=255)


                                                          tATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGCGCGCCGGCGCGACGATCTCcatca                                        >  2:5439454/1‑101 (MQ=25)


                                                           aTCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatcat                                       >  2:1978783/1‑101 (MQ=255)


                                                            tCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATg                                      >  1:5992503/1‑101 (MQ=255)


                                                             cT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                     >  1:4322786/1‑101 (MQ=255)


                                                                tt.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATc                                   >  1:5772234/1‑101 (MQ=255)


                                                                 t.CCCCAAATATCAAGGGGAAGA..GGGAGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGGCTCCGGGGGCGCGAGCGCGACGATATCCATCATGCTcc                                  >  2:2124633/1‑101 (MQ=255)


                                                                   ccccAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCg                                 >  1:2275411/1‑101 (MQ=255)


                                                                      cAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                              >  1:1410094/1‑101 (MQ=255)


                                                                        aaTATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCa                            >  2:698779/1‑101 (MQ=255)


                                                                         atatCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg                           >  2:3241983/1‑101 (MQ=255)


                                                                         agcgcgcGGGGGGGG..GGGGGCCCCCC.CCCCCCC.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                             <  1:3131751/95‑1 (MQ=255)


                                                                         agaggcggggggGGG..GGGGGCGCACC.CCCCCCC.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                             <  2:5891516/94‑1 (MQ=255)


                                                                           atCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCt                         >  1:7095977/1‑101 (MQ=255)


                                                                            tCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc                        >  1:2874813/1‑101 (MQ=255)


                                                                            tCCCGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc                        >  1:3258898/1‑101 (MQ=255)


                                                                             cccGGAGGGGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTCCACGCCGCGACCGTCTCCGGGGCAGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCg                       >  2:3777895/1‑101 (MQ=255)


                                                                              ccGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGa                      >  2:6483918/1‑101 (MQ=255)


                                                                               cGGGGGTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaa                     >  2:3646014/1‑101 (MQ=255)


                                                                                gggggTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa                    >  2:3899575/1‑101 (MQ=255)


                                                                                gggggTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa                    >  1:3355033/1‑101 (MQ=255)


                                                                                gggggTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa                    >  2:1385530/1‑101 (MQ=255)


                                                                                  gggTGC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATc                  >  1:2725473/1‑101 (MQ=255)


                                                                                      gC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTc              >  2:3468365/1‑101 (MQ=255)


                                                                                      gC..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTCCAGCTCGAACGCCTTc              >  2:1907646/1‑101 (MQ=255)


                                                                                       c..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg             >  1:676677/1‑101 (MQ=255)


                                                                                       c..GGGGGCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg             >  1:6628105/1‑101 (MQ=255)


                                                                                         ggggggtctcgC.CCGCCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCtt               <  2:5822108/91‑1 (MQ=255)


                                                                                          gggggCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCgg            >  2:231948/1‑101 (MQ=255)


                                                                                          gcgggcgacgC.CCCTGCGTCCGCCAGGGTTCACGCCGCGGCCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCtt               <  2:1654127/92‑1 (MQ=255)


                                                                                          gcgcggccccc.ccccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTc              <  2:4326764/91‑1 (MQ=255)


                                                                                            gggCAGAGC.CCC..CG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCg          >  1:5758847/1‑101 (MQ=255)


                                                                                                  ccc.ccccCCC.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtata      <  1:3647033/99‑1 (MQ=255)


                                                                                                  cagagcccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtat       >  2:1746010/6‑101 (MQ=255)


                                                                                                   agagcccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtata      >  1:2458741/5‑101 (MQ=255)


                                                                                                   agagcccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtata      >  2:4204764/5‑101 (MQ=255)


                                                                                                   agagcccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtata      >  1:3306345/5‑101 (MQ=255)


                                                                                                    gagcccCCG.CCGCCACGGTTCACCCCGCGACCGTCTCCGGGGCCGCCAGCGCGACGATATCCATCATGCTCTGGCTCTGCTTGCAATCCTTCGCCGTATAg     >  2:4482625/4‑101 (MQ=255)


                                                                                                     agcccCCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATACCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGa    >  1:6216315/3‑101 (MQ=255)


                                                                                                      gcccCCG.CCGCCCCGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCACGATCCGGTTCAGCTCGAAATCCTTCAGCGTATAGAc   >  1:6288448/2‑101 (MQ=255)


GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCG.CCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGACC  >  NC_014034/3439746‑3439944

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG