Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,372 Δ1 bp 100% intergenic (‑82/+48) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3720G→.100.0% 254.6 233intergenic (‑82/+48)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (70/94):  ref base (43/26):  total (113/120)
Fisher's exact test strand distribution p-value = 6.65e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.19e-01

Only 100 of 289 total aligned reads displayed.

GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461272‑3461462
                                                                                                    |                                                                                            
ggAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAaaggaag                                                                                              >  2:818414/1‑101 (MQ=255)
 gAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAaaggaagg                                                                                             >  1:5835784/1‑101 (MQ=255)
  gaTTTCAGATCTTGCATCGCAAGGCCCCCTATTCTCACCCGCAGCTTAGCAGGAGGCACGGGCGCGGCCAGACTCTGTTTGGCGCAGGGCCAAAGGAAggg                                                                                            <  1:1170932/100‑1 (MQ=255)
  aaTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg                                                                                            >  1:804277/1‑101 (MQ=255)
  aaTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg                                                                                            >  2:5843527/1‑101 (MQ=255)
   aTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggg                                                                                           >  2:2282317/1‑101 (MQ=255)
    tttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggggg                                                                                          >  2:6876937/1‑101 (MQ=255)
    tttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggggg                                                                                          >  1:4160710/1‑101 (MQ=255)
     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCCGCTTAGCAGGGTGCACGGGCGCGGCCAGACGCAGTTTGGCGCCGGGCCAAAGGAAggagg.g                                                                                        >  2:2848768/1‑101 (MQ=255)
     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggc                                                                                         <  2:3935303/101‑2 (MQ=255)
     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggc                                                                                         >  2:5161534/1‑100 (MQ=255)
      tCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcg                                                                                        >  2:2125295/1‑99 (MQ=255)
       cAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgc                                                                                       >  2:756821/1‑98 (MQ=255)
       cAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgc                                                                                       >  2:2665564/1‑98 (MQ=255)
        aGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgca                                                                                      <  2:2327105/101‑5 (MQ=255)
        aGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgca                                                                                      >  2:2618027/1‑97 (MQ=255)
        aGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgca                                                                                      >  1:4828452/1‑97 (MQ=255)
         gATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAgggggcgcag                                                                                     >  1:6902255/1‑96 (MQ=255)
          aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGc                                                                                    >  2:6702099/1‑101 (MQ=255)
          aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGc                                                                                    >  1:4765458/1‑101 (MQ=255)
           tCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcc                                                                                   <  2:781415/101‑1 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                  >  1:2361536/1‑100 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                  >  2:2910379/1‑100 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                  <  2:3198357/101‑2 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                  <  1:6198706/101‑2 (MQ=255)
             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 <  2:188801/101‑3 (MQ=255)
             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 >  2:5043340/1‑99 (MQ=255)
             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                                 >  2:5825920/1‑99 (MQ=255)
              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacc                                                                                >  1:3127714/1‑98 (MQ=255)
              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacc                                                                                >  2:1763409/1‑98 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTTGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               <  1:6366852/101‑5 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               >  2:4333917/1‑97 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               >  2:6072025/1‑97 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                               <  1:6723838/101‑5 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGCCGCcc                                                                               >  2:5765391/1‑101 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                              <  2:2060733/101‑6 (MQ=255)
                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                             >  2:5586517/1‑95 (MQ=255)
                    gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccg                                                                          >  1:1673351/1‑92 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  1:1396406/1‑91 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  2:2941067/1‑91 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  1:4359736/1‑91 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  1:4033322/1‑91 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  2:5286376/1‑91 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                         >  1:1565096/1‑91 (MQ=255)
                                       cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                 >  2:3447805/1‑101 (MQ=255)
                                        ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                <  2:5360239/101‑1 (MQ=255)
                                         cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  2:4028625/101‑1 (MQ=255)
                                         cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  1:1006535/101‑1 (MQ=255)
                                         cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               >  1:2950459/1‑101 (MQ=255)
                                          gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  1:3390608/101‑1 (MQ=255)
                                          gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGCGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  1:4521454/1‑101 (MQ=255)
                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                             <  2:4933588/101‑1 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  2:1623832/101‑1 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  1:3939182/101‑1 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  1:5681626/101‑1 (MQ=255)
                                              cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          <  2:7095653/101‑1 (MQ=255)
                                              cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          <  1:2536688/101‑1 (MQ=255)
                                               ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                         <  1:6049817/101‑1 (MQ=255)
                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                        >  1:3613019/1‑101 (MQ=255)
                                                  gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:540099/1‑101 (MQ=255)
                                                   cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  2:6113312/101‑1 (MQ=255)
                                                    aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGTGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:2071850/101‑1 (MQ=255)
                                                    aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  1:3516486/101‑1 (MQ=255)
                                                     ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGTAGGGCCAAAGGAA.GGGG.GC.GCAG...CTACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   <  2:3399755/101‑1 (MQ=255)
                                                      gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  <  1:5845263/101‑1 (MQ=255)
                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 >  1:5502945/1‑101 (MQ=255)
                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 >  2:2694967/1‑101 (MQ=255)
                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  1:6632138/101‑1 (MQ=255)
                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                >  1:5552397/1‑101 (MQ=255)
                                                           aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                             >  2:3588380/1‑101 (MQ=255)
                                                             gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgagatc                           >  1:1208393/1‑97 (MQ=255)
                                                             gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           >  2:4097841/1‑101 (MQ=255)
                                                              ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:2113717/101‑1 (MQ=255)
                                                               gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         >  2:2416352/1‑101 (MQ=255)
                                                               gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  2:3860381/101‑1 (MQ=255)
                                                                cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:4677862/101‑1 (MQ=255)
                                                                 gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       <  1:7121077/101‑1 (MQ=255)
                                                                 gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       >  1:758376/1‑101 (MQ=255)
                                                                  cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacggcgttct                      <  2:3772661/101‑8 (MQ=255)
                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:1505370/101‑1 (MQ=255)
                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     >  2:6270836/1‑101 (MQ=255)
                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:2682456/101‑1 (MQ=255)
                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:2125375/101‑1 (MQ=255)
                                                                     ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   <  1:3856747/101‑1 (MQ=255)
                                                                      cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  1:3553844/1‑101 (MQ=255)
                                                                        gACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGCTGACGTCAGAgg                >  2:1484072/1‑101 (MQ=255)
                                                                        gACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                <  1:2848877/101‑1 (MQ=255)
                                                                         aCGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc               <  2:6481990/101‑1 (MQ=255)
                                                                          cGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              >  1:995725/1‑101 (MQ=255)
                                                                             aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:5265569/101‑1 (MQ=255)
                                                                             aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:1609073/101‑1 (MQ=255)
                                                                               tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  1:2013702/101‑1 (MQ=255)
                                                                               tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         >  1:5341571/1‑101 (MQ=255)
                                                                                 tGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga       <  1:1913196/101‑1 (MQ=255)
                                                                                  ggCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      >  2:5890783/1‑101 (MQ=255)
                                                                                  ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:4219351/101‑1 (MQ=255)
                                                                                   gcgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     <  2:4642029/101‑1 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg    <  2:1150068/101‑1 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg    <  2:3612870/101‑1 (MQ=255)
                                                                                      cAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  2:5526474/1‑101 (MQ=255)
                                                                                                    |                                                                                            
GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461272‑3461462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG