Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,379 Δ1 bp 100% intergenic (‑89/+41) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3790A→.100.0% 313.8 232intergenic (‑89/+41)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (70/94):  ref base (42/26):  total (112/120)
Fisher's exact test strand distribution p-value = 9.45e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.71e-01

Only 100 of 236 total aligned reads displayed.

ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461282‑3461462
                                                                                                   |                                                                                    
aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGc                                                                                    >  1:653870/1‑101 (MQ=255)
aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGc                                                                                    >  1:4765458/1‑101 (MQ=255)
 tCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcc                                                                                   <  2:6015553/101‑1 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcca                                                                                  >  2:791956/1‑100 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcca                                                                                  <  2:1743969/101‑2 (MQ=255)
   tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  1:674834/1‑99 (MQ=255)
   tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  1:5528762/1‑99 (MQ=255)
   tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  2:69823/1‑99 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAG.GGGGGCGCAGCCgcc                                                                                >  2:5719136/1‑99 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  1:3860544/1‑98 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                <  2:4038575/101‑4 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  2:2724439/1‑98 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  2:4333917/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  2:6072025/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  1:1173602/1‑97 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccg                                                                              <  2:318453/101‑6 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccg                                                                              <  2:2060733/101‑6 (MQ=255)
       aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccgg                                                                             >  2:5586517/1‑95 (MQ=255)
        tCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggc                                                                            <  2:289227/101‑8 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccg                                                                          >  2:7105402/1‑92 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccg                                                                          <  1:791980/101‑10 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  1:4359736/1‑91 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  2:2941067/1‑91 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  2:5286376/1‑91 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  1:1565096/1‑91 (MQ=255)
                             cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                 >  2:3447805/1‑101 (MQ=255)
                              ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                <  2:5360239/101‑1 (MQ=255)
                               cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  1:1006535/101‑1 (MQ=255)
                                gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  2:3630195/1‑101 (MQ=255)
                                gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGCGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  1:4521454/1‑101 (MQ=255)
                                 cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                             <  2:4933588/101‑1 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           <  2:1623832/101‑1 (MQ=255)
                                    cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAATGGGGC.GCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGCCGTTCGATCa                                          >  2:5835327/1‑101 (MQ=255)
                                    cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          <  2:1785502/101‑1 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTTGATCAGt                                        <  2:674081/101‑1 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                        >  1:3613019/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:3375971/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:587662/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  1:352481/1‑101 (MQ=255)
                                        gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:929777/1‑101 (MQ=255)
                                        gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      <  1:1931925/101‑1 (MQ=255)
                                        gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:540099/1‑101 (MQ=255)
                                         cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  1:3120489/101‑1 (MQ=255)
                                          aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:5118608/101‑1 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   <  1:5843829/101‑1 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  2:6182803/1‑101 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  2:582465/1‑101 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  1:4820095/1‑101 (MQ=255)
                                            gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  >  2:2195819/1‑101 (MQ=255)
                                             aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  1:2182205/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:266706/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:778454/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:2071835/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                >  1:5552397/1‑101 (MQ=255)
                                              gatctgggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:1208345/96‑1 (MQ=255)
                                                cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              >  1:1459555/1‑101 (MQ=255)
                                                cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:5879924/101‑1 (MQ=255)
                                                 aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                             >  2:3588380/1‑101 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgagatc                           >  1:1208393/1‑97 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacgag                           >  1:3284036/1‑100 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:2616796/101‑1 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:1067194/101‑1 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           >  2:4097841/1‑101 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:6702441/101‑1 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  2:6271452/101‑1 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:2113717/101‑1 (MQ=255)
                                                     gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         >  2:2416352/1‑101 (MQ=255)
                                                     gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  2:3860381/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:1898078/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  1:6340557/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:4677862/101‑1 (MQ=255)
                                                       gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       <  1:7121077/101‑1 (MQ=255)
                                                        cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                      >  1:5653880/1‑101 (MQ=255)
                                                         ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:2682456/101‑1 (MQ=255)
                                                         ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     >  1:1762156/1‑101 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                    >  1:261360/1‑101 (MQ=255)
                                                           ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   >  1:1657789/1‑101 (MQ=255)
                                                            cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  1:3553844/1‑101 (MQ=255)
                                                            cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  2:3601845/1‑101 (MQ=255)
                                                            cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  <  1:2416446/101‑1 (MQ=255)
                                                             aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 >  1:4031206/1‑101 (MQ=255)
                                                              gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGCTGACGTCAGAgg                >  2:1484072/1‑101 (MQ=255)
                                                              gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                <  1:4108145/101‑1 (MQ=255)
                                                               aCGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc               <  2:5024700/101‑1 (MQ=255)
                                                                cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              <  1:1523597/101‑1 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  2:6556905/1‑101 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  1:2931461/1‑101 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  1:2791350/1‑101 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:1609073/101‑1 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:6152341/101‑1 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:5265569/101‑1 (MQ=255)
                                                                     tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  1:2013702/101‑1 (MQ=255)
                                                                     tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         >  1:5341571/1‑101 (MQ=255)
                                                                     tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  2:3892363/101‑1 (MQ=255)
                                                                      ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        >  2:3795512/1‑101 (MQ=255)
                                                                        ggCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:4219351/101‑1 (MQ=255)
                                                                         gcgcAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     >  2:3422676/1‑101 (MQ=255)
                                                                         gcgcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     <  2:4642029/101‑1 (MQ=255)
                                                                            cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  2:5526474/1‑101 (MQ=255)
                                                                            cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  1:3486827/1‑101 (MQ=255)
                                                                                                   |                                                                                    
ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461282‑3461462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG