Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,384 Δ1 bp 95.6% intergenic (‑94/+36) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3840A→.95.6% 20.6 ‑3.1 0.0173intergenic (‑94/+36)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (70/95):  ref base (6/1):  total (77/96)
Fisher's exact test strand distribution p-value = 4.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461287‑3461462
                                                                                                   |                                                                               
                       aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                  >  2:1148531/1‑101 (MQ=255)
                        cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                 >  2:3447805/1‑101 (MQ=255)
                         ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                <  2:5360239/101‑1 (MQ=255)
                          cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  2:4028625/101‑1 (MQ=255)
                          cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  1:1006535/101‑1 (MQ=255)
                           gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  2:4954240/1‑101 (MQ=255)
                           gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  1:6072337/101‑1 (MQ=255)
                           gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  1:3390608/101‑1 (MQ=255)
                           gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  2:3630195/1‑101 (MQ=255)
                           gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGCGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              >  1:4521454/1‑101 (MQ=255)
                            cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                             >  2:4379693/1‑101 (MQ=255)
                              gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           >  2:615219/1‑101 (MQ=255)
                               cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAATGGGGC.GCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGCCGTTCGATCa                                          >  2:5835327/1‑101 (MQ=255)
                               cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          <  1:2536688/101‑1 (MQ=255)
                                ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                         <  1:5365133/101‑1 (MQ=255)
                                ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                         <  2:2873463/101‑1 (MQ=255)
                                ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTAGATCAg                                         >  2:5786539/1‑101 (MQ=255)
                                 tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTTGATCAGt                                        <  2:674081/101‑1 (MQ=255)
                                 tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                        >  1:3613019/1‑101 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       <  2:3503568/101‑1 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       <  1:1058232/101‑1 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:587662/1‑101 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  1:165391/1‑101 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:7131720/1‑101 (MQ=255)
                                  aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  1:352481/1‑101 (MQ=255)
                                   gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  2:2062869/1‑101 (MQ=255)
                                   gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:929777/1‑101 (MQ=255)
                                    cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  1:3120489/101‑1 (MQ=255)
                                    cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                     <  1:5765693/101‑1 (MQ=255)
                                     aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGTGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:2071850/101‑1 (MQ=255)
                                     aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:5118608/101‑1 (MQ=255)
                                     aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  1:3516486/101‑1 (MQ=255)
                                     aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    >  1:1607054/1‑101 (MQ=255)
                                      ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGTAGGGCCAAAGGAA..GGGG.GC.GCAG...CTACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   <  2:3399755/101‑1 (MQ=255)
                                      ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  1:4820095/1‑101 (MQ=255)
                                      ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  2:6182803/1‑101 (MQ=255)
                                       gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  <  2:4654047/101‑1 (MQ=255)
                                       gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  >  2:4072786/1‑101 (MQ=255)
                                       gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  <  1:5845263/101‑1 (MQ=255)
                                        aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  1:6632138/101‑1 (MQ=255)
                                        aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  2:1732466/101‑1 (MQ=255)
                                        aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 >  2:2694967/1‑101 (MQ=255)
                                        aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  1:2182205/101‑1 (MQ=255)
                                         tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:778454/101‑1 (MQ=255)
                                         gatctgggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:1208345/96‑1 (MQ=255)
                                           cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              >  1:1459555/1‑101 (MQ=255)
                                           cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:5879924/101‑1 (MQ=255)
                                           cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:5455777/101‑1 (MQ=255)
                                            aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                             >  2:3364711/1‑101 (MQ=255)
                                              gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgagatc                           >  1:1208393/1‑97 (MQ=255)
                                              gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:2616796/101‑1 (MQ=255)
                                              gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:1852444/101‑1 (MQ=255)
                                               ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:6702441/101‑1 (MQ=255)
                                               ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:1957617/101‑1 (MQ=255)
                                               ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  2:6271452/101‑1 (MQ=255)
                                               ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:2113717/101‑1 (MQ=255)
                                                gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  2:3860381/101‑1 (MQ=255)
                                                gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         >  2:2416352/1‑101 (MQ=255)
                                                gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  1:2730339/101‑1 (MQ=255)
                                                 cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:674814/101‑1 (MQ=255)
                                                 cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:1898078/101‑1 (MQ=255)
                                                 cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  1:6340557/101‑1 (MQ=255)
                                                  gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       >  1:758376/1‑101 (MQ=255)
                                                  gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       <  1:7121077/101‑1 (MQ=255)
                                                  gcgGCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                       <  1:5624657/101‑1 (MQ=255)
                                                   cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacggcgttct                      <  2:3772661/101‑8 (MQ=255)
                                                   cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                      >  1:5653880/1‑101 (MQ=255)
                                                    ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:1505370/101‑1 (MQ=255)
                                                     gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                    >  1:1534021/1‑101 (MQ=255)
                                                      ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   >  1:1657789/1‑101 (MQ=255)
                                                      ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   <  1:3856747/101‑1 (MQ=255)
                                                       cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  2:3601845/1‑101 (MQ=255)
                                                       cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  <  2:5682493/101‑1 (MQ=255)
                                                        aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 >  1:263801/1‑101 (MQ=255)
                                                        aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 <  2:5835482/101‑1 (MQ=255)
                                                        aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 >  1:4031206/1‑101 (MQ=255)
                                                         gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                <  1:2848877/101‑1 (MQ=255)
                                                         gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                >  2:1524949/1‑101 (MQ=255)
                                                          aCGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc               <  2:5024700/101‑1 (MQ=255)
                                                           cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              >  1:995725/1‑101 (MQ=255)
                                                           cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              <  2:4732795/101‑1 (MQ=255)
                                                            gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  2:6556905/1‑101 (MQ=255)
                                                            gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  1:2931461/1‑101 (MQ=255)
                                                              aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:5532663/101‑1 (MQ=255)
                                                              aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:5265569/101‑1 (MQ=255)
                                                               gTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga          >  1:4319811/1‑101 (MQ=255)
                                                                tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         >  1:5341571/1‑101 (MQ=255)
                                                                tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  2:3892363/101‑1 (MQ=255)
                                                                 ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        <  1:2461764/101‑1 (MQ=255)
                                                                 ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        <  1:3594011/101‑1 (MQ=255)
                                                                 ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        <  2:1352180/101‑1 (MQ=255)
                                                                 ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        >  2:6017651/1‑101 (MQ=255)
                                                                 ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg        >  2:3795512/1‑101 (MQ=255)
                                                                  tGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga       <  1:1913196/101‑1 (MQ=255)
                                                                  tGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga       >  2:2299258/1‑101 (MQ=255)
                                                                   ggCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  1:2058574/101‑1 (MQ=255)
                                                                   ggCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:4219351/101‑1 (MQ=255)
                                                                    gcgcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     <  2:4642029/101‑1 (MQ=255)
                                                                      gcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc   >  1:4719619/1‑101 (MQ=255)
                                                                       cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  2:5526474/1‑101 (MQ=255)
                                                                                                   |                                                                               
TCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461287‑3461462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG