Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,943 Δ1 bp 5.3% coding (161/1467 nt) RCAP_rcc03392 → hemolysin D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,9430C→. 5.3% 24.0 ‑0.7 ‑0.4188coding (161/1467 nt)RCAP_rcc03392hemolysin D
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (110/68):  total (115/73)
Fisher's exact test strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.77e-01

Only 100 of 227 total aligned reads displayed.

GCAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGTG  >  NC_014034/3596843‑3597043
                                                                                                     |                                                                                                    
  aaTCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccc                                                                                                    >  2:6706490/1‑101 (MQ=255)
  aaTCCACAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccc                                                                                                    >  2:3522357/1‑101 (MQ=255)
    cccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccccc                                                                                                  <  1:1376811/100‑1 (MQ=255)
     ccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccc                                                                                                 <  1:4590254/101‑1 (MQ=255)
     ccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccc                                                                                                 >  1:4944387/1‑101 (MQ=255)
      cccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccccccc                                                                                                <  2:5106201/101‑1 (MQ=255)
      cccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccccccc                                                                                                <  1:640574/101‑1 (MQ=255)
       ccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccccc                                                                                               >  1:694663/1‑101 (MQ=255)
       ccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccccc                                                                                               <  2:1075382/101‑1 (MQ=255)
         caccGCAAACCCCCCGCCCCGCTGCGCCCAACCGCCTTGGCGGGGGGAGAGCCCGGCCTTCCCGCCCCGGCCCCCCGGCGCGGACCGGAAA.cccccccccc                                                                                             <  2:6908456/99‑1 (MQ=255)
             ccAACCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGcc                                                                                         <  2:3753147/100‑1 (MQ=255)
              cAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                        <  1:2979026/101‑1 (MQ=255)
              cAAGCCCCCCCCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                        <  2:3155523/101‑1 (MQ=255)
                aGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCCAg                                                                                      >  2:3831974/1‑101 (MQ=255)
                 gccccccgccccccTCGGCCCGCCCGCGTTGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAgg                                                                                     <  2:395107/101‑1 (MQ=255)
                  ccccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCCGCCGCGgc                                                                                    >  2:6542972/1‑101 (MQ=255)
                     cccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCCGCCGAGGcgcg                                                                                 >  2:6803733/1‑101 (MQ=255)
                     ccccccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGACCGAACCGGAAG.CCCCCCCCCCGGCCGAGGcgcg                                                                                 <  1:3716578/101‑1 (MQ=255)
                      ccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCgg                                                                                >  2:1386130/1‑101 (MQ=255)
                      ccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCgg                                                                                <  2:5451842/101‑1 (MQ=255)
                        gccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAt                                                                              <  1:5559613/101‑1 (MQ=255)
                        gccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGAtc                                                                             >  1:3074593/1‑101 (MQ=255)
                        gccccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAt                                                                              <  1:4848828/101‑1 (MQ=255)
                         ccccccCCGGCCCGGCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtc                                                                             <  1:7099936/101‑1 (MQ=255)
                          cccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtct                                                                            <  1:4105080/101‑1 (MQ=255)
                          cccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtct                                                                            >  1:2484994/1‑101 (MQ=255)
                          cccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtct                                                                            <  2:150950/101‑1 (MQ=255)
                                cGGCCCGCCCGCGTTCCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGcccc                                                                      <  2:5631978/101‑1 (MQ=255)
                                cGGCCCGACCGCGTTGCAGGCGGCAGAGGCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGcccc                                                                      <  2:1792173/101‑1 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGGGCGGATCTCGCCCCg                                                                     >  2:6187193/1‑101 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGGCCGGACCCCCCCCCg                                                                     >  1:592912/1‑101 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                     >  1:2629771/1‑101 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                     >  2:4501472/1‑101 (MQ=255)
                                 ggCCCGACCGCGTTGAGGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                     <  1:3639325/101‑1 (MQ=255)
                                    ccGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGAGCGGATCTCAGCCCgggg                                                                  >  2:4762561/1‑100 (MQ=255)
                                      gACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGGCCGGATCCCCCCCCCGGGgg                                                                >  1:2696975/1‑101 (MQ=255)
                                      gACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCCGCCGGGGCGCGGAGCTCGCCCCGGGTgg                                                                >  2:803534/1‑101 (MQ=255)
                                      gACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGgc                                                               >  1:6348710/1‑101 (MQ=255)
                                       aCCGCGTTGCAGGCGGCAGAGCCCGGCCTCCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgc                                                               <  1:1564008/101‑1 (MQ=255)
                                             ttGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgct                                                         <  1:4169831/101‑1 (MQ=255)
                                             ttGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgct                                                         >  1:4400846/1‑101 (MQ=255)
                                             ttGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCgctgct                                                         >  2:2720684/1‑101 (MQ=255)
                                              tGCAGGCGGCAGGGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCTGCCGAGGCGCGGATCTCGCCCCGGGGGGCGctgctg                                                        >  1:5055471/1‑101 (MQ=255)
                                              tGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGctgctg                                                        <  2:3450566/101‑1 (MQ=255)
                                               gcgggcggcAGGGCCCGGCCTCCCCGCCCCGCCCCGCCGGAGCAACCCGGAGC.CCCCCCCCCCGGCCGAGGCGCGGCTCTCGCCCCGGGTGGCGCTGCTGa                                                       <  2:3398869/98‑1 (MQ=255)
                                                 aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCCGGGGGCGCTGCTGATc                                                     >  2:2224237/1‑101 (MQ=255)
                                                     ggcAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCt                                                 >  2:3583985/1‑101 (MQ=255)
                                                       cAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGCGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTAt                                               <  1:3402856/101‑1 (MQ=255)
                                                       cAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTAt                                               >  2:7133842/1‑101 (MQ=255)
                                                       cAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCCGCCGAGGCGCGGATCTCGCCCCCGGGGGCGCTGCTGATCGGCTAt                                               >  2:6433888/1‑101 (MQ=255)
                                                        agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATg                                              >  1:1956711/1‑101 (MQ=255)
                                                        agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGGGCGGATCCGGCCCCGGGGGGGGCTGCTGATCGGCTAtt                                              >  1:990517/1‑100 (MQ=255)
                                                                gCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCtt                                      <  1:6581878/101‑1 (MQ=255)
                                                                 ccctCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGACCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTg                                     <  1:5233257/98‑1 (MQ=255)
                                                                 ccTCCCCCCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTg                                     <  1:1811485/101‑1 (MQ=255)
                                                                  cTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGc                                    <  2:432631/101‑1 (MQ=255)
                                                                  cTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCCCGCTTGc                                    >  1:3882336/1‑101 (MQ=255)
                                                                   ttCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCg                                   <  1:869582/101‑1 (MQ=255)
                                                                    tCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCgg                                  >  1:1759890/1‑101 (MQ=255)
                                                                     cccGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGt                                 >  2:6096363/1‑101 (MQ=255)
                                                                     cccGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGt                                 >  2:3221261/1‑101 (MQ=255)
                                                                       ccccccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTc                               <  1:3503913/99‑1 (MQ=255)
                                                                        gCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCt                              >  1:3759620/1‑101 (MQ=255)
                                                                        gCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCt                              <  1:4636024/101‑1 (MQ=255)
                                                                          cccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCGCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTgg                            <  1:6178175/101‑1 (MQ=255)
                                                                           ccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGGGCGGATCTCGCCCCGGGGGGCGCGGCTGACCGGCTATGGACCGCCTGCGGtgccggc                           >  1:2189025/1‑95 (MQ=255)
                                                                            cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                          <  1:5781906/101‑1 (MQ=255)
                                                                            cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                          >  1:4037401/1‑101 (MQ=255)
                                                                            cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCCCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                          >  1:4150936/1‑101 (MQ=255)
                                                                            cGGCCCACCGGCGAGAAGCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                          <  2:1733153/101‑1 (MQ=255)
                                                                             ggCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCg                         >  2:6850657/1‑101 (MQ=255)
                                                                              gCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGGGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCgg                        >  1:1382845/1‑101 (MQ=255)
                                                                               cccGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCggg                       <  2:3068127/101‑1 (MQ=255)
                                                                               cccGCCGGAGCCAACCGGGAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCggg                       <  2:3882173/101‑1 (MQ=255)
                                                                                ccgccgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCgggg                      <  2:1599191/101‑1 (MQ=255)
                                                                                  gccgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGc                    <  1:3322664/101‑1 (MQ=255)
                                                                                  gccgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGc                    >  1:3476319/1‑101 (MQ=255)
                                                                                   ccgGGGCGAACCGGGAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCt                   <  1:5332551/101‑1 (MQ=255)
                                                                                    cgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                  >  2:90069/1‑101 (MQ=255)
                                                                                    cgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                  <  2:2484900/101‑1 (MQ=255)
                                                                                     ggAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTc                 >  2:4267135/1‑101 (MQ=255)
                                                                                     ggAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTc                 >  2:4812228/1‑101 (MQ=255)
                                                                                      gAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCg                >  1:4716908/1‑101 (MQ=255)
                                                                                      gAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCg                >  1:329876/1‑101 (MQ=255)
                                                                                       ggCGCACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCgg               <  2:4146962/100‑1 (MQ=255)
                                                                                       aGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTCTGTCCCGCTTCCGGTTCTGGGCGGGGGCTTCgg               >  1:1538264/1‑101 (MQ=255)
                                                                                         cGAACCGGAAGCCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCggg              >  1:2111279/1‑101 (MQ=255)
                                                                                          gAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCt            <  2:2928904/101‑1 (MQ=255)
                                                                                           aaCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt           <  1:53810/101‑1 (MQ=255)
                                                                                           aaCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt           >  1:1111508/1‑101 (MQ=255)
                                                                                            aCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt          <  1:683395/101‑1 (MQ=255)
                                                                                            aCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt          <  2:1230857/101‑1 (MQ=255)
                                                                                             ccGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTg         >  2:5062853/1‑101 (MQ=255)
                                                                                               ggAAC.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGt       <  1:4772395/101‑1 (MQ=255)
                                                                                                gAAG.CCCCCCCCCCGGCCGAGGGGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGGTTCGGGCTTTGGTc      >  2:4658850/1‑101 (MQ=255)
                                                                                                gAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc      >  2:5723366/1‑101 (MQ=255)
                                                                                                gAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc      >  2:5222908/1‑101 (MQ=255)
                                                                                                 aaG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGGTTGGGGCTTGggcgg     >  1:2434300/1‑98 (MQ=255)
                                                                                                 aaG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCg     >  1:1451024/1‑101 (MQ=255)
                                                                                                  aG.CCCCCCCCCCGGCCGGGGGGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGGTTCGGGCTTTGGGCgg    >  2:492781/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGTG  >  NC_014034/3596843‑3597043

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG