Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 3.1% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 3.1% 15.7 ‑0.8 ‑0.1255intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (159/88):  total (163/92)
Fisher's exact test strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.34e-01

Only 100 of 360 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902
                                                                                                     |                                                                                                     
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                      <  1:4004940/101‑1 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                      >  2:3253449/1‑101 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                    <  1:1779147/101‑1 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                    <  1:2614616/101‑1 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                    >  2:6544427/1‑101 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccc                                                                                                <  1:4399011/101‑1 (MQ=255)
         cTCCAGTTTCTTTGTGTGAACCGGGGCTTTCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccc                                                                                              <  2:1293182/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccc                                                                                              <  2:4162261/101‑1 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccccccc                                                                                             >  2:4422862/1‑100 (MQ=255)
           ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTT.GCCCGCCCCCgg                                                                                            >  2:3337483/1‑100 (MQ=255)
           ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccccc                                                                                            >  1:3415155/1‑99 (MQ=255)
           caaGTTGCTGTAGCTGAACCGGGTCTTACGCCTGTGGCTGGGGTGGGGCAATAACCCGGCCGGGCTTGGGAAGGCCCTGCGGCACCCTG.CCCCCCCCCCGa                                                                                            <  2:2481910/99‑1 (MQ=255)
            cAGTTTCTGTAGGGAAACCGGGGCTTCCCCCTGTGGCGGCGGGGGGGCAATGCCCCGCACGGGCTGGTGAAGGCGCTGCGGCATCCTC.CCCCCCCCCCGA.c                                                                                          <  2:1236741/101‑1 (MQ=255)
             aGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.Cg                                                                                         <  1:2940072/101‑1 (MQ=255)
             aGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCGG.CCCCCCCCCCCA.Cg                                                                                         >  2:6677782/1‑101 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGa                                                                                      <  2:2956802/101‑1 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGggg                                                                                    >  2:5265862/1‑101 (MQ=255)
                   tGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCA.CCGGaggg                                                                                   >  1:6125771/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCC.CGGGggggc                                                                                  >  2:2355811/1‑101 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGggggcg                                                                                 >  2:4097850/1‑100 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGagggca                                                                                 <  1:2866394/101‑1 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGA.CGGGagggcag                                                                                >  2:5031877/1‑101 (MQ=255)
                         tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGggggcagggc                                                                             >  1:3186399/1‑101 (MQ=255)
                            aCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTgg                                                                          >  1:3760390/1‑101 (MQ=255)
                              cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTgggt                                                                        >  2:6142337/1‑99 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGGTGGGGCATCCTG.CCCCCCCCCGGC.CGGGCGGGACGGGCGggggt                                                                       >  2:2284904/1‑98 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                       <  1:6352549/101‑1 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                       <  1:6624588/101‑1 (MQ=255)
                               ggggCTTACGCCTGGGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                       <  1:1904407/101‑1 (MQ=255)
                                   cTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGGGGGCAGGGCTGGGTTCggg                                                                   >  1:2510826/1‑101 (MQ=255)
                                   cTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCACCGGGGGGGCGGGGCGGGTTTGgg                                                                    >  2:6952412/1‑101 (MQ=255)
                                    ttACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGTTTCGggc                                                                  >  2:5532322/1‑101 (MQ=255)
                                    ttACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGGGGGCAGGGCTGGGTTCgggg                                                                  >  1:528394/1‑100 (MQ=255)
                                     tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.AGGGAGGGGAGGGCTGGGTTCggggg                                                                 >  2:3990585/1‑99 (MQ=255)
                                      tcGCCTGTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGggcgg                                                                <  2:2857608/100‑1 (MQ=255)
                                       cGCCTGGGGCGGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGggcggc                                                               <  1:1029183/101‑1 (MQ=255)
                                        gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGAGGGCAGGGCTGGTTTCGGGGGGCa                                                              >  2:5347112/1‑101 (MQ=255)
                                        gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                              <  2:1683419/101‑1 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGgc                                                          >  2:4667358/1‑101 (MQ=255)
                                             tgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgcg                                                         >  2:1870609/1‑101 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGGTTCGGGCGGCAAGGCGcc                                                       >  2:3478816/1‑101 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                       >  1:1651249/1‑101 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                       <  2:4838881/101‑1 (MQ=255)
                                                cTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                      >  1:5995302/1‑101 (MQ=255)
                                                   cGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                   >  2:2074630/1‑101 (MQ=255)
                                                   cGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                   >  1:4225502/1‑101 (MQ=255)
                                                    ggTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcgg                                                  >  1:6876009/1‑101 (MQ=255)
                                                       ggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgc                                               >  1:1225948/1‑101 (MQ=255)
                                                       ggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCACCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGAAAGGAGCCcgcgggcc                                               >  1:5606519/1‑98 (MQ=255)
                                                        gggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcccggcgc                                               >  1:988142/1‑101 (MQ=255)
                                                         ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                             >  2:5432664/1‑101 (MQ=255)
                                                         ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                             >  2:6212049/1‑101 (MQ=255)
                                                          gCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCgcggcgcggc                                            <  1:1979128/101‑1 (MQ=255)
                                                           cAATGCCCCGCACGGGGTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           <  2:4908992/101‑1 (MQ=255)
                                                           cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGGGGcc                                           >  1:4361927/1‑101 (MQ=255)
                                                            aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGGAAGGCGCCCGCGGCGCGGCCg                                          >  2:1206575/1‑101 (MQ=255)
                                                            aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCg                                          >  1:5870164/1‑101 (MQ=255)
                                                            aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCg                                          >  1:6421288/1‑101 (MQ=255)
                                                              tGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTg                                        >  2:1540334/1‑101 (MQ=255)
                                                                aCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcc                                      <  2:3598373/101‑1 (MQ=255)
                                                                 cccggccGGGTTTGGGAAGGCCCTGCGGCACCCTT.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     <  1:3378706/95‑1 (MQ=255)
                                                                 cccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     <  1:1542794/101‑1 (MQ=255)
                                                                 cccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     >  1:4515771/1‑101 (MQ=255)
                                                                  ccgctggggCTGGGGAAGGCGGTGCGGCACCCGG.CCCCGCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTg                                    <  2:5803611/95‑1 (MQ=255)
                                                                  ccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTTGGGCGGCAAGGCGCCCGCGGCGCGGCCGCGCCTg                                    >  2:4725179/1‑101 (MQ=255)
                                                                   cGCAGGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGc                                   <  2:978887/101‑1 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGcc                                  >  1:4969987/1‑100 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCA.CGGGGGGGCCGGGCTGGTTTTGGGCGGGAAGGGCCCCCCGGGGGGGCCGGGCCCGCt                                  >  1:2804446/1‑101 (MQ=255)
                                                                    ccACGGGCTTGTTAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCt                                  <  1:3269244/100‑1 (MQ=255)
                                                                     cgtgggCTGGGGAAGGCGCTGCGCCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCTGCCGTGCCTGCtt                                 <  1:1392365/98‑1 (MQ=255)
                                                                     cccGGGCTTGGGAAGGCGCCGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                 <  1:5035544/99‑1 (MQ=255)
                                                                     cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                 <  1:76640/101‑1 (MQ=255)
                                                                      cggggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  2:6850598/99‑1 (MQ=255)
                                                                      aCGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  2:5140764/101‑1 (MQ=255)
                                                                        gggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCCCCGGGGCCGCCGGGCCTggctaac                              >  2:5195176/1‑95 (MQ=255)
                                                                         gggttGTGAAGGCGCTGCGGCATCCGG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGc                             <  1:1002783/98‑1 (MQ=255)
                                                                           cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCt                           >  2:1398514/1‑101 (MQ=255)
                                                                           cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCt                           >  1:2005058/1‑101 (MQ=255)
                                                                            ttGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTg                          >  1:3053045/1‑101 (MQ=255)
                                                                             tgtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                         >  2:5686486/1‑101 (MQ=255)
                                                                              gtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        >  1:4895833/1‑101 (MQ=255)
                                                                              gtcaaGGCGCTGAGGCATCGTC.CCCCCCCCCCGA.CCGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGTCCTTGCCTGCTTACGCCTGCa                        <  2:4486875/98‑1 (MQ=255)
                                                                               tgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTTGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                       >  1:2168397/1‑101 (MQ=255)
                                                                                gAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGTGCCTGCTTACGCCTGGAgg                      >  1:4134869/1‑100 (MQ=255)
                                                                                    gctcggCGCCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                  <  1:2116221/96‑1 (MQ=255)
                                                                                    gcgcTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGGCGTGCCTGCTTACGCCTGCCGCgggc                  >  2:754053/1‑99 (MQ=255)
                                                                                         gCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGGcc             >  1:2324489/1‑101 (MQ=255)
                                                                                         gCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGAcc             <  1:6209426/101‑1 (MQ=255)
                                                                                           ggCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGACCgg           >  1:4746484/1‑100 (MQ=255)
                                                                                            gCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgag          <  1:924990/101‑1 (MQ=255)
                                                                                             cctCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgaga         <  1:6771098/99‑1 (MQ=255)
                                                                                             cATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGCCCgaga         >  2:2770500/1‑101 (MQ=255)
                                                                                             cATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgaga         >  2:2934370/1‑101 (MQ=255)
                                                                                              aTCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt        <  1:5846240/101‑1 (MQ=255)
                                                                                              aTCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt        <  2:1993506/101‑1 (MQ=255)
                                                                                                 cTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     <  2:1109022/101‑1 (MQ=255)
                                                                                                  tG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttttg    >  2:6464461/1‑99 (MQ=255)
                                                                                                   g.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGGCGGACCGAGATTTccc   >  2:70333/1‑99 (MQ=255)
                                                                                                   g.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGGTTTCGc   >  2:601846/1‑101 (MQ=255)
                                                                                                     ccccccccccGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGcc  <  1:520110/101‑1 (MQ=255)
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CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG