Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 904,926 Δ1 bp 98.4% intergenic (+30/‑166) RCAP_rcc00821 → / → murB hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034904,9260T→.98.4% 3.7 0.0 ‑0.5206intergenic (+30/‑166)RCAP_rcc00821/murBhypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (100/102):  ref base (1/2):  total (102/104)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.28e-01

Only 100 of 242 total aligned reads displayed.

TGGCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGC  >  NC_014034/904826‑905020
                                                                                                    |                                                                                                  
tGGCAGGCGGGCGGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCctct                                                                                                    <  1:5528384/101‑1 (MQ=255)
 ggCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGGCGCCCCCCTCtt                                                                                                   >  1:1780236/1‑101 (MQ=255)
 ggCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtt                                                                                                   <  1:3899171/101‑1 (MQ=255)
 ggCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtt                                                                                                   >  1:502348/1‑101 (MQ=255)
 ggCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtt                                                                                                   <  1:4585852/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                 <  2:637877/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                 <  1:4066512/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                 <  1:4775459/101‑1 (MQ=255)
    aGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCTGCCCCCCCCttttg                                                                                                >  1:4173271/1‑100 (MQ=255)
    aGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttg                                                                                                >  2:240362/1‑100 (MQ=255)
    aGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCCATGCACCGGCGCGACCGAGGGCGGGGCAGGGGGGCGCCGGCCCCCTCttttt                                                                                                >  2:4731118/1‑101 (MQ=255)
        gggCTGAGCGTGCTGCGCTGGCCGGTGCGGGGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGGTGGCCCCCTC.TTTTG.G.CT..g                                                                                       >  1:3993445/1‑101 (MQ=255)
        gggCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..g                                                                                       >  1:4154306/1‑101 (MQ=255)
          gCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcg                                                                                     <  2:5854408/101‑2 (MQ=255)
          gCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcg                                                                                     <  2:3237270/101‑2 (MQ=255)
             gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccc                                                                                  >  1:6669703/1‑97 (MQ=255)
             gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                  <  1:5545952/101‑5 (MQ=255)
             gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCC.CCTTTTG.G.CT..Gcgcca                                                                                  >  2:4034612/1‑97 (MQ=38)
               gCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaa                                                                                <  2:7027206/101‑7 (MQ=255)
                 gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                              >  1:4391708/1‑93 (MQ=37)
                 gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                              <  2:1948250/101‑9 (MQ=37)
                 gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                              >  1:100660/1‑93 (MQ=37)
                 gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                              >  1:2979184/1‑93 (MQ=37)
                 gTGCTGCGCTGGACGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGGTGGCCCCCTC.TTTTG.G.CT..Gcgcccaaat                                                                              >  1:311747/1‑93 (MQ=255)
                  tgctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaatt                                                                             >  2:4602732/1‑92 (MQ=25)
                  tgctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaatt                                                                             <  1:280621/101‑10 (MQ=25)
                   gctgcGCTGGCCGTTGCGGCGGCTCTTTGCCGCGATGCACCGGGGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTc                                                                              <  2:6811338/101‑1 (MQ=21)
                   gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTc                                                                              <  2:2426502/101‑1 (MQ=25)
                    ctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCa                                                                             <  2:6474232/101‑1 (MQ=25)
                     tgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCAc                                                                            <  2:6883218/101‑1 (MQ=25)
                          tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCAcccccc                                                                       <  2:5680620/101‑1 (MQ=21)
                           ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCg                                                                      >  1:5965542/1‑101 (MQ=21)
                              cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCg                                                                   >  1:730512/1‑101 (MQ=21)
                               ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTTACCCCCCCGcct                                                                  >  2:577040/1‑99 (MQ=21)
                               ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGt                                                                  <  1:7026494/101‑1 (MQ=21)
                               ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCCGccg                                                                  >  2:3123736/1‑99 (MQ=21)
                                gTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTa                                                                 >  1:1085318/1‑101 (MQ=21)
                                 tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTAt                                                                <  2:6223749/101‑1 (MQ=21)
                                  gcggcgGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCGGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTAtt                                                               >  2:1734667/1‑101 (MQ=21)
                                  gcggcgGCTCGTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTAtt                                                               <  1:5332420/101‑1 (MQ=21)
                                   ctgcgtcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTAttt                                                              <  2:3566119/99‑1 (MQ=18)
                                    ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTGG.NTGC..GCCAAAATTCAACCCCCGGCGTATTTg                                                             >  1:1248329/1‑101 (MQ=21)
                                    ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTg                                                             <  2:4571691/101‑1 (MQ=21)
                                    ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTg                                                             >  2:6569537/1‑101 (MQ=21)
                                     gcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGc                                                            >  2:683868/1‑101 (MQ=21)
                                      cggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGGCTTTGCa                                                           >  1:2575927/1‑101 (MQ=18)
                                       ggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGTGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCAc                                                          <  1:1601234/101‑1 (MQ=21)
                                       ggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCAc                                                          >  2:4083729/1‑101 (MQ=21)
                                        gcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCAcc                                                         >  1:6390667/1‑101 (MQ=21)
                                          tctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCCAAATTCACCCCCCGGCGGATTTGCACCAg                                                       >  2:49534/1‑101 (MQ=21)
                                           ctctGCCGCGATGCTCGGGCGCGGCTGAGGGCGGGGCAGGGGCGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAgg                                                      <  2:5619983/98‑1 (MQ=17)
                                           ctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCNAAATTCACCCCCCGGCGTATTTGCACCAgg                                                      >  1:2933035/1‑101 (MQ=21)
                                           ctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAgg                                                      <  2:3813676/101‑1 (MQ=21)
                                             ttGCCGCGATGCACCGGCACGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCTCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGaa                                                    <  2:1240396/101‑1 (MQ=21)
                                             gtGCCGCGATGCCCCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGaa                                                    <  1:3580670/100‑1 (MQ=18)
                                              tGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGaaa                                                   >  2:290057/1‑101 (MQ=21)
                                                ccGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGaaaaa                                                 >  2:5452588/1‑101 (MQ=21)
                                                  gcgATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGc                                               <  2:4497394/101‑1 (MQ=21)
                                                     aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCGAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                            <  1:2712113/101‑1 (MQ=21)
                                                       gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                          <  1:3912918/101‑1 (MQ=21)
                                                          ccGGCGCGACGGAGGGCGGGGCCGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtt                                       <  2:2753203/101‑1 (MQ=18)
                                                           cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                      <  1:6220331/101‑1 (MQ=21)
                                                            ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                     >  2:1335760/1‑101 (MQ=21)
                                                             gggcgACTGAGGGCGGGGCAGGGGGGCGCTGCCTCCCTC.TTTTG.G.CTGCGCCAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttttt                                    <  1:113890/99‑1 (MQ=25)
                                                               gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTcc                                  <  1:6809210/101‑1 (MQ=21)
                                                                 gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                                >  1:6634656/1‑101 (MQ=21)
                                                                   ctggtggCGGGGGCTGGGGGCGCTTCCCCCNTC.TTTTG.G.CTGCGCCAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                              <  1:2136307/96‑1 (MQ=21)
                                                                    tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             >  1:1543750/1‑101 (MQ=21)
                                                                    tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             >  1:334076/1‑101 (MQ=21)
                                                                    tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             >  1:6585851/1‑101 (MQ=21)
                                                                    tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             >  2:2222501/1‑101 (MQ=21)
                                                                      aGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGa                           >  1:865133/1‑101 (MQ=21)
                                                                       gggCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCTCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGaa                          <  1:1871954/101‑1 (MQ=21)
                                                                       gggCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGaa                          >  2:4288806/1‑101 (MQ=21)
                                                                         gCGGGGCCGGGGGGCGCTGCCCCCCTCTTTTAGCT.GC..GCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAAt                        <  2:6140629/101‑1 (MQ=21)
                                                                         gCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAAt                        <  2:5370921/101‑1 (MQ=21)
                                                                           ggggCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATAc                      <  1:4340803/101‑1 (MQ=21)
                                                                             ggCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGc                    >  2:398822/1‑101 (MQ=21)
                                                                              gCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                   <  1:682997/101‑1 (MQ=21)
                                                                              gCAGGGGGGCGCCGCCCCCCCCTTTTGG.GTGC..GCCAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                   <  2:6051194/101‑1 (MQ=37)
                                                                               cAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCg                  >  2:4931007/1‑101 (MQ=21)
                                                                                aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                 >  1:149732/1‑101 (MQ=25)
                                                                                aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                 >  2:5356451/1‑101 (MQ=25)
                                                                                 ggggggCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCggg                >  2:1050272/1‑101 (MQ=25)
                                                                                  gggggCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCGCGGCGTATGTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCGgggg               <  2:2591441/101‑1 (MQ=21)
                                                                                  gggggCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg               >  1:5686377/1‑101 (MQ=25)
                                                                                    gggCGCTGCCCCCCTC.GTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg             <  2:484087/101‑1 (MQ=25)
                                                                                     ggCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa            >  1:4749875/1‑101 (MQ=25)
                                                                                     ggCGCTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa            <  1:1403035/101‑1 (MQ=25)
                                                                                      gcgcTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAg           <  1:5356723/101‑1 (MQ=25)
                                                                                         cTGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        >  2:4248228/1‑101 (MQ=37)
                                                                                          tGCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg       <  1:1665263/101‑1 (MQ=37)
                                                                                           gCCCCCCTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGc      <  2:4754657/101‑1 (MQ=37)
                                                                                            ccccccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt     <  2:1085283/101‑1 (MQ=37)
                                                                                            ccccccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt     <  2:1628784/101‑1 (MQ=37)
                                                                                             cccccTC.TTTTG.G.CTGCGCCAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt    <  1:3416459/101‑1 (MQ=25)
                                                                                               cccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTgt  >  1:3274875/1‑100 (MQ=25)
                                                                                               cccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc  >  1:5999311/1‑101 (MQ=37)
                                                                                               cccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc  <  2:2145426/101‑1 (MQ=37)
                                                                                               cccTC.TTTTG.G.CTGCGCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc  <  2:6585511/101‑1 (MQ=37)
                                                                                                    |                                                                                                  
TGGCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGC  >  NC_014034/904826‑905020

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG