Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,003,050 +G 1.4% coding (356/738 nt) pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,003,0501.→G 1.4% 4.0 ‑3.6 ‑0.2284coding (356/738 nt)pssACDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (113/167):  total (117/167)
Fisher's exact test strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.76e-01

Only 100 of 323 total aligned reads displayed.

GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150
                                                                                                     |                                                                                                     
gTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCt                                                                                                        <  2:1465540/101‑1 (MQ=255)
gTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCt                                                                                                        >  1:3815419/1‑101 (MQ=255)
  cgcgCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.gg                                                                                                     <  1:6130552/101‑1 (MQ=255)
  cgcgCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.gg                                                                                                     <  1:3708938/101‑1 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggc                                                                                                  >  2:6683914/1‑98 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.gggcg                                                                                                  >  1:6824240/1‑98 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGccc                                                                                                  <  2:5839865/101‑1 (MQ=255)
       ccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggggg                                                                                                >  1:3216243/1‑96 (MQ=255)
        cGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcggg                                                                                               >  2:898628/1‑95 (MQ=255)
        cGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCggg                                                                                               >  1:6417961/1‑100 (MQ=255)
         ggggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGtt                                                                                              <  2:2580063/101‑1 (MQ=255)
          gggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGCCCgggg                                                                                              >  1:4935416/1‑99 (MQ=255)
          gggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcgggtc                                                                                             >  1:673612/1‑93 (MQ=255)
          gggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTc                                                                                             <  2:1966042/101‑1 (MQ=255)
           ggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCa                                                                                            <  2:132903/101‑1 (MQ=255)
           ggTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCa                                                                                            <  2:4407073/101‑1 (MQ=255)
            gTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCaa                                                                                           <  2:3947438/101‑1 (MQ=255)
            gTGCTCGTCTATCGCTTCGAGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcggggtga                                                                                           >  2:5177532/1‑91 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCTGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAAc                                                                                          <  1:5002617/101‑1 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCggggcgac                                                                                          >  2:5968786/1‑95 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGGCAAc                                                                                          >  1:5656170/1‑101 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAAc                                                                                          <  1:880982/101‑1 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAAc                                                                                          <  2:3800941/101‑1 (MQ=255)
                       tCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCggg                                                                                <  2:3000962/101‑1 (MQ=255)
                        cGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGGCGGGTCAACGCGGcccccgg                                                                               >  1:2889157/1‑97 (MQ=255)
                         gCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGc                                                                              <  2:4041526/101‑1 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGGCCGGTCTCCGCGACCCCGGGCg                                                                             >  2:4467672/1‑101 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCCCGCGGCCCCGGGCg                                                                             >  1:328872/1‑101 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCg                                                                             <  2:1679688/101‑1 (MQ=255)
                           ttagcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCga                                                                            <  2:7076133/98‑1 (MQ=255)
                             cgcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGGCGGGTCAACCCGACCCcgggggggg                                                                          >  1:4224640/1‑96 (MQ=255)
                             cgcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCgagg                                                                          <  2:5328625/101‑1 (MQ=255)
                                gATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGGCGGTGCAACGTGACCCcgggggcggggg                                                                       >  1:6761759/1‑93 (MQ=255)
                                gATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGGCCCcggggggggggg                                                                       >  1:4544822/1‑93 (MQ=255)
                                 aTGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCACCGGGACCCCGGGCGGGGAggg                                                                      >  2:6348285/1‑101 (MQ=255)
                                  tGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGGCCCCGGGGGGGGGgggg                                                                     >  1:356927/1‑100 (MQ=255)
                                  tGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGgc                                                                     <  2:3093067/101‑1 (MQ=255)
                                   gggCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGgcg                                                                    <  1:5287858/101‑1 (MQ=255)
                                     gCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGcgcg                                                                  <  2:2409432/101‑1 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCACGTGCCCCGGGGGGGGGGGGGGGCggg                                                                >  1:2988039/1‑101 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCACAGTGGCCCGGGGGGGGGGGGGGGGggg                                                                >  1:224671/1‑101 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCGCGGGTCCACCTGGCCCCGGGCGGGGGGGGGGGggg                                                                >  1:1252213/1‑101 (MQ=255)
                                           gcaaTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAg                                                           <  1:4815936/99‑1 (MQ=255)
                                            gAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGa                                                          <  1:6996092/101‑1 (MQ=255)
                                             aaTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGAc                                                         <  2:2697585/101‑1 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGAccc                                                       <  1:6644897/101‑1 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGAccc                                                       <  1:509574/101‑1 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGAccc                                                       <  2:4687599/101‑1 (MQ=255)
                                                 cgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCCACGTGACCCGGGGGGGGGGGGGGGCggggccgaccccc                                                      >  2:2598702/1‑91 (MQ=255)
                                                 cgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCAt                                                     <  2:5474684/101‑1 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGACCCCGGGCGGGGGGGGCGCGGGC.GAGAcacctctc                                                  >  2:1009028/1‑94 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTc                                                  <  2:3745475/101‑1 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTc                                                  <  2:4117976/101‑1 (MQ=255)
                                                      ggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGGCGGGTCAACAGGACCCGGGGGGGGGGGGGGGCGGGCGAGGACCCAtttac                                                 >  1:6933983/1‑99 (MQ=255)
                                                       gctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACCTGACCCGGGGGGGGGGGGGGGCGGGG.GAGACCCCATTCAcc                                               >  1:7110959/1‑101 (MQ=255)
                                                       gctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCAcc                                               <  1:4616162/101‑1 (MQ=255)
                                                         tgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGCCCGGTCCACCG.GAACCGGGGCGGGGGGGGGGGGGGC.AAGGCCCCCTTCAcccg                                             >  2:1808781/1‑99 (MQ=255)
                                                          gggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGTTCAACGTGACCCGGGGGGGGGAGGGGGGGGGG.AAGCGCCGTTTcgcacgg                                            >  2:2306733/1‑95 (MQ=255)
                                                            gTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGt                                          <  2:3591414/101‑1 (MQ=255)
                                                             ttttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGGGACCCCGGGGGGGGGGGGGGCGGGG.AAGACCCATTTCACCCGCggt                                         >  1:6196815/1‑99 (MQ=255)
                                                             ttttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGtt                                         <  1:5949554/101‑1 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTc                                        <  2:5902046/101‑1 (MQ=255)
                                                               ttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTcc                                       <  1:1875701/101‑1 (MQ=255)
                                                               ttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTcc                                       <  1:2438236/101‑1 (MQ=255)
                                                               ttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTcc                                       <  1:4494112/101‑1 (MQ=255)
                                                                tCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCg                                      <  2:3416419/101‑1 (MQ=255)
                                                                 cGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCgg                                     <  1:1614319/101‑1 (MQ=255)
                                                                 cGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCgg                                     <  2:356923/101‑1 (MQ=255)
                                                                   tGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCa                                   <  2:2935552/101‑1 (MQ=255)
                                                                   tGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGGTCAACGTGACCCGGGGCGAGGGGGGGGCGGGG.GAGACCCCTTTTACCGGCGTTCCGGCa                                   >  1:6234143/1‑101 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGCCCGGTTCACCG.GGCCCCGGGCGGGGGGGGGGCGGGG.CAGACCCCATTTACCCGCGGTCCCGCAc                                  >  2:796011/1‑101 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGACCCCGGGCGGGGGGGGCGCCGGC.GAGACCCCATTCACCCGCGCTCCCGccc                                  >  2:114545/1‑99 (MQ=255)
                                                                    gCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCAc                                  <  1:3512156/101‑1 (MQ=255)
                                                                     cTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCAcc                                 <  1:1450411/101‑1 (MQ=255)
                                                                      tGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACcg                                <  1:3086589/101‑1 (MQ=255)
                                                                       gATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGGTCCACGTGACCCCCGGGGAGGGGGGCGCGGGG.GAGACCCACTTCCCCGGCGCTCCGGCACcgg                               >  2:6647129/1‑101 (MQ=255)
                                                                        aTCTATGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGACCCCGGGCGGGGGGGGGGGGGGG.GAGAACCCCTTCACCGGCGGTCCCGCGCccgc                              >  1:6019043/1‑101 (MQ=255)
                                                                         tCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACcggcg                             <  2:6151634/101‑1 (MQ=255)
                                                                          cTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGGGCGGGG.AAGACCCCTTTCACCCGCGTTCCCGCACccgcgg                            >  2:503051/1‑101 (MQ=255)
                                                                           tATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGGTCAACGTGACCCGGGGCGGGGAGGGGGCGGGG.AAGACCCATTTTCCCGGCGGTCCGGCACCGGCggg                           >  1:5402224/1‑101 (MQ=255)
                                                                            aTGCCGCCTCGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGACCCGGGGGGGGGGGGGGGGGGGG.CAGACCCCTTTTACCGGCGGTCCGGCACCGGGgggg                          >  1:2175993/1‑101 (MQ=255)
                                                                               ccgccTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGCCACCGGCGGGGGCg                       <  1:5573035/101‑1 (MQ=255)
                                                                                  ccTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGAtt                    <  1:1003360/101‑1 (MQ=255)
                                                                                   cTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTc                   <  1:5898882/101‑1 (MQ=255)
                                                                                   cTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTc                   <  1:1723198/101‑1 (MQ=255)
                                                                                   cTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTc                   <  1:1797736/101‑1 (MQ=255)
                                                                                    tCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCt                  <  1:4846758/101‑1 (MQ=255)
                                                                                     cGGCCTGTCTGCGGCT.GGGCCGGGTCAACGTGACCCGGGGGGAGGGGGGGGCGGGG.AAGACCCATTTCACCGGCGTTCCGGGCCCGGCggggggggggtcg                 >  2:3225665/1‑93 (MQ=255)
                                                                                       gCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGgc               <  2:2889048/101‑1 (MQ=255)
                                                                                       gCCTGTCTGCGGCG.TGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGgc               <  2:6933610/101‑1 (MQ=255)
                                                                                        ccTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGgcg              <  1:2633673/101‑1 (MQ=255)
                                                                                           gTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCgctg           <  2:2227543/101‑1 (MQ=255)
                                                                                           gTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCgctg           <  1:1208234/101‑1 (MQ=255)
                                                                                             cTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCgctgct         <  1:4035334/101‑1 (MQ=255)
                                                                                              tGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGctgctg        <  1:132855/101‑1 (MQ=255)
                                                                                                cGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  2:1760102/101‑1 (MQ=255)
                                                                                                cGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  2:6748467/101‑1 (MQ=255)
                                                                                                  gCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGGTGCCgg    >  2:6490582/1‑101 (MQ=255)
                                                                                                    t.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCgggc  >  2:1832889/1‑99 (MQ=255)
                                                                                                    t.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTc  <  1:4746775/101‑1 (MQ=255)
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GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCCCGGTTCAACGTGACCCGGGGCGAGGAGGGCGCGGGC.AAGACCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG