Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,193,628 +GGG 100% intergenic (+93/‑158) metK → / → trmB methionine adenosyltransferase/tRNA (guanine‑N(7)‑)‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,193,6281.→G100.0% 44.2 20intergenic (+93/‑158)metK/trmBmethionine adenosyltransferase/tRNA (guanine‑N(7)‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (0/19):  ref base (0/1):  total (0/20)
*NC_0140341,193,6282.→G100.0% 44.2 20intergenic (+93/‑158)metK/trmBmethionine adenosyltransferase/tRNA (guanine‑N(7)‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (0/19):  ref base (0/1):  total (0/20)
*NC_0140341,193,6283.→G100.0% 44.2 20intergenic (+93/‑158)metK/trmBmethionine adenosyltransferase/tRNA (guanine‑N(7)‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (0/19):  ref base (0/1):  total (0/20)

GGTCTGAGCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...GGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGAAAAGCGCAAGACCGGGAAGGGTCGCGGTCAGAG  >  NC_014034/1193529‑1193702
                                                                                                       |||                                                                          
ggTCTGAGCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCcgc...g                                                                           <  1:2751376/101‑1 (MQ=255)
 gTCTGAGCCGGTTTCAAAAGATCACGGGGAAGTGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...gg                                                                          <  1:3404753/101‑1 (MQ=255)
    tGAGCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggg                                                                       <  2:4939140/101‑3 (MQ=255)
     gAGCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCcgccccgcc                                                                         >  1:2346041/1‑95 (MQ=255)
       gCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggct                                                                    <  2:6228329/101‑6 (MQ=255)
       gCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggct                                                                    <  1:1529132/101‑6 (MQ=255)
        ccGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGCTATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctc                                                                   <  2:3453413/101‑7 (MQ=255)
        ccGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctc                                                                   <  2:5027245/101‑7 (MQ=255)
         cGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctcc                                                                  <  1:3565716/101‑8 (MQ=255)
         cGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctcc                                                                  <  1:2846747/101‑8 (MQ=255)
         cGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctcc                                                                  <  2:612959/101‑8 (MQ=255)
         cGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctcc                                                                  <  1:7084261/101‑8 (MQ=255)
         cGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctcc                                                                  <  2:1193052/101‑8 (MQ=255)
          ggTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...ggggggctccg                                                                 >  2:7105085/1‑93 (MQ=255)
                aaaggaccggggggAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGcccccc                                                              <  1:288446/91‑1 (MQ=21)
                aaaaGAGCCGGGGGAAGGGGGCTTCGCCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGcccccc                                                              <  2:3080149/101‑1 (MQ=18)
                 aaaGACCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTGGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCg                                                             <  1:574343/101‑1 (MQ=21)
                 aaaGAACGCGGGGAGGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCg                                                             <  2:83100/101‑1 (MQ=21)
                    gATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                          <  1:5960899/101‑1 (MQ=21)
                      tcgcGGGGAAGGGGGCTCCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                        <  1:3974767/98‑1 (MQ=18)
                      tcccGGGGAGGGGGTCGTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                        <  1:7105459/98‑1 (MQ=18)
                      tCACGGGGAGGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGCCCCGCGGGGGGCTCCGCCCCCCGCAccc                                                        <  2:6599196/101‑1 (MQ=21)
                      tCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                        <  2:249363/101‑1 (MQ=21)
                       cccGGGGAGGGGGGCTGCGGCCCCCTTTTTCATGCGTTGCAGGATATTCCGCCTCGGGCAACCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                       <  2:2276721/99‑1 (MQ=18)
                          ggggAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCg                                                    <  1:4675792/101‑1 (MQ=21)
                                 ggggCTTCGGCCCCTTTTTTCATGCGTTGCAGGATATTTCGCCTGGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTAtt                                             <  2:1551497/101‑1 (MQ=17)
                                   gtcTTCGGCCCCTTTTTTCATGCGTTGCAGGATATTCCCCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTAtttt                                           <  2:2766973/99‑1 (MQ=17)
                                       tCGGCCCCCTTTTTCATGGGTTGCAGGATATTTCGCCTGGGGCAAGCCCGAGG...CGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCa                                       <  1:6601520/101‑1 (MQ=14)
                                       tCGGCCCCCTTTTTAATTGGTTGCCGGAAAATTCCCCCCGGGCAACCCCGGGGCCCCCCCCCGG...GGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCa                                       <  1:7016707/101‑1 (MQ=2)
                                            ccccTTTTTCATGGGTTGCAGGATATTCCGCCGCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGaa                                  <  1:3781416/101‑1 (MQ=14)
                                             cccTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGaaa                                 <  1:3487305/101‑1 (MQ=14)
                                                     actGCGTTGCGGGATATTCCGCCTCGGGCAAGCCCGGGG...CGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGAAAAGCGCAAg                         <  1:3338693/99‑1 (MQ=11)
                                                        ggtttGCAGAAGTTTCCGCCGCGGCCAAGCCCGGGG...CGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGAAAAGCGCAAGAcc                      <  2:1002818/98‑1 (MQ=2)
                                                                            cgggacacccccGAGG...CGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGAAAAGCGCAAGACCGGGAAGGGTCGCGGTCagag  <  2:5106056/92‑1 (MQ=14)
                                                                                                       |||                                                                          
GGTCTGAGCCGGTTTCAAAAGATCACGGGGAAGGGGGCTTCGGCCCCCTTTTTCATGCGTTGCAGGATATTTCGCCTCGGGCAAGCCCGAGG...CGACCCGC...GGGCTCCGCCCCCCGCACCCCCCGGCGTATTTTGCCAAGGAAAAGCGCAAGACCGGGAAGGGTCGCGGTCAGAG  >  NC_014034/1193529‑1193702

Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 39 ≤  ATCG