Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,794 +G 100% intergenic (+3/‑114) trpF → / → trpB1 phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7941.→G100.0% 157.6 289intergenic (+3/‑114)trpF/trpB1phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (85/154):  ref base (36/14):  total (121/168)
Fisher's exact test strand distribution p-value = 2.95e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.28e-07

Only 100 of 326 total aligned reads displayed.

GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889
                                                                                                      |                                                                                                 
gggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGt                                                                                                    <  1:587745/101‑1 (MQ=255)
  gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.gg                                                                                                 <  2:2494319/101‑1 (MQ=255)
  gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.gg                                                                                                 <  1:2580194/101‑1 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.gg.gg                                                                                              <  2:3016407/101‑1 (MQ=255)
      gtCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.gg.gggt                                                                                            <  2:1822299/100‑3 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GG.GGgtt                                                                                           <  2:3588736/101‑1 (MQ=255)
         gcggcgtcGTGTCGGCGGGGGGGGAAGCGGCCTCCGGGGTCAAGGAGGGCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GG.GGgtttc                                                                                         <  2:5245083/94‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GG.GGgtttc                                                                                         <  1:5664699/101‑3 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA.GGGG.GG.GGgtttag                                                                                        >  1:2172637/1‑98 (MQ=255)
            tCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacc                                                                                       <  2:281650/101‑1 (MQ=255)
            gcGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacc                                                                                       <  2:4537588/100‑1 (MQ=255)
             cGACGTGTCGGCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcaccc                                                                                      <  1:1278447/101‑1 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA.GGGTGGG.GGGTTcacccc                                                                                     >  1:4353726/1‑101 (MQ=255)
               aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTcaccccc                                                                                    >  2:1918502/1‑101 (MQ=255)
               aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTcaccccc                                                                                    >  2:3508392/1‑101 (MQ=255)
                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcaccccca                                                                                   <  2:6093457/101‑1 (MQ=255)
                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcaccccca                                                                                   <  2:4238864/101‑1 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GGCGGTTTcacccccac                                                                                  <  1:5573714/101‑1 (MQ=255)
                  tgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTcacccccacc                                                                                 >  1:5475114/1‑100 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTcacccccccac                                                                                >  1:4780140/1‑101 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacccccacac                                                                                <  1:878979/101‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacccccacaccc                                                                              <  1:2261442/101‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.AGGTGGG.GGGTTcaccccccccccc                                                                              >  2:6200448/1‑101 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTtacccccacacccc                                                                             >  1:5944210/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacccccacaccccca                                                                           <  2:5470890/101‑1 (MQ=255)
                        cgGGGGGGGAATCGGCGCCCGGGGTCAACGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTcacccccacaccccca                                                                           <  2:1427701/101‑1 (MQ=255)
                         gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAg                                                                          <  2:127367/101‑1 (MQ=255)
                         gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTcacccccacacccccac                                                                          >  2:4193950/1‑100 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCAGc                                                                         >  2:2189798/1‑101 (MQ=255)
                            ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGt                                                                       <  1:3969408/101‑1 (MQ=255)
                             gTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCCCCCCCCCACCCCCCGCGTa                                                                      >  2:4801569/1‑101 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCCGCGGAttt                                                                   >  2:3892929/1‑101 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTAttt                                                                   <  2:2362714/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCCCCCCCCCAGCCGATTTTg                                                                 >  1:1636614/1‑101 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAAGGGTAGGGGG.GG.GGGTTCACCCCCGCACCCCGAGCgggatttg                                                                 >  2:2030133/1‑94 (MQ=255)
                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGc                                                               <  2:5361048/101‑1 (MQ=255)
                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCGGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCAGCGGATTTTGGcc                                                              >  1:5008706/1‑101 (MQ=255)
                                      gcgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCCGCGGATTTTGGCCa                                                             >  1:1103374/1‑101 (MQ=255)
                                       cgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaa                                                            <  2:4379926/101‑1 (MQ=255)
                                       cgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaa                                                            <  2:5286981/101‑1 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaag                                                           >  2:472381/1‑101 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaag                                                           <  2:2150442/101‑1 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCAGCGTATTTTGGCCaag                                                           >  2:2690206/1‑101 (MQ=255)
                                          ccGGGGTCAAGGGGGCCCCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaa                                                         <  2:648277/101‑1 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCCGCGGATTTTGGCCaagaa                                                         >  1:6217140/1‑101 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCCGCGGATTTTGGCCaagaa                                                         >  1:4132990/1‑101 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                        <  1:755466/101‑1 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                        <  2:3204418/101‑1 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                        <  2:6613635/101‑1 (MQ=255)
                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAagaag.a                                                      <  1:460930/101‑1 (MQ=255)
                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAagaag.a                                                      <  1:1838620/101‑1 (MQ=255)
                                              ggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCCGCGGATTTTGGCCAAGAAG.aaa                                                    >  1:203856/1‑101 (MQ=255)
                                              ggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAG.caa                                                    >  2:3632945/1‑101 (MQ=255)
                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACa                                                  <  2:3761547/101‑1 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAg                                                 <  1:6964810/101‑1 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAgg                                                <  2:851476/101‑1 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAgg                                                <  2:4115390/101‑1 (MQ=255)
                                                   aggaggCCGCCCGTATTGCCTCCTTTGTCGCGGCGGCGCAGGTGTA.GGGT.GGTGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGa                                               <  1:1713895/101‑1 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGa                                               <  1:6897785/101‑1 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGa                                               <  2:5775830/101‑1 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGaa                                              >  2:1600849/1‑101 (MQ=255)
                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAAc                                             <  2:6030385/101‑1 (MQ=255)
                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAAc                                             <  2:3966891/101‑1 (MQ=255)
                                                      aggCCCCCCGGATTGCCGCCTTTGTCGCGGCGGCGGAGGGGTA.GGGTGGG.GGTTTCGCCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACt                                            <  2:354197/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACttt                                          <  1:5333395/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACttt                                          <  1:4401858/101‑1 (MQ=255)
                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTa                                         <  1:6785927/101‑1 (MQ=255)
                                                           gccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTAcc                                       <  2:2618935/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTAccc                                      <  2:5549824/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTAccc                                      <  2:6754825/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTAccc                                      <  2:1542570/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTTAACCCCACCCCCCCCGCGGATTTTTGGCCAGAAGAAAAAAGAAACTTAAcccc                                     >  1:4930109/1‑101 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCg                                    <  2:5946165/101‑1 (MQ=255)
                                                               ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGa                                   >  1:3837161/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGTTCACCCCCACACCCCCCGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAg                                  >  1:3142429/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAg                                  <  2:6123127/101‑1 (MQ=255)
                                                                  ttGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAggg                                >  1:809825/1‑101 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGgc                          <  1:998604/101‑1 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGgc                          >  1:4035441/1‑101 (MQ=255)
                                                                           ttGTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGgcgcg                       >  1:3233445/1‑101 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                     >  1:5758077/1‑101 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                     >  2:113069/1‑101 (MQ=255)
                                                                              tCGCGGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTa                    <  1:6375284/101‑1 (MQ=255)
                                                                               cgcgGCGGCGCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTAt                   >  1:6662969/1‑101 (MQ=255)
                                                                                gcggcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                  <  1:106720/101‑1 (MQ=255)
                                                                                gcggcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                  >  2:6899669/1‑101 (MQ=255)
                                                                                 cggcggcgCAGGGGTA.GGGTGGG.GGGTTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCCATct                 >  1:1853619/1‑101 (MQ=255)
                                                                                  ggcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                >  2:565306/1‑101 (MQ=255)
                                                                                  ggcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                <  1:4215434/101‑1 (MQ=255)
                                                                                  ggcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                >  2:2919542/1‑101 (MQ=255)
                                                                                   gcggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgc               <  1:273037/101‑1 (MQ=255)
                                                                                    cggcgCAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgct              <  2:777494/101‑1 (MQ=255)
                                                                                        gcAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTg          <  1:3678278/101‑1 (MQ=255)
                                                                                         cAGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTgg         <  2:4959080/101‑1 (MQ=255)
                                                                                          aGGGGTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGgc        >  1:5162891/1‑101 (MQ=255)
                                                                                            gggTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGgcgc      <  2:7112753/101‑1 (MQ=255)
                                                                                             ggTA.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGcgcg     >  1:1187394/1‑101 (MQ=255)
                                                                                                a.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  1:5224518/1‑101 (MQ=255)
                                                                                                a.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  <  1:2430073/101‑1 (MQ=255)
                                                                                                a.GGGTGGG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  2:3379059/1‑101 (MQ=255)
                                                                                                      |                                                                                                 
GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA.GGGT.GG.GGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAG.AAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG