Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,253,894 +CCCC 100% intergenic (+52/+39) RCAP_rcc01181 → / ← hisH hypothetical protein/imidazole glycerol phosphate synthase subunit HisH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,253,8941.→C100.0% 80.5 58intergenic (+52/+39)RCAP_rcc01181/hisHhypothetical protein/imidazole glycerol phosphate synthase subunit HisH
Reads supporting (aligned to +/- strand):  new base (49/8):  ref base (0/0):  total (49/9)
*NC_0140341,253,8942.→C100.0% 79.6 59intergenic (+52/+39)RCAP_rcc01181/hisHhypothetical protein/imidazole glycerol phosphate synthase subunit HisH
Reads supporting (aligned to +/- strand):  new base (49/8):  ref base (0/0):  total (49/10)
*NC_0140341,253,8943.→C100.0% 79.9 59intergenic (+52/+39)RCAP_rcc01181/hisHhypothetical protein/imidazole glycerol phosphate synthase subunit HisH
Reads supporting (aligned to +/- strand):  new base (49/8):  ref base (0/0):  total (49/10)
*NC_0140341,253,8944.→C100.0% 79.8 59intergenic (+52/+39)RCAP_rcc01181/hisHhypothetical protein/imidazole glycerol phosphate synthase subunit HisH
Reads supporting (aligned to +/- strand):  new base (49/8):  ref base (0/0):  total (49/10)

CTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGAG.........CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGAAAT  >  NC_014034/1253795‑1253955
                                                                                                               ||||                                                                  
cTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCTCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........c                                                              <  2:4863584/101‑1 (MQ=255)
cTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........c                                                              >  1:4286900/1‑101 (MQ=255)
cTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........c                                                              <  2:6583230/101‑1 (MQ=255)
cTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........c                                                              <  2:3388060/101‑1 (MQ=255)
 tGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cg                                                             >  2:6798983/1‑101 (MQ=255)
 tGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cg                                                             >  2:5118987/1‑101 (MQ=255)
 tGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cg                                                             <  2:6586279/101‑1 (MQ=255)
  gggCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cgg                                                            <  2:4874665/101‑2 (MQ=255)
  gggCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cgg                                                            >  2:5088940/1‑100 (MQ=255)
  gggCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cgg                                                            >  1:159875/1‑100 (MQ=255)
   ggCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cgga                                                           <  1:2607609/101‑3 (MQ=255)
   ggCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cgga                                                           <  1:5119247/101‑3 (MQ=255)
    gCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggag                                                          >  1:5686398/1‑98 (MQ=255)
    gCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggag                                                          <  1:5150533/101‑4 (MQ=255)
    gCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggag                                                          <  1:5892588/101‑4 (MQ=255)
    gCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggag                                                          <  2:4043993/101‑4 (MQ=255)
    gCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggag                                                          >  1:2129304/1‑98 (MQ=255)
     cAAGCTCGTCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagc                                                         <  2:2734914/101‑5 (MQ=255)
      aaGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcc                                                        <  1:6168100/101‑6 (MQ=255)
      aaGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagac                                                        >  2:4426773/1‑96 (MQ=255)
      aaGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....AGGGGGTGCG.....GGGg.........cggagcc                                                        >  1:5620808/1‑96 (MQ=255)
       aGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccc                                                       <  2:2941479/101‑7 (MQ=255)
       aGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccc                                                       <  1:5741023/101‑7 (MQ=255)
        gCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccc                                                      <  2:751006/101‑8 (MQ=255)
        gCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccc                                                      <  2:3628615/101‑8 (MQ=255)
         cTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccccc                                                     <  2:4909667/101‑9 (MQ=255)
         cTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccccc                                                     <  2:2072434/101‑9 (MQ=255)
         cTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccccc                                                     <  1:5251298/101‑9 (MQ=255)
         cTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccccc                                                     <  2:397328/101‑9 (MQ=255)
         cTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagccccc                                                     <  2:852316/101‑9 (MQ=255)
          tCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccccg                                                    >  1:4060888/1‑92 (MQ=38)
          tCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccccg                                                    >  1:3481303/1‑92 (MQ=38)
          tCGGCTTCCAGAACGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccccg                                                    >  1:6308404/1‑92 (MQ=37)
           cGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGg.........cggagcccccgc                                                   >  1:5236487/1‑91 (MQ=38)
                                       gCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGa                                >  1:4686008/1‑101 (MQ=12)
                                       gCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGa                                >  1:6633384/1‑101 (MQ=9)
                                        cGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaa                               >  2:4888439/1‑101 (MQ=12)
                                        cGAAAAACA.CGGATCCCGCCCCTCTCTCATCTTGCCCTAAATACCT.....GGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaa                               <  2:5420628/101‑1 (MQ=2)
                                         gAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              >  2:2652471/1‑101 (MQ=12)
                                         gAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              >  2:2153241/1‑101 (MQ=12)
                                         gAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              >  2:385965/1‑101 (MQ=12)
                                         gAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              >  1:5449076/1‑101 (MQ=12)
                                         gAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGAGCGGCGCCCCCGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              >  2:2380062/1‑101 (MQ=12)
                                          aaaTAAC.CCGCCCCCCCCCCCCTCTCATCTTCCCCTAACTACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAAt                             <  2:599203/101‑1 (MQ=2)
                                          aaaTAAACCCGCCCCCGCCCCGCTCTCATCTTGCCCTAAATCCCG.....GGGGGGTGGGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaaa                              <  2:6528371/101‑1 (MQ=2)
                                          aaaTAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGaact                             >  2:1508987/1‑99 (MQ=9)
                                          aaaTAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAAt                             >  1:5369622/1‑101 (MQ=9)
                                           aaTAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATc                            >  2:358374/1‑101 (MQ=9)
                                            aTAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATcc                           >  1:6269538/1‑101 (MQ=9)
                                             tAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCct                          >  1:2339168/1‑101 (MQ=9)
                                             tAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCct                          >  1:3013033/1‑101 (MQ=9)
                                              taacccaaccccgccccgcTCTCATCTTGCCCTAAATCCCTGGGGGGGGGGGGGGG.....GGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCct                          <  2:3210659/92‑1 (MQ=2)
                                              aaacccacccccgccccgcTCTCATCTTGCCCTAAATCCCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCct                          <  2:1711473/92‑1 (MQ=2)
                                              aaa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctc                         >  1:1626629/1‑101 (MQ=9)
                                              aaa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctc                         >  2:3964083/1‑101 (MQ=9)
                                              aaa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctc                         >  2:3574807/1‑101 (MQ=9)
                                              aaa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctc                         >  2:5772795/1‑101 (MQ=9)
                                               aa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctct                        >  1:727808/1‑101 (MQ=9)
                                               aa.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCctct                        >  1:6587515/1‑101 (MQ=9)
                                                 cccgccccccccccgcTCTCATCTTCCCCTAAATCCCG.....CGGGGGTGCG.....GGGGCGGACCCCCCGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCtctc                       <  2:4751351/95‑1 (MQ=2)
                                                 cccGACCCCGCCCCGCTCTCATCTTGCCCTAAATCCCT.....GGGGGGGGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCtctc                       <  2:3034648/100‑1 (MQ=2)
                                                 cccGACCCCCCCCCGCTCTCATCTTGCCCTAAATCCCT.....CGGGGGTGCG.....GGGGGGGACCCCCCGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCtctc                       <  2:2836045/100‑1 (MQ=9)
                                                  ccGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCtctcc                      >  1:2119061/1‑100 (MQ=9)
                                                   cgcccccgccccgcTCTCATCTTGCCCTAAATCCCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCAc                     <  2:5620518/97‑1 (MQ=2)
                                                    gATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACg                    >  2:6454790/1‑101 (MQ=9)
                                                    gATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACg                    >  2:5101049/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  1:1179820/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  2:316377/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  1:1996565/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  1:3095966/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  1:4584641/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  1:4803610/1‑101 (MQ=9)
                                                     aTCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACgg                   >  2:1139332/1‑101 (MQ=9)
                                                      tCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGc                  >  1:1545029/1‑101 (MQ=9)
                                                          gcccctcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCCATGGGAAATGCTCTCACGGCTTcc              >  2:3222714/1‑101 (MQ=2)
                                                          gccccgcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTcc              >  1:1399708/1‑101 (MQ=9)
                                                          gccccgcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTcc              >  2:5282158/1‑101 (MQ=9)
                                                          cccccgcTCTCATCTTGCCCTAAACCCCT.....CGGGGGTGGGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTcc              <  2:5218709/100‑1 (MQ=9)
                                                           ccccgcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCa             >  2:2669166/1‑101 (MQ=9)
                                                               gcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTc         >  1:6839975/1‑101 (MQ=9)
                                                               gcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTc         >  2:2015444/1‑101 (MQ=9)
                                                               gcTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTc         >  1:3758991/1‑101 (MQ=9)
                                                               gcTCTCATCTTG.CCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCa        >  1:4312414/1‑101 (MQ=2)
                                                                ctctCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCa        >  1:7125039/1‑101 (MQ=9)
                                                                ctctCATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCa        >  2:2603671/1‑101 (MQ=9)
                                                                 tctcATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAg       >  2:5053429/1‑101 (MQ=9)
                                                                 tctcATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAg       >  2:6389894/1‑101 (MQ=9)
                                                                 tctcATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGGTCAg       >  2:2293350/1‑101 (MQ=9)
                                                                  ctcATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAgg      >  2:3575244/1‑101 (MQ=9)
                                                                    cATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCANGGCTTCCAGTTCAggga    >  2:3059400/1‑99 (MQ=9)
                                                                    cATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGaa    >  2:3697327/1‑101 (MQ=9)
                                                                    cATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGaa    >  1:2029490/1‑101 (MQ=9)
                                                                    cATCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGaa    >  1:4424794/1‑101 (MQ=9)
                                                                     aTCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGTCTTCCAGTTCAAGaaa   >  2:459963/1‑101 (MQ=2)
                                                                     aTCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGGGCGGGGCCCCCGCCGGGCCGCCTCGGGGAAAGGCGAGGGGAAATCCTCCCACGGCTTTTAGTTCGGGaaa   >  2:3194037/1‑101 (MQ=2)
                                                                      tCTTGCCCTAAATACCT.....CGGGGGTGCGGGGGCGGAGCCCC.....CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGAAAt  >  2:632473/1‑101 (MQ=9)
                                                                                                               ||||                                                                  
CTGGGCAAGCTCGGCTTCCAGACCGGCTGCGCCTTCGAGGCGAAATAAA.CCGATCCCGCCCCGCTCTCATCTTGCCCTAAATACCT.....CGGGGGTGCG.....GGAG.........CGCCGGGTCGCCTCGGCGAAAGCCGAGGCGAAATCCTCTCACGGCTTCCAGTTCAGGAAAT  >  NC_014034/1253795‑1253955

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG