Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,827 +C 1.1% intergenic (+14/+143) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8272.→C 1.1% 3.7 ‑1.2 ‑0.0369intergenic (+14/+143)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (145/220):  total (148/221)
Fisher's exact test strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.13e-01

Only 100 of 398 total aligned reads displayed.

TATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303727‑1303919
                                                                                                      |                                                                                                
tATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.g                                                                                                   >  2:4772823/1‑101 (MQ=255)
 aTGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..c                                                                                                >  2:6643120/1‑101 (MQ=255)
   gtgCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..ccc                                                                                              <  1:6752582/101‑2 (MQ=255)
     gCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..c                                                                                          <  2:3357406/101‑1 (MQ=255)
      cTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGACGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ct                                                                                         >  1:6274496/1‑100 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctc                                                                                        <  1:3620675/101‑3 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctc                                                                                        <  1:2585524/101‑3 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctc                                                                                        <  1:1822185/101‑3 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctc                                                                                        <  1:1360887/101‑3 (MQ=255)
        cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCGTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcg                                                                                       <  2:4862933/101‑4 (MQ=255)
        cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcg                                                                                       >  1:6031582/1‑98 (MQ=255)
        cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcg                                                                                       <  1:2436837/101‑4 (MQ=255)
         aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGGGCT.G..CCCc..cccgg                                                                                      >  2:1706885/1‑97 (MQ=255)
         aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcgg                                                                                      >  1:6408214/1‑97 (MQ=255)
         aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGACCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcgg                                                                                      >  2:3458550/1‑97 (MQ=255)
            cgcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggctt                                                                                   <  2:3258965/101‑8 (MQ=255)
             gcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggcttc                                                                                  <  2:2177391/101‑9 (MQ=255)
             gcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggcttc                                                                                  <  1:2727057/101‑9 (MQ=255)
             gcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggcttc                                                                                  <  1:283295/101‑9 (MQ=255)
              cggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggcttcg                                                                                 <  1:308552/101‑10 (MQ=255)
              cggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCc..ctcggcttcc                                                                                 >  2:2005009/1‑92 (MQ=255)
                   cggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTcc                                                                              >  2:4144386/1‑100 (MQ=255)
                   cggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCa                                                                              <  2:2783689/101‑1 (MQ=255)
                     gTGGCGATGGAGGGCACGAACGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCAcc                                                                            <  1:5624034/101‑1 (MQ=255)
                        gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCAccccc                                                                         <  2:3105860/101‑1 (MQ=39)
                           aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGGT.G..CCCCCTCGGCTTCGCCTCCCCCCCggg                                                                      >  2:2107327/1‑101 (MQ=255)
                           aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCggg                                                                      >  2:4954505/1‑101 (MQ=39)
                             ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                    <  1:1127353/101‑1 (MQ=38)
                             ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                    <  2:4441274/101‑1 (MQ=38)
                              gAGGGCACGAAGGANAGGCTGTNCCGNGANNCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCA.CCCCGGGatat                                                                  <  1:1952183/101‑1 (MQ=255)
                               aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGatat                                                                  <  1:7126837/101‑1 (MQ=38)
                               aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGatat                                                                  >  2:4314579/1‑101 (MQ=38)
                                 ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCCCCCCCGGGGTGttt                                                                >  2:3733104/1‑101 (MQ=255)
                                 ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATAttt                                                                >  2:5931385/1‑101 (MQ=38)
                                  gCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTg                                                               <  2:552892/101‑1 (MQ=38)
                                   cACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgg                                                              >  2:2603534/1‑101 (MQ=38)
                                   cACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgg                                                              >  2:777247/1‑101 (MQ=38)
                                    aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCCCCCCCGGGATATTTggg                                                             >  2:6074227/1‑101 (MQ=38)
                                    aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATTTTTggg                                                             <  1:5518143/101‑1 (MQ=38)
                                     cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                            >  1:96837/1‑101 (MQ=38)
                                      gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                           >  1:1607249/1‑101 (MQ=38)
                                       aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                          >  1:4721951/1‑101 (MQ=38)
                                        aGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCaa                                                         <  2:1139969/101‑1 (MQ=37)
                                         ggACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAg                                                        <  1:6531755/101‑1 (MQ=37)
                                         ggACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAg                                                        <  2:1332777/101‑1 (MQ=37)
                                          gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                       >  1:2096932/1‑101 (MQ=37)
                                            cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATa                                                     <  2:1548570/101‑1 (MQ=37)
                                             aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaa                                                    <  1:6743424/101‑1 (MQ=37)
                                             aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaa                                                    <  1:4465412/101‑1 (MQ=37)
                                              gCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaaa                                                   <  2:1445404/101‑1 (MQ=37)
                                              gCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCAGGATATTTGGGGCAAGATaaa                                                   <  1:4007742/101‑1 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cc                                                >  2:2922558/1‑101 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cc                                                <  2:3555740/101‑1 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cc                                                <  1:4773063/101‑1 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cc                                                >  2:1856610/1‑101 (MQ=37)
                                                 tGTCCCGCGAAACCCCGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCCCA.GCCCCCC..CGGCTTCGCCCCACCCCCGGGAAACTTCGGGCAAGGTAAA.ccc                                               >  2:5655247/1‑101 (MQ=255)
                                                  gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cccc                                              >  2:912679/1‑101 (MQ=37)
                                                  gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.cccc                                              <  1:5207602/101‑1 (MQ=37)
                                                    cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCAt                                            >  2:6448059/1‑101 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATc                                           >  2:5263960/1‑101 (MQ=37)
                                                      cgcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATcc                                          <  2:5341691/101‑1 (MQ=37)
                                                       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCt                                         <  2:6838630/101‑1 (MQ=37)
                                                       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCt                                         <  2:5228194/101‑1 (MQ=37)
                                                         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCttt                                       <  2:2420163/101‑1 (MQ=37)
                                                         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCttt                                       <  2:6274173/101‑1 (MQ=37)
                                                          aatcccGGCGCGCCGTGGTCAGCGTTTAGGGAGTGGGCGGAT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAGAAA.CCCCATCCTTTc                                      <  2:2657500/98‑1 (MQ=19)
                                                           aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCa                                     <  2:1222676/101‑1 (MQ=37)
                                                            aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCAc                                    <  1:6643427/101‑1 (MQ=37)
                                                             cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGGTATTTGGGGCAAGATAAA.CCCCATCCTTTCAcc                                   >  1:5321061/1‑101 (MQ=37)
                                                             cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCAcc                                   <  2:4210834/101‑1 (MQ=37)
                                                               cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGG..CCCCT.CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCtt                                 >  1:5841763/1‑101 (MQ=37)
                                                                tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTg                                <  2:4587953/101‑1 (MQ=37)
                                                                 gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGc                               <  2:1647919/101‑1 (MQ=37)
                                                                   gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGccc                             >  1:2241738/1‑100 (MQ=25)
                                                                      ccGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCaa                          <  1:5138087/101‑1 (MQ=37)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        >  1:3186975/1‑101 (MQ=25)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        >  1:3149922/1‑101 (MQ=37)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        >  1:3361717/1‑101 (MQ=25)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        <  1:4264338/101‑1 (MQ=37)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        <  1:6895626/101‑1 (MQ=37)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTAACCTTGCCGCAAat                        <  1:1693955/101‑1 (MQ=25)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCACGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAat                        <  1:6979862/101‑1 (MQ=37)
                                                                          ggTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAatat                      >  2:511467/1‑101 (MQ=25)
                                                                          ggTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAatat                      <  2:5820105/101‑1 (MQ=37)
                                                                          ggTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAatat                      >  2:6144770/1‑101 (MQ=25)
                                                                          ggTCAGCGTTTAGGCAAGGGCGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTTGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCTCAAatat                      <  1:4381826/101‑1 (MQ=25)
                                                                          ggTCAGCGTTGAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAatat                      <  2:5683145/101‑1 (MQ=25)
                                                                           gTCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATc                     <  2:3186859/101‑1 (MQ=37)
                                                                            tCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATcc                    >  2:752196/1‑101 (MQ=37)
                                                                            tCAGCGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATcc                    >  2:2500890/1‑101 (MQ=25)
                                                                                cGTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCggg                >  1:4665957/1‑101 (MQ=25)
                                                                                 gTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCgggg               <  1:2891975/101‑1 (MQ=37)
                                                                                 gTTTAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCgggg               >  1:56894/1‑101 (MQ=25)
                                                                                  tttAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCggggg              >  1:1012531/1‑101 (MQ=25)
                                                                                   ttAGGCAAGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCgggggg             <  1:593787/101‑1 (MQ=37)
                                                                                    tAGGCAAGGGGGCGAT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCCAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCggggggg            >  1:202799/1‑100 (MQ=25)
                                                                                         aaGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       >  2:3931161/1‑101 (MQ=25)
                                                                                         aaGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       >  1:3731523/1‑101 (MQ=25)
                                                                                          aGGGGGCGCT.G..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg      >  1:6171752/1‑101 (MQ=25)
                                                                                           gggggCTCT.GC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca  <  2:6974132/101‑2 (MQ=2)
                                                                                                      |                                                                                                
TATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCT.G..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303727‑1303919

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG