Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,683 +C 6.0% intergenic (+495/‑436) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6831.→C 6.0% 59.8 ‑0.2 ‑0.0366intergenic (+495/‑436)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/9):  ref base (187/157):  total (200/166)
Fisher's exact test strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 422 total aligned reads displayed.

GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395585‑1395783
                                                                                                    |                                                                                                      
gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..Gc                                                                                                     <  1:6128623/101‑1 (MQ=255)
gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..Gc                                                                                                     <  2:4445359/101‑1 (MQ=255)
  gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAg                                                                                                   >  1:116810/1‑101 (MQ=255)
  gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAg                                                                                                   >  1:5892832/1‑101 (MQ=255)
  gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAg                                                                                                   >  1:2766952/1‑101 (MQ=255)
    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGtt                                                                                                 >  2:2118791/1‑101 (MQ=255)
      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCg                                                                                               <  2:2617516/101‑1 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTTCATGTAGTCGGCG.G..GCAGTTCGa                                                                                              >  2:832486/1‑101 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGa                                                                                              >  2:1536366/1‑101 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.Gc                                                                                           >  2:1132333/1‑101 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGAGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.Gc                                                                                           >  1:5069399/1‑101 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                         >  1:1866102/1‑101 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                         <  2:4743179/101‑1 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                         >  2:4010374/1‑101 (MQ=255)
            tcagtcatGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGc                                                                                        >  2:3267065/8‑101 (MQ=255)
             ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCa                                                                                       <  1:134279/101‑1 (MQ=255)
              caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAg                                                                                      >  2:3810991/1‑101 (MQ=255)
                ccTTGCGGATTGTCACTGGCGCGGGGCAAATCGTCGCACCCCACGCGGGGACGAGAATCGAAACATCTGAATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTc                                                                                    <  2:1269876/101‑1 (MQ=255)
                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCg                                                                                   <  1:6530957/101‑1 (MQ=255)
                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCg                                                                                   >  2:4660863/1‑101 (MQ=255)
                   tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCt                                                                                 <  2:1808573/101‑1 (MQ=255)
                    gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                >  2:5014717/1‑101 (MQ=255)
                    gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                <  2:6331316/101‑1 (MQ=255)
                      ggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTccc                                                                              <  1:43831/101‑1 (MQ=255)
                       gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccc                                                                             <  1:5267469/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                           <  2:3452774/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                           >  2:6807326/1‑101 (MQ=255)
                          tGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                          <  2:5631423/101‑1 (MQ=255)
                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                         <  2:6088594/101‑1 (MQ=255)
                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                         >  2:6723088/1‑101 (MQ=255)
                               cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                                     >  2:1408783/1‑101 (MQ=255)
                                 ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                   <  1:2177481/101‑1 (MQ=255)
                                   cgcgGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                                 <  1:6265978/101‑1 (MQ=255)
                                     ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                >  2:1700774/10‑101 (MQ=2)
                                     cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:4270277/101‑1 (MQ=255)
                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:2594620/101‑1 (MQ=255)
                                      gggccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:1446459/93‑1 (MQ=2)
                                       gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:2771258/101‑1 (MQ=255)
                                       gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:4010549/101‑1 (MQ=255)
                                        tcactcgagccGCTCCCCCCGGGGGGGCGTGGTGCAAAACATCGGCTTGTCCGCTTCGAA..CCTGGTCGC.GTCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:2322139/91‑1 (MQ=2)
                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:3071928/101‑1 (MQ=255)
                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:2457405/101‑1 (MQ=255)
                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGGAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:2216234/101‑1 (MQ=255)
                                        gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:4224277/95‑1 (MQ=2)
                                        gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:4318858/7‑101 (MQ=2)
                                          cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:1221912/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  2:1940605/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:105771/1‑101 (MQ=255)
                                            aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:5418038/1‑101 (MQ=255)
                                             tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:3942573/2‑101 (MQ=2)
                                             tgCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCGCCGCGGGGGCGTGGATCGagc                                                        >  2:5848258/2‑99 (MQ=2)
                                             aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  1:5394979/1‑101 (MQ=255)
                                              gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:5658248/101‑1 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTG.GCAGGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGAGCGAAAcc                                                      >  2:6877772/1‑100 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCATTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                   <  1:4585699/101‑1 (MQ=255)
                                                   ccTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                 <  2:1867012/100‑1 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCTTGTCCGCATCGAA..GCTGGTCAC.ATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggcgta                                                 <  1:4558574/101‑7 (MQ=2)
                                                     tCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGCCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                               <  1:832510/101‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                              >  1:98547/1‑101 (MQ=255)
                                                       cccccGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                             <  2:523025/101‑1 (MQ=255)
                                                          ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                          <  1:6005930/101‑1 (MQ=255)
                                                          ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                          >  2:1213817/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                         <  2:4579664/101‑1 (MQ=255)
                                                             ggggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                       <  1:4895420/100‑1 (MQ=255)
                                                              gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                      >  2:4899301/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                     >  1:1659862/1‑101 (MQ=255)
                                                                  gCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                  <  2:2900898/101‑1 (MQ=255)
                                                                  gCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                  <  1:3238987/101‑1 (MQ=255)
                                                                   tgtagATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGCATCTGGCGCAAACg                                 >  1:6837709/5‑101 (MQ=255)
                                                                      ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                              <  2:2487973/101‑1 (MQ=255)
                                                                      ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                              >  2:4091292/1‑101 (MQ=255)
                                                                      ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                              <  2:6506740/101‑1 (MQ=255)
                                                                        acaaaacgctCTGCCTGTCGTGGCGG.G..GAAGTGGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                            <  2:1640120/92‑1 (MQ=21)
                                                                         tCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt                           <  2:2811739/101‑1 (MQ=255)
                                                                         tCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt                           >  1:668273/1‑101 (MQ=255)
                                                                         tCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt                           >  2:6261544/1‑101 (MQ=255)
                                                                          cGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                          >  2:6994369/1‑101 (MQ=255)
                                                                            aaaCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                        <  1:356010/101‑1 (MQ=255)
                                                                            aaaCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                        >  1:2963383/1‑101 (MQ=255)
                                                                             accttCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                       <  1:4079946/97‑1 (MQ=255)
                                                                             aaCATCTGCATGTAGGCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                       <  1:1107614/101‑1 (MQ=255)
                                                                              aCATCTGCATGTAGTCGGCG.G..GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                      <  1:5130700/101‑1 (MQ=255)
                                                                               cATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                     >  1:6370234/1‑101 (MQ=255)
                                                                                aTCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTNTGGATCTGGCGCAAACGGAAAGTCTgtcgt                    >  1:1984913/1‑101 (MQ=255)
                                                                                aTCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                    >  1:6627045/1‑101 (MQ=255)
                                                                                 tCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                   <  1:2349569/101‑1 (MQ=255)
                                                                                 tCTGCATGGAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                   <  1:1838428/101‑1 (MQ=255)
                                                                                  cTGCAGGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                  <  2:3415184/101‑1 (MQ=255)
                                                                                   tGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                 >  2:5899082/1‑101 (MQ=255)
                                                                                      aTGTGGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc              <  1:670441/101‑1 (MQ=255)
                                                                                      aTGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc              <  1:7010128/101‑1 (MQ=255)
                                                                                       tGTAGTCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAACGTCTGTCGTCGCTccc             >  2:5085261/1‑101 (MQ=255)
                                                                                          agccgggg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc          <  2:6594548/98‑1 (MQ=255)
                                                                                          aGTCGGCT.G..GAAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc          <  1:4659912/101‑1 (MQ=255)
                                                                                           gTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg         >  1:387934/1‑101 (MQ=255)
                                                                                                cg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg    <  1:3424807/101‑1 (MQ=255)
                                                                                                cg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg    >  2:3157127/1‑101 (MQ=255)
                                                                                                 g.g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg   >  2:3620416/1‑101 (MQ=255)
                                                                                                   g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc  <  1:6783428/101‑1 (MQ=255)
                                                                                                   g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc  <  1:1536426/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395585‑1395783

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG